Structural and protein engineering studies of novel cereal and oilseed proteins with functional properties or biological activity

Project (past)

Project dates01 Apr 1999 to end of 31 Mar 2008
Project number4157
DepartmentCrop Performance and Improvement (CPI) Division
Plant Sciences (PSC)
Research fish codeBBS/E/C/00004157
Grant IDBBS/E/C/00004157

Outputs

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A novel bioinformatics approach identifies candidate genes for the synthesis and feruloylation of arabinoxylan

A - Papers appearing in refereed journals
Mitchell, R. A. C., Dupree, P. and Shewry, P. R. 2007. A novel bioinformatics approach identifies candidate genes for the synthesis and feruloylation of arabinoxylan. Plant Physiology. 144 (May), pp. 43-53. https://doi.org/10.1104/pp.106.094995

Amino acid sequence and molecular modelling of a lipid transfer protein from sunflower (Helianthus annus L.) seeds

A - Papers appearing in refereed journals
Luckett, S., Sessions, R. B., Michaelson, L. V., Naldrett, M. J., Clarke, A. R., Fido, R. J., Tatham, A. S. and Shewry, P. R. 2001. Amino acid sequence and molecular modelling of a lipid transfer protein from sunflower (Helianthus annus L.) seeds. Protein And Peptide Letters. 8, pp. 241-248.

An unusual helix-turn-helix protease inhibitory motif in a novel trypsin inhibitor from seeds of veronica (Veronica hederifolia L.)

A - Papers appearing in refereed journals
Conners, R., Konarev, A. V., Forsyth, J., Lovegrove, A., Marsh, J., Joseph-Horne, T., Shewry, P. R. and Brady, R. L. 2007. An unusual helix-turn-helix protease inhibitory motif in a novel trypsin inhibitor from seeds of veronica (Veronica hederifolia L.). Journal of Biological Chemistry. 282, pp. 27760-27768. https://doi.org/10.1074/jbc.M703871200

D hordeins of Hordeum chilense : a novel source of variation for improvement of wheat

A - Papers appearing in refereed journals
Piston, F., Shewry, P. R. and Barro, F. 2007. D hordeins of Hordeum chilense : a novel source of variation for improvement of wheat. Theoretical and Applied Genetics. 115, pp. 77-86. https://doi.org/10.1007/s00122-007-0542-0

Design, expression and characterisation of lysine-rich forms of the barley seed protein CI-2

A - Papers appearing in refereed journals
Forsyth, J. L., Beaudoin, F., Halford, N. G., Sessions, R. B., Clarke, A. R. and Shewry, P. R. 2005. Design, expression and characterisation of lysine-rich forms of the barley seed protein CI-2. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1747, pp. 221-227. https://doi.org/10.1016/j.bbapap.2004.11.018

Developing allergen-free foods by genetic manipulation

B - Book chapters etc edited externally
Shewry, P. R. and Jones, H. D. 2007. Developing allergen-free foods by genetic manipulation. in: Mills, C., Hoffmann-Sommergruber, K. and Wichers, H. (ed.) Managing allergens in food Woodhead Publishing, Cambridge. pp. 147-158

Essential amino acids

G - Articles in popular magazines and other technical publications
Shewry, P. R. 2006. Essential amino acids.

Expression of the gamma-zein protein of maize in seeds of transgenic barley: effects on grain composition and properties

A - Papers appearing in refereed journals
Zhang, Y., Darlington, H., Jones, H. D., Halford, N. G., Napier, J. A., Davey, M. R., Lazzeri, P. A. and Shewry, P. R. 2003. Expression of the gamma-zein protein of maize in seeds of transgenic barley: effects on grain composition and properties. Theoretical and Applied Genetics. 106 (6), pp. 1139-1146. https://doi.org/10.1007/s00122-002-1162-3

Food allergens of plant origin - their molecular and evolutionary relationships

A - Papers appearing in refereed journals
Mills, E. N. C., Madsen, C., Shewry, P. R. and Wichers, H. J. 2003. Food allergens of plant origin - their molecular and evolutionary relationships. Trends in Food Science and Technology. 14, pp. 145-156.

Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province

A - Papers appearing in refereed journals
Liu, Y., Xiong, Z-Y., He, Y-G., Shewry, P. R. and He, G-Y. 2007. Genetic diversity of HMW glutenin subunit in Chinese common wheat (Triticum aestivum L.) landraces from Hubei province. Genetic Resources and Crop Evolution. 54, pp. 865-874. https://doi.org/10.1007/s10722-006-9154-9

Harnessing sorghum and millet biotechnology for food and health

A - Papers appearing in refereed journals
O'kennedy, M. M., Grootboom, A. and Shewry, P. R. 2006. Harnessing sorghum and millet biotechnology for food and health. Journal of Cereal Science. 44, pp. 224-235. https://doi.org/10.1016/j.jcs.2006.08.001

Hazelnut allergy in outpatient clinics across twelve European countries (EuroPrevall study)

