Reporter genes

Huttly, A. K. 2008. Reporter genes. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 39-69

Related outputs

A high soluble-fibre allele in wheat encodes a defective cell wall peroxidase responsible for dimerization of ferulate moieties on arabinoxylan

A - Papers appearing in refereed journals

Mitchell, R. A. C., Oszvald, M., Pellny, T. K., Freeman, J., Halsey, K., Sparks, C. A., Huttly, A. K., Specel, S., Leverington-Waite, M., Griffiths, S., Shewry, P. R. and Lovegrove, A. 2023. A high soluble-fibre allele in wheat encodes a defective cell wall peroxidase responsible for dimerization of ferulate moieties on arabinoxylan. bioRxiv. https://doi.org/10.1101/2023.03.08.531735

Proanthocyanidin Biosynthesis in the Developing Wheat Seed Coat Investigated by Chemical and RNA-seq Analysis

A - Papers appearing in refereed journals

Phillips, A. L., Vaughan, S., Baker, J., Primavesi, L. F., Patil, A., King, R., Hassani-Pak, K., Kulasekaran, S., Coghill, J., Ward, J. L. and Huttly, A. K. 2022. Proanthocyanidin Biosynthesis in the Developing Wheat Seed Coat Investigated by Chemical and RNA-seq Analysis. Plant Direct. 6 (10), p. e453. https://doi.org/10.1002/pld3.453

Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2

A - Papers appearing in refereed journals

Raffan, S., Sparks, C. A., Huttly, A. K., Hyde, L., Martignago, D., Mead, A., Hanley, S. J., Wilkinson, P., Barker, G., Edwards, K. J., Curtis, T. Y., Usher, S. L., Kosik, O. and Halford, N. G. 2021. Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2 . Plant Biotechnology Journal. 19 (8), pp. 1602-1613. https://doi.org/10.1111/pbi.13573

Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain

A - Papers appearing in refereed journals

Wan, Y., Wang, Y., Shi, Z., Rentsch, D., Ward, J. L., Hassall, K. L., Sparks, C. A., Huttly, A. K., Buchner, P. H., Powers, S. J., Shewry, P. R. and Hawkesford, M. J. 2021. Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain. PLOS ONE. 16 (2), p. e0246763. https://doi.org/10.1371/journal.pone.0246763

Use of a modified CRISPR/Cas9 construct to simultaneously mutagenize multiple genes in hexaploid wheat

C1 - Edited contributions to conferences/learned societies

Winfield, M., Allen, S., Cartlidge, L., Sparks, C. A., Jones, H., Huttly, A. K., Martignago, D., Hawkesford, M. J. and Edwards, K. 2017. Use of a modified CRISPR/Cas9 construct to simultaneously mutagenize multiple genes in hexaploid wheat. MonoGram 2017, 4-6 April, University of Bristol. Bristol, University of Bristol.

Opportunities for research via wheat transformation

C2 - Non-edited contributions to conferences

Sparks, C. A., Martignago, D., Huttly, A. K., Raffan, S., Doherty, A. and Edwards, K. 2017. Opportunities for research via wheat transformation. The 5th Plant Genomics and Gene Editing Congress, Amsterdam, 16–17 March 2017. Societas Physiologiae Plantarum Scandinavica.

Heterologous expression and transcript analysis of gibberellin biosynthetic genes of grasses reveals novel functionality in the GA3ox family

A - Papers appearing in refereed journals

Pearce, S., Huttly, A. K., Prosser, I. M., Li, Y-D., Vaughan, S. P., Gallova, B., Patil, A., Coghill, J. A., Dubcovsky, J., Hedden, P. and Phillips, A. L. 2015. Heterologous expression and transcript analysis of gibberellin biosynthetic genes of grasses reveals novel functionality in the GA3ox family. BMC Plant Biology. 15, p. 130. https://doi.org/10.1186/s12870-015-0520-7

Modelling wheat breakage during roller milling using the Double Normalised Kumaraswamy Breakage Function: effects of kernel shape and hardness

A - Papers appearing in refereed journals

Campbell, G. M., Sharp, C., Wall, K., Mateos-Salvador, F., Gubatz, S., Huttly, A. K. and Shewry, P. R. 2012. Modelling wheat breakage during roller milling using the Double Normalised Kumaraswamy Breakage Function: effects of kernel shape and hardness. Journal of Cereal Science. 55, pp. 415-425. https://doi.org/10.1016/j.jcs.2012.02.002

The role of gibberellin in determining wheat grain size and quality

C2 - Non-edited contributions to conferences

Vaughan, S. P., Wanchoo-Kohli, A., Patil, A., Huttly, A. K., Phillips, A. L. and Hedden, P. 2011. The role of gibberellin in determining wheat grain size and quality. ABRATES.

The genetics and biochemistry of wheat grain colour

C2 - Non-edited contributions to conferences

Vaughan, S. P., Baker, J., Ward, J. L., Huttly, A. K. and Phillips, A. L. 2011. The genetics and biochemistry of wheat grain colour. ABRATES.

