A - Papers appearing in refereed journals
Ponder, K. L., Pritchard, J., Harrington, R. and Bales, J. S. 2000. Difficulties in location and acceptance of phloem sap combined with reduced concentration of phloem amino acids explain lowered performance of the aphid Rhopalosiphum padi on nitrogen deficient barley (Hordeum vulgare ) seedlings. Entomologia Experimentalis et Applicata. 97 (2), pp. 203-210.
|Authors||Ponder, K. L., Pritchard, J., Harrington, R. and Bales, J. S.|
Effects of nitrogen deficiency in hydroponically grown barley seedlings (Hordeum vulgare L.) on the development and reproduction of the aphid Rhopalosiphum padi (L.) (Hemiptera: Aphididae) were investigated. Plant growth was significantly reduced in seedlings grown without nitrogen. Aphid intrinsic rate of increase (r(m)) was also significantly lower on these plants compared with that on plants grown with 8 mol m(-3) nitrogen. Phloem sap was collected from seedling stems by aphid stylectomy and amino acids quantified by HPLC. There was a significant reduction in the concentration of non-essential amino acids as a group, but not of essential amino acids. Electrical penetration graphs (EPG) indicated that aphids reached the phloem more quickly and fed for longer on plants grown with nitrogen. This is the first reported study in which this combination of techniques has been used to understand the interactions of an aphid and plant under different environmental conditions.
|Year of Publication||2000|
|Journal||Entomologia Experimentalis et Applicata|
|Journal citation||97 (2), pp. 203-210|
|Digital Object Identifier (DOI)||doi:10.1046/j.1570-7458.2000.00731.x|
|Open access||Published as non-open access|
|Funder project or code||433|
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