The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides

A - Papers appearing in refereed journals

Fan, J., Chen, F., Diao, Y., Cools, H. J., Kelly, S. L. and Liu, X. 2014. The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides. Plant Pathology. 63 (4), pp. 952-960. https://doi.org/10.1111/ppa.12168

AuthorsFan, J., Chen, F., Diao, Y., Cools, H. J., Kelly, S. L. and Liu, X.
Abstract

Fusarium verticillioides reduces corn yield and contaminates infected kernels with the toxin fumonisin, which is harmful to humans and animals. Previous research has demonstrated that F. verticillioides can be controlled by the azole fungicide prochloraz. Currently, prochloraz is used as a foliar spray to control maize disease in China, which will increase the risk of resistance. Although F. verticillioides resistance to prochloraz has not been reported in the field, possible resistance risk and mechanisms resulting in prochloraz resistance were explored in the laboratory. Four prochloraz-resistant strains of F. verticillioides were generated by successive selection on fungicide-amended media. The mycelial growth rates of the mutants were inversely related to the level of resistance. All four mutants were cross-resistant to the triazole fungicides triadimefon, tebuconazole and difenoconazole, but not to the multisite fungicide chlorothalonil or to the MAP/histidine-kinase inhibitor fungicide fludioxonil. Based on the Y123H mutation in FvCYP51B, the four resistant mutants were subdivided into two genotypes: PCZ-R1 mutants with wildtype FvCYP51B and PCZ-R2 mutants with substitution Y123H in FvCYP51B. Wildtype FvCYP51B complemented the function of native ScCYP51 in Saccharomyces cerevisiae YUG37::erg11, whereas Y123H-mutated FvCYP51B did not. For the PCZ-R1 mutants, induced expression of FvCYP51A increased resistance to prochloraz. For the PCZ-R2 mutants, disruption of FvCYP51B function by the Y123H substitution caused constitutive up-regulation of FvCYP51A expression and thus resistance to prochloraz.

KeywordsAgronomy; Plant Sciences
Year of Publication2014
JournalPlant Pathology
Journal citation63 (4), pp. 952-960
Digital Object Identifier (DOI)https://doi.org/10.1111/ppa.12168
Open accessPublished as bronze (free) open access
FunderSpecial Fund for Agro-scientific Research in the Public Interest
National High Technology Research and Development Programme of China
Publisher's version
PublisherWiley
ISSN0032-0862

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