Potassium channels in barley: cloning, functional characterization and expression analyses in relation to leaf growth and development

A - Papers appearing in refereed journals

Boscari, A., Clement, M., Volkov, V., Golldack, D., Hybiak, J., Miller, A. J., Amtmann, A. and Fricke, W. 2009. Potassium channels in barley: cloning, functional characterization and expression analyses in relation to leaf growth and development. Plant, Cell and Environment. 32 (12), pp. 1761-1777.

AuthorsBoscari, A., Clement, M., Volkov, V., Golldack, D., Hybiak, J., Miller, A. J., Amtmann, A. and Fricke, W.
Abstract

It is not known how the uptake and retention of the key osmolyte K+ in cells are mediated in growing leaf tissue. In the present study on the growing leaf 3 of barley, we have cloned the full-length coding sequence of three genes which encode putative K+ channels (HvAKT1, HvAKT2, HvKCO1/HvTPK1), and of one gene which encodes a putative K+ transporter (HvHAK4). The functionality of the gene products of HvAKT1 and HvAKT2 was tested through expression in Xenopus laevis oocytes. Both are inward-rectifying K+ channels which are inhibited by Cs+. Function of HvAKT1 in oocytes requires co-expression of a calcineurin-interacting protein kinase (AtCIPK23) and a calcineurin B-like protein (AtCBL9) from Arabidopsis, showing cross-species complementation of function. In planta, HvAKT1 is expressed primarily in roots, but is also expressed in leaf tissue. HvAKT2 is expressed particularly in leaf tissue, and HvHAK4 is expressed particularly in growing leaf tissue. Within leaves, HvAKT1 and HvAKT2 are expressed predominantly in mesophyll. Expression of genes changes little in response to low external K+ or salinity, despite major changes in K+ concentrations and osmolality of cells. Possible contributions of HvAKT1, HvAKT2, HvKCO1 and HvHAK4 to regulation of K+ relations of growing barley leaf cells are discussed.

KeywordsPlant Sciences
Year of Publication2009
JournalPlant, Cell and Environment
Journal citation32 (12), pp. 1761-1777
Digital Object Identifier (DOI)doi:10.1111/j.1365-3040.2009.02033.x
PubMed ID19682291
Open accessPublished as non-open access
FunderIRCSET, Ireland
Leverhulme Trust
Biotechnology and Biological Sciences Research Council
Funder project or codeSEF
Centre for Crop Genetic Improvement (CGI)
Project: 4967
Optimising nutrient use in cereals
ISSN01407791
PublisherWiley
Grant IDF/00 795/B
P61/R18283

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