A - Papers appearing in refereed journals
Kwasna, H. and Bateman, G. L. 2009. Microbial communities in roots of Pinus sylvestris seedlings with damping-off symptoms in two forest nurseries as determined by ITS1/2 rDNA sequencing. Forest Pathology. 39 (4), pp. 239-248.
|Authors||Kwasna, H. and Bateman, G. L.|
P>A methodological molecular procedure, which included extraction and cloning of the ITS1/2 rDNA of root-associated organisms with subsequent transformation and sequencing of representative clones, was effective for detection, discrimination and determination of the frequency of the main damping-off pathogens in roots of Pinus sylvestris seedlings growing in different forest-tree nursery soils and exhibiting different rates of disease progress. Roots exhibiting slower damping-off progression were colonized by Fusarium oxysporum, Neonectria radicicola (Ascomycota) and Pythium spp. (Oomycota), which comprised 50% of the microbial community. Roots exhibiting faster damping-off progression were dominated by Thanatephorus cucumeris (Basidiomycota), which comprised 80% of the microbial community. The microbial community was more diverse in roots with slower damping-off progression (14 species) than in roots with faster disease progression (seven species).
|Year of Publication||2009|
|Journal citation||39 (4), pp. 239-248|
|Digital Object Identifier (DOI)||doi:10.1111/j.1439-0329.2008.00583.x|
|Open access||Published as non-open access|
|Funder project or code||PDM|
|Biotechnology and Biological Sciences Research Council|
Permalink - https://repository.rothamsted.ac.uk/item/8q39w/microbial-communities-in-roots-of-pinus-sylvestris-seedlings-with-damping-off-symptoms-in-two-forest-nurseries-as-determined-by-its1-2-rdna-sequencing