PS: Plant Transformation and Genome Editing
| Title | PS: Plant Transformation and Genome Editing |
|---|---|
| Type | Research cluster |
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A high soluble-fibre allele in wheat encodes a defective cell wall peroxidase responsible for dimerization of ferulate moieties on arabinoxylan
A - Papers appearing in refereed journals
Mitchell, R. A. C., Oszvald, M., Pellny, T. K., Freeman, J., Halsey, K., Sparks, C. A., Huttly, A. K., Specel, S., Leverington-Waite, M., Griffiths, S., Shewry, P. R. and Lovegrove, A. 2023. A high soluble-fibre allele in wheat encodes a defective cell wall peroxidase responsible for dimerization of ferulate moieties on arabinoxylan. bioRxiv. https://doi.org/10.1101/2023.03.08.531735
A modified Agrobacterium-mediated transformation for two oomycete pathogens
A - Papers appearing in refereed journals
Wang, L., Zhao, F., Liu, H., Chen, H., Zhang, F., Li, S., Sun, T., Nekrasov, V., Huang, S. and Dong, S. 2023. A modified Agrobacterium-mediated transformation for two oomycete pathogens. PLOS Pathogens. 19 (4), p. e1011346. https://doi.org/10.1371/journal.ppat.1011346
A Modular Cloning Toolkit for Genome Editing in Cereals
B - Book chapters etc edited externally
Hahn, F., Sanjurjo-Loures, L. and Nekrasov, V. 2022. A Modular Cloning Toolkit for Genome Editing in Cereals. in: Bilichak, A. and Laurie, J. D. (ed.) Accelerated Breeding of Cereal Crops New York, NY Humana Press Inc. pp. 209-224
A modular cloning toolkit for genome editing in plants
A - Papers appearing in refereed journals
Hahn, F., Korolev, A., Sanjurjo-Loures, L. and Nekrasov, V. 2020. A modular cloning toolkit for genome editing in plants. BMC Plant Biology. 20, p. 179. https://doi.org/10.1186/s12870-020-02388-2
A population of wheat and tritordeum transformants showing a high degree of marker gene stability and heritability
A - Papers appearing in refereed journals
Cannell, M. E., Doherty, A., Lazzeri, P. A. and Barcelo, P. 1999. A population of wheat and tritordeum transformants showing a high degree of marker gene stability and heritability. Theoretical and Applied Genetics. 99, pp. 772-784.
A transgenic approach to understanding gene expression in cereals
C1 - Edited contributions to conferences/learned societies
Jones, H. D., Freeman, J. and Sparks, C. A. 2008. A transgenic approach to understanding gene expression in cereals. Molinacano, J. L. (ed.) Cereal Science and Technology for Feeding Ten Billion People: Genomics Era and Beyond. Proceedings of the Meeting of the Eucarpia Cereal Section, Lleida (Spain), 13-17 November 2006 . Options Mediterraneennes, Series A, No 81. pp. 427-430
Age-dependent transformation frequency in elite wheat varieties
A - Papers appearing in refereed journals
Pastori, G. M., Wilkinson, M. D., Steele, S. H., Sparks, C. A., Jones, H. D. and Parry, M. A. J. 2001. Age-dependent transformation frequency in elite wheat varieties. Journal of Experimental Botany. 52, pp. 857-863.
