Changes in the proteome of the problem weed blackgrass correlating with multiple-herbicide resistance

A - Papers appearing in refereed journals

Tetard-Jones, C., Sabbadin, F., Moss, S., Hull, R. I., Neve, P. and Edwards, R. 2018. Changes in the proteome of the problem weed blackgrass correlating with multiple-herbicide resistance. The Plant Journal. 94, pp. 709-720.

AuthorsTetard-Jones, C., Sabbadin, F., Moss, S., Hull, R. I., Neve, P. and Edwards, R.
Abstract

Herbicide resistance in grass weeds is now one of the greatest threats to sustainable cereal production in Northern Europe. Multiple-herbicide resistance (MHR), a poorly understood multigenic and quantitative trait, is particularly problematic as it provides tolerance to most classes of chemistries currently used for post-emergence weed control. Using a combination of transcriptomics and proteomics, the evolution of MHR in populations of the weed blackgrass (Alopecurus myosuroides) has been investigated. While over 4500 genes showed perturbation in their expression in MHR versus herbicide sensitive (HS) plants, only a small group of proteins showed >2-fold changes in abundance, with a mere eight proteins consistently associated with this class of resistance. Of the eight, orthologues of three of these proteins are also known to be associated with multiple drug resistance (MDR) in humans, suggesting a cross-phyla conservation in evolved tolerance to chemical agents. Proteomics revealed that MHR could be classified into three sub-types based on the association with resistance to herbicides with differing modes of action (MoA), being either global, specific to diverse chemistries acting on one MoA, or herbicide specific. Furthermore, the proteome of MHR plants were distinct from that of HS plants exposed to a range of biotic (insect feeding, plant–microbe interaction) and abiotic (N-limitation, osmotic, heat, herbicide safening) challenges commonly encountered in the field. It was concluded that MHR in blackgrass is a uniquely evolving trait(s), associated with changes in the proteome that are distinct from responses to conventional plant stresses, but sharing common features with MDR in humans.

Keywordsabiotic and biotic stress safeners; Alopecurus myosuroides; multiple drug resistance; safeners; transcriptomics
Year of Publication2018
JournalThe Plant Journal
Journal citation94, pp. 709-720
Digital Object Identifier (DOI)doi:10.1111/tpj.13892
PubMed ID2957532
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeMultiple Herbicide Resistance in Grass Weeds: from Genes to AgroEcosystems
Publisher's version
Output statusPublished
Publication dates
Online25 Mar 2018
Publication process dates
Accepted21 Feb 2018
PublisherWiley-Blackwell
Copyright licenseCC BY
ISSN0960-7412

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