PS: Plant Transformation and Genome Editing
| Title | PS: Plant Transformation and Genome Editing |
|---|---|
| Type | Research cluster |
Outputs
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Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat
A - Papers appearing in refereed journals
Lobo, A. K. M., Orr, D., Gutierrez, M. O., Andralojc, P. J., Sparks, C. A., Afan, M., Parry, J., Parry, M. A. J. and Carmo-Silva, E. 2019. Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat. Plant Physiology. 181 (October), pp. 471-479. https://doi.org/10.1104/pp.19.00693
Overexpression of sucrose-phosphate synthase in wheat
C2 - Non-edited contributions to conferences
Castleden, C. K., Sparks, C. A., West, J. M., Quick, W. P. and Foyer, C. H. 1999. Overexpression of sucrose-phosphate synthase in wheat. Abstracts International Conference on Assimilate Transport and Partitioning, Newcastle NSW, 15-20 August 1999 . pp. 95
Plant U-box E3 ligases PUB20 and PUB21 negatively regulate pattern-triggered immunity in Arabidopsis
A - Papers appearing in refereed journals
Yi, S.Y., Nekrasov, V., Ichimura, K., Kang, S.Y. and Shirasu, K. 2024. Plant U-box E3 ligases PUB20 and PUB21 negatively regulate pattern-triggered immunity in Arabidopsis. Plant Molecular Biology. 114 (7), pp. 1-14. https://doi.org/10.1007/s11103-023-01409-6
Plastidial acyl carrier protein Δ9-desaturase modulates eicosapentaenoic acid biosynthesis and triacylglycerol accumulation in Phaeodactylum tricornutum
A - Papers appearing in refereed journals
Smith, R., Jouhet, J., Gandini, C., Nekrasov, V., Marechal, E., Napier, J. A. and Sayanova, O. V. 2021. Plastidial acyl carrier protein Δ9-desaturase modulates eicosapentaenoic acid biosynthesis and triacylglycerol accumulation in Phaeodactylum tricornutum. Plant Journal. 106 (5), pp. 1247-1259. https://doi.org/10.1111/tpj.15231
Potential for manipulating carbon metabolism in wheat
A - Papers appearing in refereed journals
Sparks, C. A., Castleden, C. K., West, J., Habash, D. Z., Madgwick, P. J., Paul, M. J., Noctor, G., Harrison, J., Wu, R., Wilkinson, J., Quick, W. P., Parry, M. A. J., Foyer, C. H. and Miflin, B. J. 2001. Potential for manipulating carbon metabolism in wheat. Annals of Applied Biology - AAB. 138 (1), pp. 33-45. https://doi.org/10.1111/j.1744-7348.2001.tb00083.x
Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength
C2 - Non-edited contributions to conferences
Maruyama-Funatsuki, W., Tosi, P., Jones, H. D., Sparks, C. A., Riley, A., Yamauchi, H. and Shewry, P. R. 2007. Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength.
Promoter sequences for defining transgene expression
B - Book chapters etc edited externally
Sparks, C. A. 2008. Promoter sequences for defining transgene expression. in: Shewry, P. R. and Jones, H. D. (ed.) In %Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 171-184
Promoters for driving transgene expression in wheat
C1 - Edited contributions to conferences/learned societies
Sparks, C. A., Collins, R. and Jones, H. D. 2003. Promoters for driving transgene expression in wheat. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 .
Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion
A - Papers appearing in refereed journals
Nekrasov, V., Wang, C., Win, J., Lanz, C., Weigel, D. and Kamoun, S. 2017. Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Scientific Reports. 7 (1), p. 482. https://doi.org/10.1038/s41598-017-00578-x
Remarkable recent changes in genetic diversity of the avirulence gene AvrStb6 in global populations of the wheat pathogen Zymoseptoria tritici
A - Papers appearing in refereed journals
Stephens, C., Olmez, F., Blyth, H., McDonald, M., Bansal, A., Turgay, E. B., Hahn, F., Saintenac, C., Nekrasov, V., Solomon, P., Milgate, A., Fraaije, B. A., Rudd, J. J. and Kanyuka, K. 2021. Remarkable recent changes in genetic diversity of the avirulence gene AvrStb6 in global populations of the wheat pathogen Zymoseptoria tritici. Molecular Plant Pathology. 22 (9), pp. 1121-1133. https://doi.org/10.1111/mpp.13101
Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat
A - Papers appearing in refereed journals
Jones, H. D., Doherty, A. and Wu, H. 2005. Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat. Plant Methods. 1:5. https://doi.org/10.1186/1746-4811-1-5
Selection of transformed plants
B - Book chapters etc edited externally
Sparks, C. A. 2008. Selection of transformed plants. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 23-37
Sequence-specific nucleases as tools for enhancing disease resistance in crops
A - Papers appearing in refereed journals
Nekrasov, V. 2019. Sequence-specific nucleases as tools for enhancing disease resistance in crops. Transgenic Research. 28 (Supplement 2), pp. 75-80. https://doi.org/10.1007/s11248-019-00137-2
Silencing an essential gene involved in infestation and digestion in grain aphid through plant‐mediated RNA interference generates aphid‐resistant wheat plants
A - Papers appearing in refereed journals
Sun, Y., Sparks, C. A., Jones, H., Riley, M., Francis, F., Du, W. and Xia, L. 2019. Silencing an essential gene involved in infestation and digestion in grain aphid through plant‐mediated RNA interference generates aphid‐resistant wheat plants. Plant Biotechnology Journal. 17 (5), pp. 852-854. https://doi.org/10.1111/pbi.13067
Stable transformation of plants
B - Book chapters etc edited externally
Jones, H. D. and Sparks, C. A. 2009. Stable transformation of plants. in: Gustafson, J. P., Somers, D. J. and Langridge, P. (ed.) Plant genomics. (Methods in Molecular Biology vol 513) Humana Press, Totowa, New Jersey. pp. 111-130
Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging
C1 - Edited contributions to conferences/learned societies
Tosi, P., Gritsch, C., Freeman, J., Sparks, C. A., Jones, H. D., Funatsuki, W., Niwa, K. and Shewry, P. R. 2010. Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging. Branlard, G. (ed.) Gluten Proteins 2009: Proceedings 10th International Gluten Workshop, Clermont-Ferrand, 7-9 September 2009 . Institut National de la Recherche Agronomique (INRA), Paris. pp. 17-20
Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging
C2 - Non-edited contributions to conferences
Tosi, P., Gritsch, C., Freeman, J., Sparks, C. A., Jones, H. D., Funatsuki, W., Niwa, K. and Shewry, P. R. 2009. Studying the pattern of synthesis, deposition and tissue location of gluten proteins by epitope tagging. Abstracts Xth International Gluten Workshop, Clermont Ferrand, 7-9 September 2009 .
Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat
A - Papers appearing in refereed journals
Freeman, J., Sparks, C. A., West, J., Shewry, P. R. and Jones, H. D. 2011. Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat. Plant Biotechnology Journal. 9 (7), pp. 788-796. https://doi.org/10.1111/j.1467-7652.2011.00588.x
Temporal and spatial transgene expression using a heat-shock promoter in transgenic wheat
C1 - Edited contributions to conferences/learned societies
Freeman, J., Sparks, C. A., West, J. S., Shewry, P. R. and Jones, H. D. 2011. Temporal and spatial transgene expression using a heat-shock promoter in transgenic wheat. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 85-91
The Arabidopsis pattern recognition receptor EFR enhances fire blight resistance in apple
A - Papers appearing in refereed journals
Piazza, S., Campa, M., Pompili, V., Dalla Costa, L., Salvagnin, U., Nekrasov, V., Zipfel, C. and Malnoy, M. 2021. The Arabidopsis pattern recognition receptor EFR enhances fire blight resistance in apple. Horticulture Research. 8, p. 204. https://doi.org/10.1038/s41438-021-00639-3
The first crop plant genetically engineered to release an insect pheromone for defence
A - Papers appearing in refereed journals
Bruce, T. J. A., Aradottir, G. I., Smart, L. E., Martin, J. L., Caulfield, J. C., Doherty, A., Sparks, C. A., Woodcock, C. M., Birkett, M. A., Napier, J. A., Jones, H. D. and Pickett, J. A. 2015. The first crop plant genetically engineered to release an insect pheromone for defence. Scientific Reports. 5, p. 11183. https://doi.org/10.1038/srep11183
The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations
A - Papers appearing in refereed journals
Pastori, G. M., Huttly, A. K., West, J., Sparks, C. A., Pieters, A., Luna, C. M., Jones, H. D. and Foyer, C. H. 2007. The maize Activator/Dissociation system is functional in hexaploid wheat through successive generations. Functional Plant Biology. 34 (9), pp. 835-843. https://doi.org/10.1071/FP07112
The potential of biotechnology to produce novel gluten phenotypes
C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Sparks, C. A., Doherty, A., Cannell, M., Goodwin, J., Pastori, G. M. and Shewry, P. R. 2004. The potential of biotechnology to produce novel gluten phenotypes. D'ovidio, R., Lafiandra, D. and Masci, S. (ed.) Gluten Proteins. Proceedings of the 8th Gluten Workshop, Viterbo, 8-10 September 2003 . Royal Society of Chemistry (RSC), Cambridge. pp. 14-17
Transformation of wheat by biolistics
B - Book chapters etc edited externally
Sparks, C. A. and Jones, H. D. 2004. Transformation of wheat by biolistics. in: Curtis, I. S. (ed.) Transgenic crops of the world: essential protocols Kluwer Academic Publishers, Dordrecht, Netherlands. pp. 19-33
Transgenic approaches to study quality traits in cereals
C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Sparks, C. A. and Shewry, P. R. 2003. Transgenic approaches to study quality traits in cereals. Proceedings BCPC International Congress - Crop Science and Technology, Glasgow, 10-12 November 2003 . pp. 133-140
Transgenic manipulation of wheat quality
B - Book chapters etc edited externally
Jones, H. D. and Sparks, C. A. 2009. Transgenic manipulation of wheat quality. in: Shewry, P. R. and Khan, K. (ed.) Wheat: chemistry and technology, 4th ed American Association of Cereal Chemists (AACC), St Paul, MN. pp. 437-451
Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene
C1 - Edited contributions to conferences/learned societies
Bruce, T. J. A., Smart, L. E., Martin, J. L., Doherty, A., Jones, H. D., Napier, J. A. and Pickett, J. A. 2011. Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 24
Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene
C1 - Edited contributions to conferences/learned societies
Bruce, T. J. A., Smart, L. E., Aradottir, G. I., Martin, J. L., Doherty, A., Jones, H. D., Napier, J. A. and Pickett, J. A. 2011. Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene. GM Crops: from Basic Research to Application. Aspects of Applied Biology 110 . Association of Applied Biologists (AAB) Wellesbourne Warwick . pp. 112
Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene
C2 - Non-edited contributions to conferences
Bruce, T. J. A., Smart, L. E., Aradottir, G. I., Martin, J. L., Doherty, A., Jones, H. D., Napier, J. A. and Pickett, J. A. 2011. Transgenic wheat emitting aphid alarm pheromone, (E)-beta-farnesene. Abstracts Ento' 11 Symposium on Chemical Ecology: Reception, Detection and Deception, Chatham, 7-9 September 2011 . pp. 54
Transient expression of CAT in wheat protoplasts.
