A - Papers appearing in refereed journals
Jenkinson, D. S. and Oades, J. M. 1979. A method for measuring adenosine triphosphate in soil. Soil Biology and Biochemistry. 11 (2), pp. 193-1999. https://doi.org/10.1016/0038-0717(79)90100-7
Authors | Jenkinson, D. S. and Oades, J. M. |
---|---|
Abstract | A method was devised for the extraction and measurement of adenosine 5'-triphosphate (ATP) in soil that minimizes sorption of ATP on the soil colloids. Soil was ultrasonified for 1 min with a solution containing trichloracetic acid (0.5 m). disodium hydrogen orthophosphate (0.25 m) and paraquat dichloride (0.1 m). The ATP content of the filtered extract was determined without further treatment in a scintillation spectrometer by the firefly luciferin-luciferase system. Recovery of added ATP was greater using the extratant containing trichloracetic acid, orthophosphate and paraquat than with trichloracetic acid alone or with a sulphuric acid extradant. Recoveries of added ATP ranged from 45% to 84% in thirteen different soils; ATP contents from 0.64 to 9.03 μg g−1 soil. |
Year of Publication | 1979 |
Journal | Soil Biology and Biochemistry |
Journal citation | 11 (2), pp. 193-1999 |
Digital Object Identifier (DOI) | https://doi.org/10.1016/0038-0717(79)90100-7 |
Open access | Published as non-open access |
Output status | Published |
Publication dates | |
1979 | |
Online | 12 Nov 2002 |
Publication process dates | |
Accepted | 17 Jul 1978 |
Copyright license | Publisher copyright |
ISSN | 0038-0717 |
Publisher | Elsevier |
Permalink - https://repository.rothamsted.ac.uk/item/8ww5z/a-method-for-measuring-adenosine-triphosphate-in-soil