Mark Wilkinson

Name	Mark Wilkinson
Job title	Senior Scientific Specialist - Genetic Engineering
Email address	mark.wilkinson@rothamsted.ac.uk
Department	Plant Sciences and the Bioeconomy
Research cluster	PS: Resilience and Development
ORCID	https://orcid.org/0000-0002-4534-1728
Preferred citation	Wilkinson, M. D.
Office	Harpenden

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Research outputs

Genetic control of grain amino acid composition in a UK soft wheat mapping population

Oddy, J., Chhetry, M., Awal, R., Addy, J., Wilkinson, M. D., Smith, D., King, R., Hall, C., Testa, R., Murray, E., Raffan, S., Curtis, T. Y., Wingen, L., Griffiths, S., Berry, S., Elmore, S. J., Cryer, N., Moreira de Almeida, I. and Halford, N. G. 2023. Genetic control of grain amino acid composition in a UK soft wheat mapping population. The Plant Genome. 16 (4), p. e20335. https://doi.org/10.1002/tpg2.20335

Reducing dietary acrylamide exposure from wheat products through crop management and imaging

Oddy, J., Addy, J., Mead, A., Hall, C., Mackay, C., Ashfield, T., Mcdiarmid, F., Curtis, T. Y., Raffan, S., Wilkinson, M. D., Elmore, J. S., Cryer, N., Moreira de Almeida, I. and Halford, N. G. 2023. Reducing dietary acrylamide exposure from wheat products through crop management and imaging. Journal of Agricultural and Food Chemistry - JAFC. 71 (7), pp. 3403-3413. https://doi.org/10.1021/acs.jafc.2c07208

Novel molecules and target genes for vegetative heat tolerance in wheat

Rose, T., Wilkinson, M. D., Lowe, C., Xu, J., Hughes, D. J., Hassall, K. L., Hassani-Pak, K., Amberkar, S., Noleto-Dias, C., Ward, J. L. and Heuer, S. 2022. Novel molecules and target genes for vegetative heat tolerance in wheat. Plant Environmental Interactions. 3 (6), pp. 264-289. https://doi.org/10.1002/pei3.10096

Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1

Snell, P., Wilkinson, M. D., Taylor, G. J., Hall, S., Sharma, S., Sirijovski, N., Hansson, M., Shewry, P. R., Hofvander, P. and Grimberg, A. 2022. Characterisation of Grains and Flour Fractions from Field Grown Transgenic Oil-Accumulating Wheat Expressing Oat WRI1. Plants - Basel. 11 (7), p. 889. https://doi.org/10.3390/plants11070889

Understanding the Relationships between Free Asparagine in Grain and Other Traits to Breed Low-Asparagine Wheat

Oddy, J., Raffan, S., Wilkinson, M. D., Elmore, J. S. and Halford, N. G. 2022. Understanding the Relationships between Free Asparagine in Grain and Other Traits to Breed Low-Asparagine Wheat. Plants - Basel. 11 (5), p. 669. https://doi.org/10.3390/plants11050669

RNAi suppression of xylan synthase genes in wheat starchy endosperm

Wilkinson, M. D., Kosik, O., Halsey, K., Walpole, H., Evans, J., Wood, A. J., Ward, J. L., Mitchell, R. A. C., Lovegrove, A. and Shewry, P. R. 2021. RNAi suppression of xylan synthase genes in wheat starchy endosperm. PLOS ONE. 16 (8), p. e0256350. https://doi.org/10.1371/journal.pone.0256350

Reduced free asparagine in wheat grain resulting from a natural deletion of TaASNB2: investigating and exploiting diversity in the asparagine synthetase gene family to improve wheat quality

Oddy, J., Alarcon-Reverte, R., Wilkinson, M. D., Ravet, K., Raffan, S., Minter, A., Mead, A., Elmore, J. S., Moreira de Almeida, I., Cryer, N., Halford, N. G. and Pearce, S. 2021. Reduced free asparagine in wheat grain resulting from a natural deletion of TaASNB2: investigating and exploiting diversity in the asparagine synthetase gene family to improve wheat quality. BMC Plant Biology. 21 (1), p. 302. https://doi.org/10.1186/s12870-021-03058-7

The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis

Verhertbruggen, Y., Bouder, A., Vigouroux, J., Alvarado, C., Geairon, A., Guillon, F., Wilkinson, M. D., Stritt, F., Pauly, M., Lee, M. Y., Mortimer, J. C., Scheller, H. V., Mitchell, R. A. C., Voiniciuc, C., Saulnier, L. and Chateigner-Boutin, A.-L.. 2020. The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis . Plant Science. 302, p. 110693. https://doi.org/10.1016/j.plantsci.2020.110693

Stress, nutrients and genotype - understanding and managing asparagine accumulation in wheat grain.