The role of gibberellin in determining wheat grain size and quality

C1 - Edited contributions to conferences/learned societies

Vaughan, S. P., Wanchoo-Kohli, A., Patil, A., Huttly, A. K., Phillips, A. L. and Hedden, P. 2011. The role of gibberellin in determining wheat grain size and quality. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 14

Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm

C1 - Edited contributions to conferences/learned societies

Freeman, J., Kurup, S., Huttly, A. K. and Shewry, P. R. 2008. Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 453-455

Transcriptome analysis of grain development in hexaploid wheat

A - Papers appearing in refereed journals

Wan, Y., Poole, R. L., Huttly, A. K., Toscano-Underwood, C., Feeney, K., Welham, S. J., Gooding, M. J., Mills, C., Edwards, K. J., Shewry, P. R. and Mitchell, R. A. C. 2008. Transcriptome analysis of grain development in hexaploid wheat. BMC Genomics. 9, p. 121 (16pp). https://doi.org/10.1186/1471-2164-9-121

The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations

A - Papers appearing in refereed journals

Pastori, G. M., Huttly, A. K., West, J., Sparks, C. A., Pieters, A., Luna, C. M., Jones, H. D. and Foyer, C. H. 2007. The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations. Functional Plant Biology. 34 (9), pp. 835-843. https://doi.org/10.1071/FP07112

Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm

C2 - Non-edited contributions to conferences

Freeman, J., Kurup, S., Huttly, A. K. and Shewry, P. R. 2007. Using cell-lineage tagging to decipher early differentiation and development in wheat endosperm. Abstracts 4th UK Small Grain Cereals Genetic and Genomics Workshop: From Genes to Traits, Cambridge, 14-16 March 2007 .

Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins

C1 - Edited contributions to conferences/learned societies

Shewry, P. R., Tosi, P., Jones, H. D., Sparks, C. A., Gritsch, C., Funatsuki, W., Niwa, K., Huttly, A. K., Napier, J. A., D'ovidio, R. and Freeman, J. 2007. Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins. Lookhart, G. L. and Ng, P. W. K. (ed.) Gluten proteins 2006 . American Association of Cereal Chemists (AACC), St Paul, MN. pp. 112-116

Aphid alarm pheromone produced by transgenic plants affects aphid and parasitoid behavior

A - Papers appearing in refereed journals

Beale, M. H., Birkett, M. A., Bruce, T. J. A., Chamberlain, K., Field, L. M., Huttly, A. K., Martin, J. L., Parker, R., Phillips, A. L., Pickett, J. A., Prosser, I. M., Shewry, P. R., Smart, L. E., Wadhams, L. J., Woodcock, C. M. and Zhang, Y. 2006. Aphid alarm pheromone produced by transgenic plants affects aphid and parasitoid behavior. Proceedings of the National Academy of Sciences. 103, pp. 10509-10513. https://doi.org/10.1073/pnas.0603998103

CLAVATA1 dominant-negative alleles reveal functional overlap between multiple receptor kinases that regulate meristem and organ development

A - Papers appearing in refereed journals

Dievert, A., Dalal, M., Tax, F. E., Lacey, A. D., Huttly, A. K., Li, J. and Clark, S. E. 2003. CLAVATA1 dominant-negative alleles reveal functional overlap between multiple receptor kinases that regulate meristem and organ development. The Plant Cell. 15 (5), pp. 1198-1211. https://doi.org/10.​1105/​tpc.​010504

Novel anther-specific myb genes from tobacco as putative regulators of phenylalanine ammonia-lyase expression

A - Papers appearing in refereed journals

Yang, S., Sweetman, J. P., Amirsadeghi, S., Barghchi, M., Huttly, A. K., Chung, W-I. and Twell, D. 2001. Novel anther-specific myb genes from tobacco as putative regulators of phenylalanine ammonia-lyase expression. Plant Physiology. 126, pp. 1738-1753.

Hormonal and developmental regulation of alpha-amylase in transgenic wheat

C2 - Non-edited contributions to conferences

Stone, M. C., Wilkinson, M. D., Huttly, A. K., Lenton, J. R. and Jones, H. D. 2001. Hormonal and developmental regulation of alpha-amylase in transgenic wheat. Abstracts SEB Annual Meeting, Canterbury, 2-6 April 2001 . pp. P7.78

Isolation and Expression of Three Gibberellin 20-Oxidase cDNA Clones from Arabidopsis

A - Papers appearing in refereed journals

Phillips, A. L., Ward, D. A., Uknes, S., Appleford, N. E. J., Lange, T., Huttly, A. K., Gaskin, P., Graebe, J. E. and Hedden, P. 1995. Isolation and Expression of Three Gibberellin 20-Oxidase cDNA Clones from Arabidopsis. Plant Physiology. 108 (3), pp. 1049-1057. https://doi.org/10.1104/pp.108.3.1049