Garlic virus X 11-kDa protein granules move within the cytoplasm and traffic a host protein normally found in the nucleolus

A - Papers appearing in refereed journals

Lu, Y., Yan, F., Guo, W., Zheng, H., Lin, L., Peng, J., Adams, M. J. and Chen, J. 2011. Garlic virus X 11-kDa protein granules move within the cytoplasm and traffic a host protein normally found in the nucleolus. Molecular Plant Pathology. 12 (7), pp. 666-676.

AuthorsLu, Y., Yan, F., Guo, W., Zheng, H., Lin, L., Peng, J., Adams, M. J. and Chen, J.
Abstract

The subcellular localization of the 11-kDa protein (p11) encoded by ORF3 of Garlic virus X (GarVX; genus Allexivirus, family Alphaflexiviridae) was examined by confocal microscopy. Granules with intense fluorescence were visible on the endoplasmic reticulum when p11 fused with green or red fluorescent protein (GFP or RFP) was expressed in epidermal cells of Nicotiana benthamiana. Moreover, the p11-RFP granules moved in the cytoplasm, along the cell periphery and through the cell membranes to adjacent cells. A 17-kDa protein (p17) of garlic interacting with p11 was identified by yeast two-hybridization and bimolecular fluorescence complementation assay. When p17 fused to GFP was expressed in epidermal cells of N. benthamiana, it localized to the nucleolus. However, in the presence of GarVX p11, the distribution of p17 changed to that of p11, but did not appear to affect the pattern of movement of p11.

KeywordsPlant Sciences
Year of Publication2011
JournalMolecular Plant Pathology
Journal citation12 (7), pp. 666-676
Digital Object Identifier (DOI)doi:10.1111/J.1364-3703.2010.00699.X
PubMed ID21726366
Open accessPublished as green open access
FunderBiotechnology and Biological Sciences Research Council
Ministry of Science and Technology of China
State Basic Research Program of China
National Natural Science Foundation of China
Natural Science Foundation of Zhejiang Province
Funder project or codeCentre for Sustainable Pest and Disease Management (PDM)
Characterisation of plant -virus- vector relationships, with particular respect to transmission by plasmodiophorids
Publisher's version
PublisherWiley
Grant ID2010CB126203
30771402
2007DFB30350
Y307169
Y3080417
Y3090148
ISSN1464-6722

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