A - Papers appearing in refereed journals
Karolewski, Z., Fitt, B. D. L., Latunde-Dada, A. O., Foster, S. J., Todd, A. D., Downes, K. and Evans, N. 2006. Visual and PCR assessment of light leaf spot (Pyrenopeziza brassicae) on winter oilseed rape (Brassica napus) cultivars. Plant Pathology. 55 (3), pp. 387-400.
|Authors||Karolewski, Z., Fitt, B. D. L., Latunde-Dada, A. O., Foster, S. J., Todd, A. D., Downes, K. and Evans, N.|
Methods to assess light leaf spot (Pyrenopeziza brassicae) on winter oilseed rape cultivars were compared in laboratory, controlled-environment and field experiments. In controlled-environment experiments with seedling leaves inoculated at GS 1,4, the greatest differences in percentage area affected by P. brassicae sporulation were observed with inoculum concentrations of 4 x 10(3) or 4 x 10(4) spores mL(-1), rather than 4 x 10(2) or 4 x 10(5) spores mL(-1), but older leaves had begun to senesce before assessment, particularly where they were severely affected by P. brassicae. In winter oilseed rape field experiments, a severe light leaf spot epidemic developed in 2002/03 (inoculated, September/October rainfall 127.2 mm) but not in 2003/04 (uninoculated, September/October rainfall 40.7 mm). In-plot assessments discriminated between cultivars best in February/March in 2003 and June in 2004, but sometimes failed to detect plots with many infected plants (e.g. March/April 2004). Ranking of cultivar resistance differed between seedling experiments done under controlled-environment conditions and field experiments. The sensitivity of detection of P. brassicae DNA extracted from culture was greater using the PCR primer pair PbITSF/PbITSR than using primers Pb1/Pb2. P. brassicae was detected by PCR (PbITS primers) in leaves from controlled-environment experiments immediately and up to 14 days after inoculation, and in leaves sampled from field experiments 2 months before detection by visual assessment.
|Keywords||Agronomy; Plant Sciences|
|Year of Publication||2006|
|Journal citation||55 (3), pp. 387-400|
|Digital Object Identifier (DOI)||doi:10.1111/j.1365-3059.2006.01383.x|
|Open access||Published as non-open access|
|Funder project or code||513|
Permalink - https://repository.rothamsted.ac.uk/item/8999x/visual-and-pcr-assessment-of-light-leaf-spot-pyrenopeziza-brassicae-on-winter-oilseed-rape-brassica-napus-cultivars