A method for measuring adenosine triphosphate in soil

A - Papers appearing in refereed journals

Jenkinson, D. S. and Oades, J. M. 1979. A method for measuring adenosine triphosphate in soil. Soil Biology and Biochemistry. 11 (2), pp. 193-1999.

AuthorsJenkinson, D. S. and Oades, J. M.
Abstract

A method was devised for the extraction and measurement of adenosine 5'-triphosphate (ATP) in soil that minimizes sorption of ATP on the soil colloids. Soil was ultrasonified for 1 min with a solution containing trichloracetic acid (0.5 m). disodium hydrogen orthophosphate (0.25 m) and paraquat dichloride (0.1 m). The ATP content of the filtered extract was determined without further treatment in a scintillation spectrometer by the firefly luciferin-luciferase system. Recovery of added ATP was greater using the extratant containing trichloracetic acid, orthophosphate and paraquat than with trichloracetic acid alone or with a sulphuric acid extradant. Recoveries of added ATP ranged from 45% to 84% in thirteen different soils; ATP contents from 0.64 to 9.03 μg g−1 soil.

Year of Publication1979
JournalSoil Biology and Biochemistry
Journal citation11 (2), pp. 193-1999
Digital Object Identifier (DOI)doi:10.1016/0038-0717(79)90100-7
Open accessPublished as non-open access
Output statusPublished
Publication dates
Print1979
Online12 Nov 2002
Publication process dates
Accepted17 Jul 1978
Copyright licensePublisher copyright
ISSN0038-0717
PublisherElsevier

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