Adenosine triphosphate measurement in soil: an improved method

A - Papers appearing in refereed journals

Tate, K. R. and Jenkinson, D. S. 1982. Adenosine triphosphate measurement in soil: an improved method. Soil Biology and Biochemistry. 14 (4), pp. 331-335.

AuthorsTate, K. R. and Jenkinson, D. S.
Abstract

An improved method for measuring the adenosine 5'-triphosphate (ATP) content of soil is described, in which a purified rather than a crude luciferase preparation is used. Light produced during the reaction between luciferase and ATP is measured with a liquid scintillation spectrometer. Calibration curves by the modified method are linear. Guanosine 5'-triphosphate (GTP) increased light output with the crude but not with the purified luciferase, presumably because the crude preparation contained enzymes that converted GTP to ATP. The ATP contents of 9 soils were, on average, 24% lower when measured using the purified preparation of luciferase than when measured using the crude preparation. However the amount of GTP needed to account for the difference was unreasonably large and other ATP-synthesizing processes must have contributed to the extra ATP measured with the crude luciferase. Conditions for using a luminometer for measuring light output in the ATP assay procedure, in place of a liquid scintillation spectrometer, are also described.

Year of Publication1982
JournalSoil Biology and Biochemistry
Journal citation14 (4), pp. 331-335
Digital Object Identifier (DOI)doi:10.1016/0038-0717(82)90002-5
Open accessPublished as non-open access
Output statusPublished
Publication dates
Online12 Nov 2002
Publication process dates
Accepted01 Dec 1981
Copyright licensePublisher copyright
ISSN0038-0717
PublisherElsevier

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