Oddy, J., Raffan, S., Wilkinson, M. D., Elmore, J. S. and Halford, N. G. 2020. Stress, nutrients and genotype - understanding and managing asparagine accumulation in wheat grain. CABI Agriculture and Bioscience. 1, p. 10. https://doi.org/10.1186/s43170-020-00010-x

Effect of Plant Growth Promoting Bacteria on the Growth of Wheat Seedlings Subjected to Phosphate Starvation

Cataldi, M. P., Heuer, S., Mauchline, T. H., Wilkinson, M. D., Masters-Clark, E., Di Benedetto, N. A., Corbo, M. R. and Flagella, Z. 2020. Effect of Plant Growth Promoting Bacteria on the Growth of Wheat Seedlings Subjected to Phosphate Starvation . Agronomy. 10 (7), p. 978. https://doi.org/10.3390/agronomy10070978

Transitions in wheat endosperm metabolism upon transcriptional induction of oil accumulation by oat endosperm WRINKLED1

Grimberg, A., Wilkinson, M. D., Snell, P., De Vos, R. P., Gonzalez-Thuillier, I., Tawfike, A., Ward, J. L., Carlsson, A. S., Shewry, P. R. and Hofvander, P. 2020. Transitions in wheat endosperm metabolism upon transcriptional induction of oil accumulation by oat endosperm WRINKLED1 . BMC Plant Biology. 20, p. 235. https://doi.org/10.1186/s12870-020-02438-9

Sequence diversity and Identification of novel puroindoline and grain softness protein alleles in Elymus, Agropyron and related species

Wilkinson, M. D., King, R. and Grimaldi, R. 2018. Sequence diversity and Identification of novel puroindoline and grain softness protein alleles in Elymus, Agropyron and related species. Diversity. 10 (4), p. 114. https://doi.org/10.3390/d10040114

The Gsp-1 genes encode the wheat arabinogalactan peptide

Wilkinson, M. D., Tosi, P., Lovegrove, A., Corol, D. I., Ward, J. L., Palmer, R., Powers, S., Passmore, D., Webster, G., Marcus, S. E., Knox, J. P. and Shewry, P. R. 2017. The Gsp-1 genes encode the wheat arabinogalactan peptide. Quality and Nutrition at MonoGram 2017, 4-6 April 2017, University of Bristol, Bristol , UK (oral presentation). pp. p10

Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNAi lines with suppression of genes responsible for backbone synthesis and decoration

Freeman, J., Ward, J. L., Kosik, O., Lovegrove, A., Wilkinson, M. D., Shewry, P. R. and Mitchell, R. A. C. 2017. Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNAi lines with suppression of genes responsible for backbone synthesis and decoration. Plant Biotechnology Journal. 15 (11), pp. 1429-1438. https://doi.org/10.1111/pbi.12727

The Gsp-1 genes encode the wheat arabinogalactan peptide

Wilkinson, M. D., Tosi, P., Lovegrove, A., Corol, D. I., Ward, J. L., Palmer, R., Powers, S. J., Passmore, D., Webster, G., Marcus, S. E., Knox, J. P. and Shewry, P. R. 2017. The Gsp-1 genes encode the wheat arabinogalactan peptide. Journal of Cereal Science. 74 (March), pp. 155-164. https://doi.org/10.1016/j.jcs.2017.02.006

Effect of suppression of arabinoxylan synthetic genes in wheat endosperm on chain length of arabinoxylan and extract viscosity

Freeman, J., Lovegrove, A., Wilkinson, M. D., Saulnier, L., Shewry, P. R. and Mitchell, R. A. C. 2016. Effect of suppression of arabinoxylan synthetic genes in wheat endosperm on chain length of arabinoxylan and extract viscosity. Plant Biotechnology Journal. 14 (1), pp. 109-116. https://doi.org/10.1111/pbi.12361

Improving wheat as a source of dietary fibre for human health

Shewry, P. R., Reynolds, S., Pellny, T. K., Freeman, J., Wilkinson, M. D., Kosik, O., Ulker, M., Wingen, L., Orford, S., Griffiths, S., Mitchell, R. A. C. and Lovegrove, A. 2015. Improving wheat as a source of dietary fibre for human health. Cambridge University Press (CUP) Cambridge. https://doi.org/10.1017/S0029665115001020

