PS: Plant Transformation and Genome Editing

TitlePS: Plant Transformation and Genome Editing
TypeResearch cluster

Outputs

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Dough properties of transgenic wheat transformed with Cys-mutated low-molecular-weight glutenin genes

C1 - Edited contributions to conferences/learned societies
Funatsuki, W., Tosi, P., Ito, M., Jones, H. D., Sparks, C. A., Riley, A., Nishio, Z., Tadashi, T., Yamauchi, H. and Shewry, P. R. 2010. Dough properties of transgenic wheat transformed with Cys-mutated low-molecular-weight glutenin genes. Branlard, G. (ed.) Gluten Proteins 2009: Proceedings of the 10th International Gluten Workshop, Clermont-Ferrand, 7-9 September 2009 . Institut National de la Recherche Agronomique (INRA), Paris. pp. 175-179

Increasing the amylose content of durum wheat through silencing of the SBE11a genes

A - Papers appearing in refereed journals
Sestili, F., Janni, M., Doherty, A., Botticella, E., D'ovidio, R., Masci, S., Jones, H. D. and Lafiandra, D. 2010. Increasing the amylose content of durum wheat through silencing of the SBE11a genes. BMC Plant Biology. 10, p. 144 (12pp). https://doi.org/10.1186/1471-2229-10-144

Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat

A - Papers appearing in refereed journals
Nemeth, C., Freeman, J., Jones, H. D., Sparks, C. A., Pellny, T. K., Wilkinson, M. D., Dunwell, J., Andersson, A. A. M., Aman, P., Guillon, F., Saulnier, L., Mitchell, R. A. C. and Shewry, P. R. 2010. Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat. Plant Physiology. 152, pp. 1209-1218. https://doi.org/10.1104/pp.109.151712

Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging

C2 - Non-edited contributions to conferences
Tosi, P., Gritsch, C., Freeman, J., Sparks, C. A., Jones, H. D., Funatsuki, W., Niwa, K. and Shewry, P. R. 2009. Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging. Abstracts Xth International Gluten Workshop, Clermont Ferrand, 7-9 September 2009 .

Stable transformation of plants

B - Book chapters etc edited externally
Jones, H. D. and Sparks, C. A. 2009. Stable transformation of plants. in: Gustafson, J. P., Somers, D. J. and Langridge, P. (ed.) Plant genomics. (Methods in Molecular Biology vol 513) Humana Press, Totowa, New Jersey. pp. 111-130

Transient transformation of plants

B - Book chapters etc edited externally
Jones, H. D., Doherty, A. and Sparks, C. A. 2009. Transient transformation of plants. in: Gustafson, J. P., Somers, D. J. and Langridge, P. (ed.) Plant genomics. (Methods in Molecular Biology vol 513) Humana Press, Totowa, New Jersey. pp. 131-152

Transgenic manipulation of wheat quality

B - Book chapters etc edited externally
Jones, H. D. and Sparks, C. A. 2009. Transgenic manipulation of wheat quality. in: Shewry, P. R. and Khan, K. (ed.) Wheat: chemistry and technology, 4th ed American Association of Cereal Chemists (AACC), St Paul, MN. pp. 437-451

A transgenic approach to understanding gene expression in cereals

C1 - Edited contributions to conferences/learned societies
Jones, H. D., Freeman, J. and Sparks, C. A. 2008. A transgenic approach to understanding gene expression in cereals. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 427-430

Agrobacterium- mediated transformation of bread and durum wheat using freshly isolated immature embryos

B - Book chapters etc edited externally
Wu, H. and Doherty, A. 2008. Agrobacterium- mediated transformation of bread and durum wheat using freshly isolated immature embryos. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 93-103

Biolistics transformation of wheat

B - Book chapters etc edited externally
Sparks, C. A. 2008. Biolistics transformation of wheat. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 71-92

Selection of transformed plants

B - Book chapters etc edited externally
Sparks, C. A. 2008. Selection of transformed plants. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 23-37

Promoter sequences for defining transgene expression

B - Book chapters etc edited externally
Sparks, C. A. 2008. Promoter sequences for defining transgene expression. in: Shewry, P. R. and Jones, H. D. (ed.) In %Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 171-184

Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development

A - Papers appearing in refereed journals
Van Herpen, T. W. J. M., Riley, M., Sparks, C. A., Jones, H. D., Gritsch, C., Dekking, E. H., Hamer, R. J., Bosch, D., Salentijn, E. M. J., Smulders, M. J. M., Shewry, P. R. and Gilissen, L. J. W. J. 2008. Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development. Annals of Botany. 102, pp. 331-342. https://doi.org/10.1093/aob/mcn114

