Comparisons of isolates of Fusarium avenaceum from white lupin and other crops by pathogenicity tests, DNA analyses and vegetative compatibility tests

A - Papers appearing in refereed journals

Satyaprasad, K., Bateman, G. L. and Ward, E. 2000. Comparisons of isolates of Fusarium avenaceum from white lupin and other crops by pathogenicity tests, DNA analyses and vegetative compatibility tests. Journal of Phytopathology. 148 (4), pp. 211-219.

AuthorsSatyaprasad, K., Bateman, G. L. and Ward, E.
Abstract

Isolates of Fusarium avenaceum, mostly from crops of white lupin or wheat, were tested for pathogenicity on white lupin and wheat plants and compared by DNA tests and, in a limited study, vegetative compatibility. Most of the 80 isolates were pathogenic on both plant species after inoculation on shoot bases. Disease severity was greater at higher incubation temperatures that ranged from 15/10 degrees C to 25/20 degrees C (day/night temperatures). Isolates from lupin crops tended to be more pathogenic, on average, on lupins than on cereals. Polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis of the internal transcribed spacer region of the rDNA distinguished two groups of isolates that occurred in different proportions among isolates from lupins and cereal crops. Random amplified polymorphic DNA (RAPD)-PCR analyses indicated considerable genetic variation among isolates, but there was some similarity among groups of isolates from populations in the same field. Genetic diversity was confirmed by a high degree of vegetative incompatibility among 20 isolates using nitrate nonutilizing mutants. There were no relationships among pathogenicity, RFLP group, RAPD group and vegetative compatibility group.

KeywordsPlant Sciences
Year of Publication2000
JournalJournal of Phytopathology
Journal citation148 (4), pp. 211-219
Digital Object Identifier (DOI)doi:10.1046/j.1439-0434.2000.00494.x
Open accessPublished as non-open access
Funder project or code204
201
423
442
ISSN09311785
PublisherWiley

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