RNA interference suppression of genes in glycosyl transferase families 43 and 47 in wheat starchy endosperm causes large decreases in arabinoxylan content

Lovegrove, A., Wilkinson, M. D., Freeman, J., Pellny, T. K., Tosi, P., Saulnier, L., Shewry, P. R. and Mitchell, R. A. C. 2013. RNA interference suppression of genes in glycosyl transferase families 43 and 47 in wheat starchy endosperm causes large decreases in arabinoxylan content. Plant Physiology. 163, pp. 95-107. https://doi.org/10.1104/pp.113.222653

Diversity of sequences encoded by the Gsp-1 genes in wheat and other grass species

Wilkinson, M. D., Castells-Brooke, N. I. D. and Shewry, P. R. 2013. Diversity of sequences encoded by the Gsp-1 genes in wheat and other grass species. Journal of Cereal Science. 57, pp. 1-9. https://doi.org/10.1016/j.jcs.2012.09.005

Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses

Anders, N., Wilkinson, M. D., Lovegrove, A., Freeman, J., Tryfona, T., Pellny, T. K., Weimar, T., Mortimer, J. C., Stott, K., Baker, J. M., Defoin-Platel, M., Shewry, P. R., Dupree, P. and Mitchell, R. A. C. 2012. Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses. Proceedings of the National Academy of Sciences. 109 (3), pp. 989-993. https://doi.org/10.1073/pnas.1115858109

Challenges and opportunities for using wheat for biofuel production

Shewry, P. R., Freeman, J., Wilkinson, M. D., Pellny, T. K. and Mitchell, R. A. C. 2011. Challenges and opportunities for using wheat for biofuel production. in: Halford, N. G. and Karp, A. (ed.) Energy Crops London Royal Society of Chemistry (RSC). pp. 13-26 Chapter 2

Challenges and opportunities for using wheat for biofuel production

Shewry, P. R., Freeman, J., Wilkinson, M. D., Pellny, T. K. and Mitchell, R. A. C. 2011. Challenges and opportunities for using wheat for biofuel production. in: Karp, A. and Halford, N. G. (ed.) Energy crops Royal Society of Chemistry (RSC), Cambridge. pp. 13-26

Temporal and spatial changes in cell wall composition in developing grains of wheat cv. Hereward

Toole, G. A., Le Gall, G., Colquhoun, I. J., Nemeth, C., Saulnier, L., Lovegrove, A., Pellny, T. K., Wilkinson, M. D., Freeman, J., Mitchell, R. A. C., Mills, E. N. C. and Shewry, P. R. 2010. Temporal and spatial changes in cell wall composition in developing grains of wheat cv. Hereward. Planta. 232, pp. 677-689. https://doi.org/10.1007/s00425-010-1199-5

Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat

Nemeth, C., Freeman, J., Jones, H. D., Sparks, C. A., Pellny, T. K., Wilkinson, M. D., Dunwell, J., Andersson, A. A. M., Aman, P., Guillon, F., Saulnier, L., Mitchell, R. A. C. and Shewry, P. R. 2010. Down-regulation of the CSLF6 gene results in decreased (1,3;1,4)-beta-D-glucan in endosperm of wheat. Plant Physiology. 152, pp. 1209-1218. https://doi.org/10.1104/pp.109.151712

Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality

Tamas, C., Kisgyorgy, B. N., Rakszegi, M., Wilkinson, M. D., Yang, M-S., Lang, L., Tamas, L. and Bedo, Z. 2009. Transgenic approach to improve wheat (Triticum aestivum L.) nutritional quality. Plant Cell Reports. 28, pp. 1085-1094. https://doi.org/10.1007/s00299-009-0716-0

Puroindoline genes and proteins in tetraploid and hexaploid species of Triticum

Rakszegi, M., Wilkinson, M. D., Tosi, P., Lovegrove, A., Kovacs, G., Bedo, Z. and Shewry, P. R. 2009. Puroindoline genes and proteins in tetraploid and hexaploid species of Triticum. Journal of Cereal Science. 49, pp. 202-211. https://doi.org/10.1016/j.jcs.2008.09.006

Identification and genetic mapping of variant forms of puroindoline b expressed in developing wheat grain