Agrobacterium mediated transformation of wheat: progress and application
C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wu, H., Patil, M. S., Rubio, S. and Sparks, C. A. 2003. Agrobacterium mediated transformation of wheat: progress and application. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 . pp. 914-916
Agrobacterium- mediated transformation of bread and durum wheat using freshly isolated immature embryos
B - Book chapters etc edited externally
Wu, H. and Doherty, A. 2008. Agrobacterium- mediated transformation of bread and durum wheat using freshly isolated immature embryos. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 93-103
Biolistics transformation of wheat
B - Book chapters etc edited externally
Sparks, C. A. 2008. Biolistics transformation of wheat. in: Shewry, P. R. and Jones, H. D. (ed.) Transgenic wheat, barley and oats. Methods in molecular biology, vol. 478 Humana Press, New York. pp. 71-92
Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium -mediated transformation
A - Papers appearing in refereed journals
Wu, H., Sparks, C. A. and Jones, H. D. 2006. Characterisation of T-DNA loci and vector backbone sequences in transgenic wheat produced by Agrobacterium -mediated transformation. Molecular Breeding. 18, pp. 195-208. https://doi.org/10.1007/s11032-006-9027-0
Characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici
A - Papers appearing in refereed journals
Kettles, G. J., Bayon, C., Sparks, C. A., Canning, G., Kanyuka, K. and Rudd, J. J. 2018. Characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici. New Phytologist. 217 (1), pp. 320-331. https://doi.org/10.1111/nph.14786
CRISPR-Cas9 multiplex editing of the α-amylase/trypsin inhibitor genes to reduce allergen proteins in durum wheat
A - Papers appearing in refereed journals
Camerlengo, F., Frittelli, A., Sparks, C. A., Doherty, A., Martignago, D., Larre, C., Lupi, R., Sestili, F. and Masci, S. 2020. CRISPR-Cas9 multiplex editing of the α-amylase/trypsin inhibitor genes to reduce allergen proteins in durum wheat. Frontiers in Sustainable Food Systems. https://doi.org/10.3389/fsufs.2020.00104
CRISPR/Cas precision: do we need to worry about off-targeting in plants?
A - Papers appearing in refereed journals
Hahn, F. and Nekrasov, V. 2018. CRISPR/Cas precision: do we need to worry about off-targeting in plants? Plant Cell Reports. https://doi.org/10.1007/s00299-018-2355-9
Current status of transformationin bread and durum wheats and modifications of gluten quality
C1 - Edited contributions to conferences/learned societies
He, G. Y., Rooke, L., Cannell, M., Rasco-Gaunt, S., Sparks, C. A., Lamacchia, C., Bekes, F., Tatham, A. S., Barcelo, P., Shewry, P. R. and Lazzeri, P. A. 1998. Current status of transformationin bread and durum wheats and modifications of gluten quality.
Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development
A - Papers appearing in refereed journals
Van Herpen, T. W. J. M., Riley, M., Sparks, C. A., Jones, H. D., Gritsch, C., Dekking, E. H., Hamer, R. J., Bosch, D., Salentijn, E. M. J., Smulders, M. J. M., Shewry, P. R. and Gilissen, L. J. W. J. 2008. Detailed analysis of the expression of an alpha-gliadin promoter and the deposition of alpha-gliadin protein during wheat grain development. Annals of Botany. 102, pp. 331-342. https://doi.org/10.1093/aob/mcn114
Dough properties of transgenic wheat transformed with Cys-mutated low-molecular-weight glutenin genes
C1 - Edited contributions to conferences/learned societies
Funatsuki, W., Tosi, P., Ito, M., Jones, H. D., Sparks, C. A., Riley, A., Nishio, Z., Tadashi, T., Yamauchi, H. and Shewry, P. R. 2010. Dough properties of transgenic wheat transformed with Cys-mutated low-molecular-weight glutenin genes. Branlard, G. (ed.) Gluten Proteins 2009: Proceedings of the 10th International Gluten Workshop, Clermont-Ferrand, 7-9 September 2009 . Institut National de la Recherche Agronomique (INRA), Paris. pp. 175-179
Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat
A - Papers appearing in refereed journals
Nemeth, C., Freeman, J., Jones, H. D., Sparks, C. A., Pellny, T. K., Wilkinson, M. D., Dunwell, J., Andersson, A. A. M., Aman, P., Guillon, F., Saulnier, L., Mitchell, R. A. C. and Shewry, P. R. 2010. Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat. Plant Physiology. 152, pp. 1209-1218. https://doi.org/10.1104/pp.109.151712
Editorial: CRISPR-Cas in Agriculture: Opportunities and Challenges
A - Papers appearing in refereed journals
Kumar, S., Rymarquis, L. A., Ezura, H. and Nekrasov, V. 2021. Editorial: CRISPR-Cas in Agriculture: Opportunities and Challenges. Frontiers in Plant Science. 12, p. 672329. https://doi.org/10.3389/fpls.2021.672329
Efficient and rapid Agrobacterium -mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes
A - Papers appearing in refereed journals
Wu, H., Doherty, A. and Jones, H. D. 2008. Efficient and rapid Agrobacterium -mediated genetic transformation of durum wheat (Triticum turgidum L. var. durum) using additional virulence genes. Transgenic Research. 17, pp. 425-436. https://doi.org/10.1007/s11248-007-9116-9
Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties
A - Papers appearing in refereed journals
Hahn, F., Sanjurjo-Loures, L., Sparks, C. A., Kanyuka, K. and Nekrasov, V. 2021. Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties. Plants - Basel. 10 (7), p. 1481. https://doi.org/10.3390/plants10071481
Factors influencing Agrobacterium -mediated transient expression of uid A in wheat inflorescence tissue
A - Papers appearing in refereed journals
Amoah, B. K., Wu, H., Sparks, C. A. and Jones, H. D. 2001. Factors influencing Agrobacterium -mediated transient expression of uid A in wheat inflorescence tissue. Journal of Experimental Botany. 52, pp. 1135-1142.
