C-S lyase activities in leaves of crucifers and non-crucifers, and the characterization of three classes of C-S lyase activities from oilseed rape (Brassica napus L.)

A - Papers appearing in refereed journals

Kiddle, G. A., Bennett, R. N., Hick, A. J. and Wallsgrove, R. M. 1999. C-S lyase activities in leaves of crucifers and non-crucifers, and the characterization of three classes of C-S lyase activities from oilseed rape (Brassica napus L.). Plant, Cell and Environment. 22 (5), pp. 433-445. https://doi.org/10.1046/j.1365-3040.1999.00416.x

AuthorsKiddle, G. A., Bennett, R. N., Hick, A. J. and Wallsgrove, R. M.
Abstract

C-S lyases in plants are involved in primary and secondary metabolism, and in glucosinolate-containing species may be involved in glucosinolate biosynthesis. Extracts from oilseed rape (Brassica napus) leaves were assayed for several C-S lyase activities. Pour activities [using L-cystine, L-cystathionine, S-(2-benzothiazolyl)-L-Cys (SBC) and S-benzyl-L-Cys] were investigated in detail. All are developmentally regulated (highest in youngest leaves), and differentially inhibited by iodoacetamide, N-ethylmaleimide (NEM) and ethylenediaminetetraacetic acid (EDTA). Thermal stabilities and pH optima were also distinct, Competitive inhibition of the SEC lyase activity with a variety of sulphur-containing compounds indicated that cystine lyase contributes to SEC degradation, and this enzyme may cleave a wide range of compounds, both aliphatic and aromatic, but other 'SBC lyases' were also present, Putative aromatic glucosinolate intermediates were cleaved by the rape enzymes. Developmental and biochemical studies indicate that at least three classes of C-S lyase activity are present in rape leaves: cystathionine beta-lyase, cystine lyase and a group of relatively non-specific lyases, C-S lyase. preparations from other glucosinolate- and non-glucosinolate-containing species were capable of cleaving a number of aliphatic and aromatic conjugates, The highest activities were detected in glucosinolate-containing species and Allium cepa (onion). C-S lyase activities in non-glucosinolate-containing species (tobacco, Nicotiana tabacum, and barley, Hordeum vulgare) were much lower.

KeywordsPlant Sciences
Year of Publication1999
JournalPlant, Cell and Environment
Journal citation22 (5), pp. 433-445
Digital Object Identifier (DOI)https://doi.org/10.1046/j.1365-3040.1999.00416.x
Open accessPublished as non-open access
Funder project or code215
413
437
Project: 011281
ISSN01407791
PublisherWiley

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