A - Papers appearing in refereed journals
Ma, Q., Kuzyakov, Y., Pan, W., Tang, S., Chadwick, D. R., Wen, Y., Hill, P. W., Macdonald, A. J., Ge, T., Si, L., Wu, L. and Jones, D. L. 2021. Substrate control of sulphur utilisation and microbial stoichiometry in soil: Results of C-13, N-15, C-14, and S-35 quad labelling. The ISME Journal. https://doi.org/10.1038/s41396-021-00999-7
Authors | Ma, Q., Kuzyakov, Y., Pan, W., Tang, S., Chadwick, D. R., Wen, Y., Hill, P. W., Macdonald, A. J., Ge, T., Si, L., Wu, L. and Jones, D. L. |
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Abstract | Global plant sulphur (S) deficiency is increasing because of a reduction in sulphate-based fertiliser application combined with continuous S withdrawal during harvest. Here, we applied 13C, 15N, 14C, and 35S quad labelling of the S-containing amino acids cysteine (Cys) and methionine (Met) to understand S cycling and microbial S transformations in the soil. The soil microorganisms absorbed the applied Cys and Met within minutes and released SO4 2− within hours. The SO4 2− was reutilised by the MB within days. The initial microbial utilisation and SO4 2− release were determined by amino acid structure. Met released 2.5-fold less SO4 2− than Cys. The microbial biomass retained comparatively more C and S from Met than Cys. The microorganisms decomposed Cys to pyruvate and H2S whereas they converted Met to α-ketobutyrate and S-CH3. The microbial stoichiometries of C, N, and S derived from Cys and Met were balanced after 4 d by Cys-derived SO4 |
Year of Publication | 2021 |
Journal | The ISME Journal |
Digital Object Identifier (DOI) | https://doi.org/10.1038/s41396-021-00999-7 |
Open access | Published as non-open access |
Funder | Biotechnology and Biological Sciences Research Council |
Funder project or code | UK - China Virtual Joint Centre for Improved Nitrogen Agronomy (CINAG) |
The Rothamsted Long Term Experiments [2017-2022] | |
Output status | Published |
Publication dates | |
Online | 11 May 2021 |
Publication process dates | |
Accepted | 26 Apr 2021 |
Publisher | Nature Publishing Group |
ISSN | 1751-7362 |
Permalink - https://repository.rothamsted.ac.uk/item/984qy/substrate-control-of-sulphur-utilisation-and-microbial-stoichiometry-in-soil-results-of-c-13-n-15-c-14-and-s-35-quad-labelling
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