Factors influencing successful Agrobacterium -mediated genetic transformation of wheat
A - Papers appearing in refereed journals
Wu, H., Sparks, C. A., Amoah, B. and Jones, H. D. 2003. Factors influencing successful Agrobacterium -mediated genetic transformation of wheat. Plant Cell Reports. 21, pp. 659-668.
Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.)
A - Papers appearing in refereed journals
Mendoza De Gyves, E., Sparks, C. A., Sayanova, O. V., Lazzeri, P. A., Napier, J. A. and Jones, H. D. 2004. Genetic manipulation of gamma-linolenic acid (GLA) synthesis in a commercial variety of evening primrose (Oenothera sp.). Plant Biotechnology Journal. 2 (4), pp. 351-357. https://doi.org/10.1111/j.1467-7652.2004.00079.x
Genetic transformation of common wheat (Triticum aestivum L.) using biolistics
B - Book chapters etc edited externally
Sparks, C. A. and Doherty, A. 2020. Genetic transformation of common wheat (Triticum aestivum L.) using biolistics. in: Rustgi, S. and Luo, H. (ed.) Biolistic DNA Delivery in Plants New York Humana Press Inc.
Genetic transformation of wheat via Agrobacterium-mediated DNA delivery
B - Book chapters etc edited externally
Sparks, C. A., Doherty, A. and Jones, H. D. 2014. Genetic transformation of wheat via Agrobacterium-mediated DNA delivery. in: Henry, R. J. and Furtado, A. (ed.) Cereal genomics: methods and protocols. Methods in Molecular Biology, vol 1099 Springer, New York. pp. 235-250
Genetic transformation of wheat via particle bombardment
B - Book chapters etc edited externally
Sparks, C. A. and Jones, H. D. 2014. Genetic transformation of wheat via particle bombardment. in: Henry, R. J. and Furtado, A. (ed.) Cereal genomics: methods and protocols. Methods in Molecular Biology, vol 1099 Springer, New York. pp. 201-218
High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron
A - Papers appearing in refereed journals
Mendoza De Gyves, E., Sparks, C. A., Fieldsend, A. F., Lazzeri, P. A. and Jones, H. D. 2001. High frequency of adventitious shoot regeneration from commercial cultivars of evening primrose (Oenothera spp.) using thidiazuron. Annals of Applied Biology - AAB. 138 (3), pp. 329-332.
High throughput Agrobacterium transformation of wheat: a tool for functional genomics
C1 - Edited contributions to conferences/learned societies
Jones, H. D., Wilkinson, M. D., Doherty, A. and Wu, H. 2007. High throughput Agrobacterium transformation of wheat: a tool for functional genomics. Buck, H. T., Salomon, N. and Nisi, J. E. (ed.) Wheat Production in Stressed Environments: Proceedings 7th International Wheat Conference, Mar del Plata, Argentina, 27 November-2 December 2005 . Springer, Dordrecht. pp. 693-699
Host-induced gene silencing for the control of the Fusarium Head Blight in wheat fields
C2 - Non-edited contributions to conferences
Kanyuka, K., Machado, A., Urban, M., Lee, W. S., Brown, N. J., King, R. A., Lau, E. Y., Sparks, C. A., Bonato, A. L. V., Tibola, C. S., Martins, N. F., Aragao, F. J., West, J., Fernandes, J. M. C. and Hammond-Kosack, K. E. 2017. Host-induced gene silencing for the control of the Fusarium Head Blight in wheat fields. 1st COST iPlanta Conferernce; "Creating the plant RNAi research network CA15223", La Sapienza - CNR Headquarter , Rome, Italy, 15-17 February 2017.
