A - Papers appearing in refereed journals
Hedden, P., Phillips, A. L., Rojas, M. C., Carrera, E. and Tudzynski, B. 2001. Gibberellin Biosynthesis in Plants and Fungi: A Case of Convergent Evolution? Journal of Plant Growth Regulation. 20 (4), pp. 319-331.
|Authors||Hedden, P., Phillips, A. L., Rojas, M. C., Carrera, E. and Tudzynski, B.|
As well as being phytohormones, gibberellins (GAs) are present in some fungi and bacteria. Indeed, GAs were first discovered in the fungus Gibberella fujikuroi, from which gibberellic acid (GA3) and other GAs are produced commercially. Although higher plants and the fungus produce structurally identical GAs, there are important differences in the pathways and enzymes involved. This has become particularly apparent with the identification of almost all of the genes for GA-biosynthesis in Arabidopsis thaliana and G. fujikuroi, following the sequencing of the Arabidopsis genome and the detection of a GA-biosynthesis gene cluster in the fungus. For example, 3b-hydroxylation occurs early in the pathway in G. fujikuroi and is catalyzed by a cytochrome P450 monooxygenase, whereas it is usually the final step in plants and is catalyzed by 2-oxoglutarate-dependent dioxygenases. Similarly, 20-oxidation is catalyzed by dioxygenases in plants and a cytochrome P450 in the fungus. Even where cytochrome P450s have equivalent functions in plants and Gibberella, they are unrelated in terms of amino acid sequence. These profound differences indicate that higher plants and fungi have evolved their complex biosynthetic pathways to GAs independently and not by horizontal gene transfer.
|Keywords||Arabidopsis thaliana; Gibberella fujikuroi; Gibberellin biosynthesis|
|Year of Publication||2001|
|Journal||Journal of Plant Growth Regulation|
|Journal citation||20 (4), pp. 319-331|
|Digital Object Identifier (DOI)||doi:10.1007/s003440010037|
|Open access||Published as non-open access|
|Funder||Biotechnology and Biological Sciences Research Council|
|Funder project or code||Regulation of gibberellin biosynthesis|
|Molecular cloning, function and structure of gibberellin-biosynthetic enzymes|
|Online||26 Mar 2002|
|Copyright license||Publisher copyright|
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