The Arabidopsis RRM domain protein EDM3 mediates race-specific disease resistance by controlling H3K9me2-dependent alternative polyadenylation of RPP7 immune receptor transcripts.

A - Papers appearing in refereed journals

Lai, Y., Cuzick, A., Lu, X., Wang, J., Katiyar, N., Tsuchiya, T., Le Roch, K., McDowell, J., Holub, E. and Eulgem, T. 2019. The Arabidopsis RRM domain protein EDM3 mediates race-specific disease resistance by controlling H3K9me2-dependent alternative polyadenylation of RPP7 immune receptor transcripts. The Plant Journal. 97 (4), pp. 646-660. https://doi.org/10.1111/tpj.14148

AuthorsLai, Y., Cuzick, A., Lu, X., Wang, J., Katiyar, N., Tsuchiya, T., Le Roch, K., McDowell, J., Holub, E. and Eulgem, T.
Abstract

The NLR-receptor RPP7 mediates race-specific immunity in Arabidopsis. Previous screens for enhanced downy mildew (edm) mutants identified the co-chaperone SGT1b (EDM1) and the PHD-finger protein EDM2 as critical regulators of RPP7. Here, we describe a third edm mutant compromised in RPP7 immunity, edm3. EDM3 encodes a nuclear-localized protein featuring an RNA-recognition motif. Like EDM2, EDM3 promotes histone H3 lysine 9 dimethylation (H3K9me2) at RPP7. Global profiling of H3K9me2 showed EDM3 to affect this silencing mark at a large set of loci. Importantly, both EDM3 and EDM2 co-associate in vivo with H3K9me2-marked chromatin and transcripts at a critical proximal polyadenylation site of RPP7, where they suppress proximal transcript polyadeylation/termination. Our results highlight the complexity of plant NLR gene regulation, and establish a functional and physical link between a histone mark and NLR-transcript processing.

KeywordsHyaloperonospora arabidopsidis ; RNA-binding protein; Disease-resistance genes; Histone-binding proteins; Transcript processing
Year of Publication2019
JournalThe Plant Journal
Journal citation97 (4), pp. 646-660
Digital Object Identifier (DOI)https://doi.org/10.1111/tpj.14148
PubMed ID30407670
Web address (URL)https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.14148
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
US National Science Foundation
Funder project or code207/ICR07554
IOS-1457329
Accepted author manuscript
Output statusPublished
Publication dates
Online08 Nov 2018
Publication process dates
Accepted28 Oct 2018
Copyright licenseCC BY
PublisherWiley-Blackwell
ISSN0960-7412

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