Efficient and rapid Agrobacterium -mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes

A - Papers appearing in refereed journals
Wu, H., Doherty, A. and Jones, H. D. 2008. Efficient and rapid Agrobacterium -mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes. Transgenic Research. 17, pp. 425-436. https://doi.org/10.1007/s11248-007-9116-9

The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations

A - Papers appearing in refereed journals
Pastori, G. M., Huttly, A. K., West, J., Sparks, C. A., Pieters, A., Luna, C. M., Jones, H. D. and Foyer, C. H. 2007. The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations. Functional Plant Biology. 34 (9), pp. 835-843. https://doi.org/10.1071/FP07112

High throughput Agrobacterium transformation of wheat: a tool for functional genomics

C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wilkinson, M. D., Doherty, A. and Wu, H. 2007. High throughput Agrobacterium transformation of wheat: a tool for functional genomics. Buck, H. T., Salomon, N. and Nisi, J. E. (ed.) Wheat Production in Stressed Environments: Proceedings 7th International Wheat Conference, Mar del Plata, Argentina, 27 November-2 December 2005 . Springer, Dordrecht. pp. 693-699

Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength

C2 - Non-edited contributions to conferences
Maruyama-Funatsuki, W., Tosi, P., Jones, H. D., Sparks, C. A., Riley, A., Yamauchi, H. and Shewry, P. R. 2007. Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength.

Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins

C1 - Edited contributions to conferences/learned societies
Shewry, P. R., Tosi, P., Jones, H. D., Sparks, C. A., Gritsch, C., Funatsuki, W., Niwa, K., Huttly, A. K., Napier, J. A., D'ovidio, R. and Freeman, J. 2007. Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins. Lookhart, G. L. and Ng, P. W. K. (ed.) Gluten proteins 2006 . American Association of Cereal Chemists (AACC), St Paul, MN. pp. 112-116

Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium -mediated transformation

A - Papers appearing in refereed journals
Wu, H., Sparks, C. A. and Jones, H. D. 2006. Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium -mediated transformation. Molecular Breeding. 18, pp. 195-208. https://doi.org/10.1007/s11032-006-9027-0

Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat

A - Papers appearing in refereed journals
Jones, H. D., Doherty, A. and Wu, H. 2005. Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat. Plant Methods. 1:5. https://doi.org/10.1186/1746-4811-1-5

Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with the reporter gene construct pAHC25

A - Papers appearing in refereed journals
Howarth, J. R., Jacquet, J. N., Doherty, A., Jones, H. D. and Cannell, M. E. 2005. Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with the reporter gene construct pAHC25. Annals of Applied Biology - AAB. 146 (3), pp. 311-320. https://doi.org/10.1111/j.1744-7348.2005.040121.x

Transformation of wheat by biolistics

B - Book chapters etc edited externally
Sparks, C. A. and Jones, H. D. 2004. Transformation of wheat by biolistics. in: Curtis, I. S. (ed.) Transgenic crops of the world: essential protocols Kluwer Academic Publishers, Dordrecht, Netherlands. pp. 19-33

Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)

A - Papers appearing in refereed journals
Mendoza De Gyves, E., Sparks, C. A., Sayanova, O. V., Lazzeri, P. A., Napier, J. A. and Jones, H. D. 2004. Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.). Plant Biotechnology Journal. 2 (4), pp. 351-357. https://doi.org/10.1111/j.1467-7652.2004.00079.x

The potential of biotechnology to produce novel gluten phenotypes

C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Sparks, C. A., Doherty, A., Cannell, M., Goodwin, J., Pastori, G. M. and Shewry, P. R. 2004. The potential of biotechnology to produce novel gluten phenotypes. D'ovidio, R., Lafiandra, D. and Masci, S. (ed.) Gluten Proteins. Proceedings of the 8th Gluten Workshop, Viterbo, 8-10 September 2003 . Royal Society of Chemistry (RSC), Cambridge. pp. 14-17

Use of MAR sequences to stabilise the expression of a HMW glutenin subunit gene in transgenic wheat

C2 - Non-edited contributions to conferences
Doherty, A., West, J. M., Sparks, C. A., Stone, M. C., Cannell, M. and Jones, H. D. 2003. Use of MAR sequences to stabilise the expression of a HMW glutenin subunit gene in transgenic wheat. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 .

