Ondrej Kosik

NameOndrej Kosik
Job titleResearch Scientist - Plant Biochemistry and Physiology
Email addressondrej.kosik@rothamsted.ac.uk
DepartmentSustainable Soils and Crops
ORCIDhttps://orcid.org/0000-0003-3117-0224
OfficeHarpenden

Research outputs

Challenges to Increasing Dietary Fiber in White Flour and Bread

Shewry, P. R., Prins, A., Kosik, O. and Lovegrove, A. 2024. Challenges to Increasing Dietary Fiber in White Flour and Bread. Journal of Agricultural and Food Chemistry - JAFC. https://doi.org/10.1021/acs.jafc.4c02056

Genetic Approaches to Increase Arabinoxylan and β-Glucan Content in Wheat

Prins, A. and Kosik, O. 2023. Genetic Approaches to Increase Arabinoxylan and β-Glucan Content in Wheat. Plants - Basel. 12 (18), p. 3216. https://doi.org/10.3390/plants12183216

RNAi suppression of xylan synthase genes in wheat starchy endosperm

Wilkinson, M. D., Kosik, O., Halsey, K., Walpole, H., Evans, J., Wood, A. J., Ward, J. L., Mitchell, R. A. C., Lovegrove, A. and Shewry, P. R. 2021. RNAi suppression of xylan synthase genes in wheat starchy endosperm. PLOS ONE. 16 (8), p. e0256350. https://doi.org/10.1371/journal.pone.0256350

Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2

Raffan, S., Sparks, C. A., Huttly, A. K., Hyde, L., Martignago, D., Mead, A., Hanley, S. J., Wilkinson, P., Barker, G., Edwards, K. J., Curtis, T. Y., Usher, S. L., Kosik, O. and Halford, N. G. 2021. Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2 . Plant Biotechnology Journal. 19 (8), pp. 1602-1613. https://doi.org/10.1111/pbi.13573

Diversity of content and composition of cell wall-derived dietary fibre in polished rice

Kosik, O., Romero, M. V., Bandonill, E. H., Abilgos-Ramos, R. G., Sreenivasulu, N., Shewry, P. R. and Lovegrove, A. 2020. Diversity of content and composition of cell wall-derived dietary fibre in polished rice . Journal of Cereal Science. 96, p. 103122. https://doi.org/10.1016/j.jcs.2020.103122

Genetic variation in wheat grain quality is associated with differences in the galactolipid content of flour and the gas bubble properties of dough liquor

Min, B., Salt, L., Wilde, P., Kosik, O., Hassall, K. L., Przewieslik-Allen, A., Burridge, A. J., Poole, M., Snape, J., Wingen, L., Haslam, R. P., Griffiths, S. and Shewry, P. R. 2020. Genetic variation in wheat grain quality is associated with differences in the galactolipid content of flour and the gas bubble properties of dough liquor. Food Chemistry. 60, p. 100093. https://doi.org/10.1016/j.fochx.2020.100093

Loss of TaIRX9b gene function in wheat decreases chain length and amount of arabinoxylan in grain but increases cross-linking

Pellny, T. K., Wood, A., Freeman, J., Halsey, K., Plummer, A., Kosik, O., Shewry, P. R., Lovegrove, A., Phillips, A. L. and Mitchell, R. A. C. 2020. Loss of TaIRX9b gene function in wheat decreases chain length and amount of arabinoxylan in grain but increases cross-linking. Plant Biotechnology Journal. pp. 1-12. https://doi.org/10.1111/pbi.13393

Wheat Cell Wall Polysaccharides (Dietary Fibre)

Shewry, P. R., Kosik, O., Pellny, T. K. and Lovegrove, A. 2020. Wheat Cell Wall Polysaccharides (Dietary Fibre). in: Igrejas, G., Ikeda, T. M. and Guzman, C. (ed.) Wheat Quality For Improving Processing And Human Health Switzerland Springer. pp. 255-272

Identification of a major QTL and associated molecular marker for high arabinoxylan fibre in white wheat flour

Lovegrove, A., Wingen, L. U., Plummer, A., Wood, A., Passmore, D., Kosik, O., Freeman, J., Mitchell, R. A. C., Hassall, K. L., Ulker, M., Tremmel-Bede, K., Rakszegi, M., Bedo, Z., Perretant, M-R., Charmet, G., Pont, C., Salse, J., Leverington Waite, M., Orford, S., Burridge, A., Pellny, T. K., Shewry, P. R. and Griffiths, S. 2020. Identification of a major QTL and associated molecular marker for high arabinoxylan fibre in white wheat flour. PLOS ONE. 15 (2), p. e0227826. https://doi.org/10.1371/journal.pone.0227826