Identification of Leaf Promoters for Use in Transgenic Wheat
A - Papers appearing in refereed journals
Alotaibi, S. S., Sparks, C. A., Parry, M. A. J., Simkin, A. J. and Raines, C. A. 2018. Identification of Leaf Promoters for Use in Transgenic Wheat. Plants - Basel. 7 (2), p. 27. https://doi.org/10.3390/plants7020027
Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants
A - Papers appearing in refereed journals
Hyde, L., Osman, K., Winfield, M., Sanchez-Moran, E., Higgins, J. D., Henderson, I. R., Sparks, C. A., Franklin, F. C. H. and Edwards, K. 2022. Identification, characterization, and rescue of CRISPR/Cas9 generated wheat SPO11-1 mutants. Plant Biotechnology Journal. 2022, pp. 1-4. https://doi.org/10.1111/pbi.13961
Improving disease resistance in mungbean - A combined genetic and biotechnological approach
Conference poster
Bansal, A., Nekrasov, V., Rout, G. R., Rudd, J. J., Slavov, G., Chand, R. and Kanyuka, K. 2019. Improving disease resistance in mungbean - A combined genetic and biotechnological approach. BSPP 2019: Arms race: Evolution of plant pathogens and their hosts. Exhibition and Conference Centre, University of West England, Bristol, UK 02 Sep 2019
Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions
A - Papers appearing in refereed journals
Driever, S. M., Simkin, A. J., Alotaibi, S., Fisk, S. J., Madgwick, P. J., Sparks, C. A., Jones, H. D., Lawson, T., Parry, M. A. J. and Raines, C. A. 2017. Increased SBPase activity improves photosynthesis and grain yield in wheat grown in greenhouse conditions. Philosophical Transactions of the Royal Society B-Biological Sciences. 372 (1730), pp. 1-10. https://doi.org/10.1098/rstb.2016.0384
Increasing the amylose content of durum wheat through silencing of the SBE11a genes
A - Papers appearing in refereed journals
Sestili, F., Janni, M., Doherty, A., Botticella, E., D'ovidio, R., Masci, S., Jones, H. D. and Lafiandra, D. 2010. Increasing the amylose content of durum wheat through silencing of the SBE11a genes. BMC Plant Biology. 10, p. 144 (12pp). https://doi.org/10.1186/1471-2229-10-144
Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat
A - Papers appearing in refereed journals
Pearce, S., Saville, R., Vaughan, S. P., Chandler, P. M., Wilhelm, E. P., Sparks, C. A., Al-Kaff, N., Korolev, A., Boulton, M. I., Phillips, A. L., Hedden, P., Nicholson, P. and Thomas, S. G. 2011. Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat. Plant Physiology. 157, pp. 1820-1831. https://doi.org/10.1104/pp.111.183657
Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with the reporter gene construct pAHC25
A - Papers appearing in refereed journals
Howarth, J. R., Jacquet, J. N., Doherty, A., Jones, H. D. and Cannell, M. E. 2005. Molecular genetic analysis of silencing in two lines of Triticum aestivum transformed with the reporter gene construct pAHC25. Annals of Applied Biology - AAB. 146 (3), pp. 311-320. https://doi.org/10.1111/j.1744-7348.2005.040121.x
NRC4 gene cluster is not essential for bacterial flagellin-triggered immunity
A - Papers appearing in refereed journals
Wu, C. H., Adachi, H., De la Concepcion, J. C., Castells-Graells, R., Nekrasov, V. and Kamoun, S. 2019. NRC4 gene cluster is not essential for bacterial flagellin-triggered immunity. Plant Physiology. https://doi.org/10.1104/pp.19.00859
Opportunities for research via wheat transformation
C2 - Non-edited contributions to conferences
Sparks, C. A., Martignago, D., Huttly, A. K., Raffan, S., Doherty, A. and Edwards, K. 2017. Opportunities for research via wheat transformation. The 5th Plant Genomics and Gene Editing Congress, Amsterdam, 16–17 March 2017. Societas Physiologiae Plantarum Scandinavica.
Optimisation factors for Agrobacterium -mediated genetic transformation of wheat (cv Florida)
C1 - Edited contributions to conferences/learned societies
Wu, H., Sparks, C. A. and Jones, H. D. 2003. Optimisation factors for Agrobacterium -mediated genetic transformation of wheat (cv Florida). Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 . pp. 181-184