Transgenic approaches to study quality traits in cereals

C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Sparks, C. A. and Shewry, P. R. 2003. Transgenic approaches to study quality traits in cereals. Proceedings BCPC International Congress - Crop Science and Technology, Glasgow, 10-12 November 2003 . pp. 133-140

Promoters for driving transgene expression in wheat

C1 - Edited contributions to conferences/learned societies
Sparks, C. A., Collins, R. and Jones, H. D. 2003. Promoters for driving transgene expression in wheat. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 .

Optimisation factors for Agrobacterium -mediated genetic transformation of wheat (cv Florida)

C1 - Edited contributions to conferences/learned societies
Wu, H., Sparks, C. A. and Jones, H. D. 2003. Optimisation factors for Agrobacterium -mediated genetic transformation of wheat (cv Florida). Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 . pp. 181-184

Agrobacterium mediated transformation of wheat: progress and application

C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Patil, M. S., Rubio, S. and Sparks, C. A. 2003. Agrobacterium mediated transformation of wheat: progress and application. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 . pp. 914-916

Factors influencing successful Agrobacterium -mediated genetic transformation of wheat

A - Papers appearing in refereed journals
Wu, H., Sparks, C. A., Amoah, B. and Jones, H. D. 2003. Factors influencing successful Agrobacterium -mediated genetic transformation of wheat. Plant Cell Reports. 21, pp. 659-668.

Age-dependent transformation frequency in elite wheat varieties

A - Papers appearing in refereed journals
Pastori, G. M., Wilkinson, M. D., Steele, S. H., Sparks, C. A., Jones, H. D. and Parry, M. A. J. 2001. Age-dependent transformation frequency in elite wheat varieties. Journal of Experimental Botany. 52, pp. 857-863.

High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron

A - Papers appearing in refereed journals
Mendoza De Gyves, E., Sparks, C. A., Fieldsend, A. F., Lazzeri, P. A. and Jones, H. D. 2001. High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron. Annals of Applied Biology - AAB. 138 (3), pp. 329-332.

Factors influencing Agrobacterium -mediated transient expression of uid A in wheat inflorescence tissue

A - Papers appearing in refereed journals
Amoah, B. K., Wu, H., Sparks, C. A. and Jones, H. D. 2001. Factors influencing Agrobacterium -mediated transient expression of uid A in wheat inflorescence tissue. Journal of Experimental Botany. 52, pp. 1135-1142.

Potential for manipulating carbon metabolism in wheat

A - Papers appearing in refereed journals
Sparks, C. A., Castleden, C. K., West, J., Habash, D. Z., Madgwick, P. J., Paul, M. J., Noctor, G., Harrison, J., Wu, R., Wilkinson, J., Quick, W. P., Parry, M. A. J., Foyer, C. H. and Miflin, B. J. 2001. Potential for manipulating carbon metabolism in wheat. Annals of Applied Biology - AAB. 138 (1), pp. 33-45. https://doi.org/10.1111/j.1744-7348.2001.tb00083.x

Overexpression of sucrose-phosphate synthase in wheat

C2 - Non-edited contributions to conferences
Castleden, C. K., Sparks, C. A., West, J. M., Quick, W. P. and Foyer, C. H. 1999. Overexpression of sucrose-phosphate synthase in wheat. Abstracts International Conference on Assimilate Transport and Partitioning, Newcastle NSW, 15-20 August 1999 . pp. 95

A population of wheat and tritordeum transformants showing a high degree of marker gene stability and heritability

A - Papers appearing in refereed journals
Cannell, M. E., Doherty, A., Lazzeri, P. A. and Barcelo, P. 1999. A population of wheat and tritordeum transformants showing a high degree of marker gene stability and heritability. Theoretical and Applied Genetics. 99, pp. 772-784.

Current status of transformationin bread and durum wheats and modifications of gluten quality

C1 - Edited contributions to conferences/learned societies
He, G. Y., Rooke, L., Cannell, M., Rasco-Gaunt, S., Sparks, C. A., Lamacchia, C., Bekes, F., Tatham, A. S., Barcelo, P., Shewry, P. R. and Lazzeri, P. A. 1998. Current status of transformationin bread and durum wheats and modifications of gluten quality.

Transient expression of CAT in wheat protoplasts.

G - Articles in popular magazines and other technical publications
Jones, M. G. K., De Both, M. T. J., Wu, Q. S., Sparks, C. A., Maddock, S. E. and Gleddie, S. 1986. Transient expression of CAT in wheat protoplasts.

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