Balancing the double‐edged sword effect of increased resistant starch content and its impact on rice texture: its genetics and molecular physiological mechanisms

Parween, S., Anonuevo, J. J., Butardo Jr., V. M., Misra, G., Anacleto, R., Llorente, C., Kosik, O., Romero, M. V, Bandonill, E. H., Mendioro, M. S., Lovegrove, A., Fernie, A. R., Brotman, Y and Sreenivasulu, N. 2020. Balancing the double‐edged sword effect of increased resistant starch content and its impact on rice texture: its genetics and molecular physiological mechanisms. Plant Biotechnology Journal. pp. 1-15. https://doi.org/10.1111/pbi.13339

Comparative prebiotic activity of mixtures of cereal grain polysaccharides

Harris, S., Monteagudo‑Mera, A., Kosik, O., Charalampopoulos, D., Shewry, P. R. and Lovegrove, A. 2019. Comparative prebiotic activity of mixtures of cereal grain polysaccharides . AMB Express. 9 (1), p. 203. https://doi.org/10.1186/s13568-019-0925-z

Improving Rice Dietary Fibre Content and Consumption for Human Health

Lovegrove, A., Kosik, O., Bandonill, E., Abilgos-Ramos, R., Romero, M., Sreenivasulu, N. and Shewry, P. R. 2019. Improving Rice Dietary Fibre Content and Consumption for Human Health. Journal of Nutritional Science and Vitaminology. 65, pp. S48-S50. https://doi.org/10.3177/jnsv.65.S48

Exploring the role of cereal dietary fiber in digestion

Gouseti, O., Lovegrove, A., Kosik, O., Fryer, P. J., Mills, E. C. N., Gates, F. K., Tucker, G., Latty, C., Shewry, P. R. and Bakalis, S. 2019. Exploring the role of cereal dietary fiber in digestion. Journal of Agricultural and Food Chemistry - JAFC. 67 (30), pp. 8419-8424. https://doi.org/10.1021/acs.jafc.9b01847

Modified expression of ZmMYB167 in Brachypodium distachyon and Zea mays leads to increased cell wall lignin and phenolic content

Bhatia, R., Dalton, S., Roberts, L. A., Moron-Garcia, O. M., Iancono, R., Kosik, O., Gallagher, J. A. and Bosch, M. 2019. Modified expression of ZmMYB167 in Brachypodium distachyon and Zea mays leads to increased cell wall lignin and phenolic content. Scientific Reports. 9 (8800), p. 12pp. https://doi.org/10.1038/s41598-019-45225-9

Determination of the prebiotic activity of wheat arabinogalactan peptide (AGP) using batch culture fermentation

Harris, S., Powers, S. J., Monteagudo-Mera, A., Kosik, O., Lovegrove, A., Shewry, P. R. and Charalampopoulos, D. 2019. Determination of the prebiotic activity of wheat arabinogalactan peptide (AGP) using batch culture fermentation. European Journal Of Nutrition. https://doi.org/10.1007/s00394-019-01908-7

Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota

Kristek, A., Wiese, M., Heuer, P., Kosik, O., Schar, M. Y., Soycan, G., Alsharif, S., Kuhnle, G. G. C., Walton, G. and Spencer, J. P. E. 2019. Oat bran, but not its isolated bioactive β-glucans or polyphenols, have a bifidogenic effect in an in vitro fermentation model of the gut microbiota. British Journal Of Nutrition. 121 (5), pp. 549-559. https://doi.org/10.1017/S0007114518003501

Evaluation of the prebiotic potential of arabinoxylans extracted from wheat distillers’ dried grains with solubles (DDGS) and in-process samples

Monteagudo-Mera, A., Chatzifragkou, A, Kosik, O., Gibson, G., Lovegrove, A., Shewry, P. R. and Charalampopoulos, D. 2018. Evaluation of the prebiotic potential of arabinoxylans extracted from wheat distillers’ dried grains with solubles (DDGS) and in-process samples. Applied Microbiology and Biotechnology. 102 (17), pp. 7577-7587. https://doi.org/10.1007/s00253-018-9171-6

Development and characterisation of protein films derived from dried distillers’ grains with solubles and in-process samples

Prabhakumari, P., Chatzifragkou, A., Kosik, O., Lovegrove, A., Shewry, P. R. and Charalampopoulos, D 2018. Development and characterisation of protein films derived from dried distillers’ grains with solubles and in-process samples. Industrial Crops and Products. 121 (1 October), pp. 258-266. https://doi.org/10.1016/j.indcrop.2018.05.016

Exploiting natural and induced variation to improve the content and composition of dietary fibre in wheat grain

