Water supply and not nitrate concentration determines primary root growth in Arabidopsis

A - Papers appearing in refereed journals

Chapman, N., Whalley, W. R., Lindsey, K. and Miller, A. J. 2011. Water supply and not nitrate concentration determines primary root growth in Arabidopsis. Plant, Cell & Environment. 34 (10), pp. 1630-1638.

AuthorsChapman, N., Whalley, W. R., Lindsey, K. and Miller, A. J.
Abstract

Understanding how root system architecture (RSA) adapts to changing nitrogen and water availability is important for improving acquisition. A sand rhizotron system was developed to study RSA in a porous substrate under tightly regulated nutrient supply. The RSA of Arabidopsis seedlings under differing nitrate (NO(3)(-)) and water supplies in agar and sand was described. The hydraulic conductivity of the root environment was manipulated by using altered sand particle size and matric potentials. Ion-selective microelectrodes were used to quantify NO(3)(-) at the surface of growing primary roots in sands of different particle sizes. Differences in RSA were observed between seedlings grown on agar and sand, and the influence of NO(3)(-) (0.1-10.0 mM) and water on RSA was determined. Primary root length (PRL) was a function of water flux and independent of NO(3)(-). The percentage of roots with laterals correlated with water flux, whereas NO(3)(-) supply was important for basal root (BR) growth. In agar and sand, the NO(3)(-) activities at the root surface were higher than those supplied in the nutrient solution. The sand rhizotron system is a useful tool for the study of RSA, providing a porous growth environment that can be used to simulate the effects of hydraulic conductivity on growth.

KeywordsPlant Sciences
Year of Publication2011
JournalPlant, Cell & Environment
Journal citation34 (10), pp. 1630-1638
Digital Object Identifier (DOI)doi:10.1111/j.1365-3040.2011.02358.x
PubMed ID21707650
Open accessPublished as bronze (free) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeSEF
Publisher's version
PublisherWiley
Wiley-Blackwell
ISSN0140-7791

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