Wilkinson, M. D., Wan, Y., Tosi, P., Leverington, M., Snape, J., Mitchell, R. A. C. and Shewry, P. R. 2008. Identification and genetic mapping of variant forms of puroindoline b expressed in developing wheat grain. Journal of Cereal Science. 48, pp. 722-728. https://doi.org/10.1016/j.jcs.2008.03.007

Understanding the relationship between DNA methylation and phenotypic plasticity in crop plants

Amoah, S., Wilkinson, M. D., Dunwell, J. and King, G. J. 2008. Understanding the relationship between DNA methylation and phenotypic plasticity in crop plants. https://doi.org/10.1016/j.cbpa.2008.04.364

Decreased shoot stature and grain alpha-amylase activity following ectopic expression of a gibberellin 2-oxidase gene in transgenic wheat

Appleford, N. E., Wilkinson, M. D., Ma, Q., Evans, D. J., Stone, M. C., Pearce, S. P., Powers, S. J., Thomas, S. G., Jones, H. D., Phillips, A. L., Hedden, P. and Lenton, J. R. 2007. Decreased shoot stature and grain alpha-amylase activity following ectopic expression of a gibberellin 2-oxidase gene in transgenic wheat. Journal of Experimental Botany. 58, pp. 3213-3226. https://doi.org/10.1093/jxb/erm166

Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance

Yang, Y., Ma, Y. Z., Xu, Z. S., Chen, X. M., He, Z. H., Yu, Z., Wilkinson, M. D., Jones, H. D., Shewry, P. R. and Xia, L-Q. 2007. Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. Journal of Experimental Botany. 58, pp. 2863-2871. https://doi.org/10.1093/jxb/erm073

High throughput Agrobacterium transformation of wheat: a tool for functional genomics

Jones, H. D., Wilkinson, M. D., Doherty, A. and Wu, H. 2007. High throughput Agrobacterium transformation of wheat: a tool for functional genomics. Buck, H. T., Salomon, N. and Nisi, J. E. (ed.) Wheat Production in Stressed Environments: Proceedings 7th International Wheat Conference, Mar del Plata, Argentina, 27 November-2 December 2005 . Springer, Dordrecht. pp. 693-699

Molecular cloning of beta-kafirin, a methionine-rich protein of sorghum grain

Chamba, E. B., Halford, N. G., Forsyth, J., Wilkinson, M. D. and Shewry, P. R. 2005. Molecular cloning of beta-kafirin, a methionine-rich protein of sorghum grain. Journal of Cereal Science. 41 (3), pp. 381-383. https://doi.org/10.1016/j.jcs.2004.09.004

Transcripts of Vp-1 homoeologues are alternatively spliced within the Triticeae tribe

Wilkinson, M. D., Lenton, J. R. and Holdsworth, M. J. 2005. Transcripts of Vp-1 homoeologues are alternatively spliced within the Triticeae tribe. Euphytica. 143, pp. 243-246. https://doi.org/10.1007/s10681-005-7856-2

Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes

Chen, M., Wilkinson, M. D., Tosi, P., He, G. and Shewry, P. R. 2005. Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes. Theoretical and Applied Genetics. 111, pp. 1159-1166.

Production and characterization of transgenic wheat with altered gibberellin catabolism

Evans, D. J., Stone, M., Appleford, N. E. J., Wilkinson, M. D., Jones, H. D., Ma, Q., Croker, S. J., Phillips, A. L., Hedden, P. and Lenton, J. R. 2003. Production and characterization of transgenic wheat with altered gibberellin catabolism.

Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species

Wilkinson, M. D., Flintham, J., Gale, M., Lenton, J. R. and Holdsworth, M. J. 2003. Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species.

Transcripts of Vp-1 homologues are misspliced in modern wheat and ancestral species

Wilkinson, M. D., Lenton, J. R. and Holdsworth, M. J. 2003. Transcripts of Vp-1 homologues are misspliced in modern wheat and ancestral species. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003 . pp. 1069-1071

Use of comparative molecular genetics to study pre harvest sprouting in wheat

Wilkinson, M. D., Mckibbin, R. S., Bailey, P. C., Flintham, J. E., Gale, M. D., Lenton, J. R. and Holdsworth, M. J. 2002. Use of comparative molecular genetics to study pre harvest sprouting in wheat. Euphytica. 126, pp. 27-33.

Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species

Mckibbin, R. S., Wilkinson, M. D., Bailey, P. C., Flintham, J. E., Andrew, L. M., Lazzeri, P. A., Gale, M. D., Lenton, J. R. and Holdsworth, M. J. 2002. Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species. Proceedings of the National Academy of Sciences. 99 (15), pp. 10203-10208. https://doi.org/10.1073/pnas.152318599

Transformation of elite wheat varieties for improved agronomic and end-use qualities: modification of lipid biosynthesis and gibberellin levels

Wilkinson, M. D., Edlin, D. A. N., Kille, P., Harwood, J. L. and Jones, H. D. 2001. Transformation of elite wheat varieties for improved agronomic and end-use qualities: modification of lipid biosynthesis and gibberellin levels. Plants of the Balkan Peninsula: into the Next Millennium. Proceedings 2nd Balkan Botanical Congress, Istanbul, 14-18 May 2000 .

Age-dependent transformation frequency in elite wheat varieties

Pastori, G. M., Wilkinson, M. D., Steele, S. H., Sparks, C. A., Jones, H. D. and Parry, M. A. J. 2001. Age-dependent transformation frequency in elite wheat varieties. Journal of Experimental Botany. 52, pp. 857-863.

Hormonal and developmental regulation of alpha-amylase in transgenic wheat

Stone, M. C., Wilkinson, M. D., Huttly, A. K., Lenton, J. R. and Jones, H. D. 2001. Hormonal and developmental regulation of alpha-amylase in transgenic wheat. Abstracts SEB Annual Meeting, Canterbury, 2-6 April 2001 . pp. P7.78

Transformation of elite wheat varieties for improved end-use qualities: modification of gibberellin levels and pre-harvest sprouting

Jones, H. D. and Wilkinson, M. D. 2001. Transformation of elite wheat varieties for improved end-use qualities: modification of gibberellin levels and pre-harvest sprouting. Lang, L. and Bedo, Z. (ed.) Wheat in a Global Environment: Proceedings 6th International Wheat Conference, Budapest, 5-9 June 2000 . Kluwer Academic Publishers, Dordrecht, Netherlands. pp. 203-209

Morphological and metabolic changes in transgenic wheat with altered glycerol-3-phosphate acyltransferase or acyl-acyl carrier protein (ACP) thioesterase activities

Edlin, D. A. N., Kille, P., Wilkinson, M. D., Jones, H. D. and Harwood, J. L. 2000. Morphological and metabolic changes in transgenic wheat with altered glycerol-3-phosphate acyltransferase or acyl-acyl carrier protein (ACP) thioesterase activities. Biochemical Society Transactions. 28 (6), pp. 682-683. https://doi.org/10.1042/0300-5127:0280682

The effect of agriculturally relevant environmental factors on the expression and stability of genes affecting wheat lipids

Wilkinson, M. D., Edlin, D. A. N., Lazzeri, P. A., Kille, P., Harwood, J. L. and Jones, H. D. 2000. The effect of agriculturally relevant environmental factors on the expression and stability of genes affecting wheat lipids. Abstracts Annual Meeting of the Society for Experimental Biology, Exeter, 27-31 March 2000 . pp. C3.1

The effect of agriculturally-relevant environmental factors on the expression and stability of genes affecting wheat lipids

Wilkinson, M. D., Edlin, D. A. N., Harwood, J. L., Lazzeri, P. A. and Jones, H. D. 1999. The effect of agriculturally-relevant environmental factors on the expression and stability of genes affecting wheat lipids. Abstracts 16th Long Ashton International Symposium on Biotechnology of Cereals: Tools, Targets and Triumphs, Long Ashton 13-15 March 1999 .

Effects on leaf lipid metabolism in transgenic wheat varieties containing the Pisum sativum glycerol 3-phosphate acyltransferase gene

Edlin, D. A. N., Kille, P., Wilkinson, M. D., Jones, H. D. and Harwood, J. L. 1999. Effects on leaf lipid metabolism in transgenic wheat varieties containing the Pisum sativum glycerol 3-phosphate acyltransferase gene. Abstracts 16th Long Ashton International Symposium on Biotechnology of Cereals: Tools, Targets and Triumphs, Long Ashton, 13-15 September 1999 .

The effect of agriculturally-relevant environmental factors on the expression and stability of genes affecting wheat lipids

Wilkinson, M. D., Edlin, D. A. N., Harwood, J. L., Lazzeri, P. A. and Jones, H. D. 1999. The effect of agriculturally-relevant environmental factors on the expression and stability of genes affecting wheat lipids. Abstracts SET for Britain 99, House of Commons, London, 10 March 1999 .