Lovegrove, A., Plummer, A., Wood, A., Passmore, D., Kosik, O., Reynolds, S., Freeman, J., Pellny, T. K., Mitchell, R. A. C., Ulker, M., Rakszegi, M., Bedo, Z, Perretant, M. R., Charmete, G, Wingen, L., Leverington, M., Mondal, S., Singh, R., Guzman, C., Griffiths, S. and Shewry, P. R. 2018. Exploiting natural and induced variation to improve the content and composition of dietary fibre in wheat grain. 13th International Gluten Workshop. CIMMYT, Mexico 14 Mar 2018

Data set of enzyme fingerprinting of dietary fibre components (arabinoxylan and β-glucan) in old and modern Italian durum wheat genotypes

De Santis, M. A., Kosik, O., Passmore, D., Flagella, Z., Shewry, P. R. and Lovegrove, A. 2018. Data set of enzyme fingerprinting of dietary fibre components (arabinoxylan and β-glucan) in old and modern Italian durum wheat genotypes. Data in Brief. 16 (February), pp. 1062-1068. https://doi.org/10.1016/j.dib.2017.12.029

Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp. durum) genotypes

De Santis, M. A., Kosik, O., Passmore, D., Flagella, Z., Shewry, P. R. and Lovegrove, A. 2018. Comparison of the dietary fibre composition of old and modern durum wheat (Triticum turgidum spp. durum) genotypes. Food Chemistry. 244, pp. 304-310. https://doi.org/10.1016/j.foodchem.2017.09.143

In vitro fermentability of xylo-oligosaccharide and xylo-polysaccharide fractions with different molecular weights by human faecal bacteria

Ho, A. L., Kosik, O., Lovegrove, A., Charalampopoulos, D and Rastall, R. A. 2018. In vitro fermentability of xylo-oligosaccharide and xylo-polysaccharide fractions with different molecular weights by human faecal bacteria. Carbohydrate Polymers. 190, pp. 50-58. https://doi.org/10.1016/j.carbpol.2017.08.077

Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNAi lines with suppression of genes responsible for backbone synthesis and decoration

Freeman, J., Ward, J. L., Kosik, O., Lovegrove, A., Wilkinson, M. D., Shewry, P. R. and Mitchell, R. A. C. 2017. Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNAi lines with suppression of genes responsible for backbone synthesis and decoration. Plant Biotechnology Journal. 15 (11), pp. 1429-1438. https://doi.org/10.1111/pbi.12727

Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter

Zielke, C., Kosik, O., Ainalem, M. L., Lovegrove, A., Stradner, A. and Nilsson, L. 2017. Characterization of cereal β-glucan extracts from oat and barley and quantification of proteinaceous matter. PLOS ONE. 12 (2), p. e0172034. https://doi.org/10.1371/journal.pone.0172034

Changes in the arabinoxylan fraction of wheat grain during alcohol production

Kosik, O., Powers, S. J., Chatzifragkou, A., Prabhakumari, P. C., Charalampopoulos, D., Hess, L., Brosnan, J., Shewry, P. R. and Lovegrove, A. 2017. Changes in the arabinoxylan fraction of wheat grain during alcohol production. Food Chemistry. 221 (15 April), pp. 1754-1762. https://doi.org/10.1016/j.foodchem.2016.10.109

Extractability and characteristics of proteins deriving from wheat DDGS

Chatzifragkou, A., Prabhakumari, P. C., Kosik, O., Lovegrove, A., Shewry, P. R. and Charalampopoulos, D. 2016. Extractability and characteristics of proteins deriving from wheat DDGS. Food Chemistry. 198 (1 May), pp. 12-19. https://doi.org/10.1016/j.foodchem.2015.11.036

Biorefinery strategies for upgrading Distillers' Dried Grains with Solubles (DDGS)

Chatzifragkou, A., Kosik, O., Prabhakumari, P. C., Lovegrove, A., Frazier, R. A., Shewry, P. R. and Charalampopoulos, D. 2015. Biorefinery strategies for upgrading Distillers' Dried Grains with Solubles (DDGS). Process Biochemistry. 50 (12), pp. 2194-2207. https://doi.org/10.1016/j.procbio.2015.09.005

Improving wheat as a source of dietary fibre for human health

Shewry, P. R., Reynolds, S., Pellny, T. K., Freeman, J., Wilkinson, M. D., Kosik, O., Ulker, M., Wingen, L., Orford, S., Griffiths, S., Mitchell, R. A. C. and Lovegrove, A. 2015. Improving wheat as a source of dietary fibre for human health. Cambridge University Press (CUP) Cambridge. https://doi.org/10.1017/S0029665115001020

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