G - Articles in popular magazines and other technical publications
Jones, M. G. K., De Both, M. T. J., Wu, Q. S., Sparks, C. A., Maddock, S. E. and Gleddie, S. 1986. Transient expression of CAT in wheat protoplasts.
Transient transformation of plants
B - Book chapters etc edited externally
Jones, H. D., Doherty, A. and Sparks, C. A. 2009. Transient transformation of plants. in: Gustafson, J. P., Somers, D. J. and Langridge, P. (ed.) Plant genomics. (Methods in Molecular Biology vol 513) Humana Press, Totowa, New Jersey. pp. 131-152
Use of a modified CRISPR/Cas9 construct to simultaneously mutagenize multiple genes in hexaploid wheat
C1 - Edited contributions to conferences/learned societies
Winfield, M., Allen, S., Cartlidge, L., Sparks, C. A., Jones, H., Huttly, A. K., Martignago, D., Hawkesford, M. J. and Edwards, K. 2017. Use of a modified CRISPR/Cas9 construct to simultaneously mutagenize multiple genes in hexaploid wheat. MonoGram 2017, 4-6 April, University of Bristol. Bristol, University of Bristol.
Use of MAR sequences to stabilise the expression of a HMW glutenin subunit gene in transgenic wheat
C2 - Non-edited contributions to conferences
Doherty, A., West, J. M., Sparks, C. A., Stone, M. C., Cannell, M. and Jones, H. D. 2003. Use of MAR sequences to stabilise the expression of a HMW glutenin subunit gene in transgenic wheat. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 .
Use of the pre-assembled ribonucleoprotein (RNP) complex for gene editing of wheat and poplar
Conference poster
Sparks, C. A., Cerezo-Medina, S., Raffan, S. and Hahn, F. 2019. Use of the pre-assembled ribonucleoprotein (RNP) complex for gene editing of wheat and poplar. The 7th Plant Genomics and Gene Editing Congress. Rotterdam, The Netherlands 21 May 2019
Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins
C1 - Edited contributions to conferences/learned societies
Shewry, P. R., Tosi, P., Jones, H. D., Sparks, C. A., Gritsch, C., Funatsuki, W., Niwa, K., Huttly, A. K., Napier, J. A., D'ovidio, R. and Freeman, J. 2007. Using epitope tagging to explore the trafficking, location and functional properties of wheat gluten proteins. Lookhart, G. L. and Ng, P. W. K. (ed.) Gluten proteins 2006 . American Association of Cereal Chemists (AACC), St Paul, MN. pp. 112-116
Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain
A - Papers appearing in refereed journals
Wan, Y., Wang, Y., Shi, Z., Rentsch, D., Ward, J. L., Hassall, K. L., Sparks, C. A., Huttly, A. K., Buchner, P. H., Powers, S. J., Shewry, P. R. and Hawkesford, M. J. 2021. Wheat amino acid transporters highly expressed in grain cells regulate amino acid accumulation in grain. PLOS ONE. 16 (2), p. e0246763. https://doi.org/10.1371/journal.pone.0246763
Wheat biotechnology: recent developments and future trends
B - Book chapters etc edited externally
Sparks, C. A. 2016. Wheat biotechnology: recent developments and future trends. in: Jones, H. D. (ed.) Biotechnology of major cereals Chapter 6 CABI International, Wallingford, Oxon (CABI). pp. 88-103
Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2
A - Papers appearing in refereed journals
Raffan, S., Sparks, C. A., Huttly, A. K., Hyde, L., Martignago, D., Mead, A., Hanley, S. J., Wilkinson, P., Barker, G., Edwards, K. J., Curtis, T. Y., Usher, S. L., Kosik, O. and Halford, N. G. 2021. Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2 . Plant Biotechnology Journal. 19 (8), pp. 1602-1613. https://doi.org/10.1111/pbi.13573