C2 - Non-edited contributions to conferences
Datema, M. R., Zuidmeer-Jongejan, L., Garino, C., Marsh, J., Lovegrove, A., Clausen, M., Gislason, D., Dubakiene, R., Reig, I., Barreales, L., Knulst, A., Le, T-M., Kowalski, M., Jedvzejczak-Czechowicz, M., Lidhlom, J., Bures, P., Kralimarkove, T., Popov, T., Asero, R., Seneviratne, S. L., Belohlavkova, S., Sinaniotis, N., Papadopoulos, N., Purovit, A., De Blay, F., Vieths, S., Fernandez-Rivas, M., Hoffman-Sommergruber, K., Van Ree, R. and Ballmer-Weber, B. 2013. Hazelnut allergy in outpatient clinics across twelve European countries (EuroPrevall study). World Allergy and Asthma Congress of the European Academy of Allergy and Clinical Immunology and World Allergy Organization, Milan, Italy, 22-26 June 2013 . Wiley. https://doi.org/10.1111/all.12248

Heterologous expression and protein engineering of wheat gluten proteins

A - Papers appearing in refereed journals
Tamas, L. and Shewry, P. R. 2006. Heterologous expression and protein engineering of wheat gluten proteins. Journal of Cereal Science. 43, pp. 259-274. https://doi.org/10.1016/j.jcs.2006.02.001

Identification and molecular characterisation of hordoindolines from barley grain

A - Papers appearing in refereed journals
Darlington, H. F., Rouster, J., Hoffmann, L., Halford, N. G., Shewry, P. R. and Simpson, D. J. 2001. Identification and molecular characterisation of hordoindolines from barley grain. Plant Molecular Biology. 47, pp. 785-794.

Identification of lipid transfer protein (LTP) as a major sunflower seed food allergen in sunflower seed-induced systemic reactions

C2 - Non-edited contributions to conferences
Papageorgiou, P. S., Shewry, P. R., Fido, R. J., Papadopoulos, N. G., Tassios, I. K. and Tatham, A. S. 2003. Identification of lipid transfer protein (LTP) as a major sunflower seed food allergen in sunflower seed-induced systemic reactions. Abstracts Greek National Meeting on Allergy and Clinical Immunology, Athens, April 2003 .

Identification of the wheat seed protein CM3 as a highly active emulsifier using a novel functional screen

A - Papers appearing in refereed journals
Gilbert, S. M., Burnett, G. R., Mills, E. N. C., Belton, P. S., Shewry, P. R. and Tatham, A. S. 2003. Identification of the wheat seed protein CM3 as a highly active emulsifier using a novel functional screen. Journal of Agricultural and Food Chemistry - JAFC. 51, pp. 2019-2025.

Improving the nutritional quality and functional properties of seed proteins by genetic engineering

B - Book chapters etc edited externally
Shewry, P. R. 2002. Improving the nutritional quality and functional properties of seed proteins by genetic engineering. in: Oksman-Caldentey, K-M. and Barz, W. H. (ed.) Plant biotechnology and transgenic plants Marcel Dekker Inc, New York. pp. 283-304

Improving the nutritional quality of cereals by conventional and novel approaches

B - Book chapters etc edited externally
Shewry, P. R. 2008. Improving the nutritional quality of cereals by conventional and novel approaches. in: Hamaker, B. R. (ed.) Technology of functional cereal products Woodhead Publishing, Cambridge. pp. 159-183

Improving the protein content and composition of cereal grain

A - Papers appearing in refereed journals
Shewry, P. R. 2007. Improving the protein content and composition of cereal grain. Journal of Cereal Science. 46, pp. 239-250. https://doi.org/10.1016/j.jcs.2007.06.006

In vitro binding of puroindolines to wheat starch granules

A - Papers appearing in refereed journals
Bloch, H. A., Darlington, H. F. and Shewry, P. R. 2001. In vitro binding of puroindolines to wheat starch granules. Cereal chemistry. 78, pp. 74-78.

In vitro fermentation by human fecal microflora of wheat arabinoxylans

A - Papers appearing in refereed journals
Hughes, S. A., Shewry, P. R., Li, L., Gibson, G. R., Sanz, M. L. and Rastall, R. A. 2007. In vitro fermentation by human fecal microflora of wheat arabinoxylans. Journal of Agricultural and Food Chemistry - JAFC. 55, pp. 4589-4595. https://doi.org/10.1021/jf070293g

Interaction between protein allergens and model gastric emulsions

A - Papers appearing in refereed journals
Burnett, G. R., Wickham, M., Fillery-Travis, A., Robertson, J. A., Belton, P. S., Gilbert, S. M., Tatham, A. S., Shewry, P. R. and Mills, E. N. C. 2002. Interaction between protein allergens and model gastric emulsions. Biochemical Society Transactions. 30 (6), pp. 916-918. https://doi.org/10.1042/bst0300916

Interfacial properties of sunflower storage proteins

C2 - Non-edited contributions to conferences
Berecz, B., Mackie, A. R., Shewry, P. R., Tamas, L. and Mills, C. E. 2005. Interfacial properties of sunflower storage proteins. Wiley.

Kafirin structure and functionality

A - Papers appearing in refereed journals
Belton, P. S., Delgadillo, I., Halford, N. G. and Shewry, P. R. 2006. Kafirin structure and functionality. Journal of Cereal Science. 44, pp. 272-286. https://doi.org/10.1016/j.jcs.2006.05.004

Molecular analysis of "stay green" mutants in durum wheat

C2 - Non-edited contributions to conferences
Rampino, P., Spano, G., Trono, D., Di Fonzo, N., Shewry, P. R., Napier, J. A. and Perrotta, C. 2003. Molecular analysis of "stay green" mutants in durum wheat. Proceedings 10th International Wheat Genetics Symposium, Paestum, Italy, 1-6 September 2003. Vol. 2 - Poster Presentations . pp. 905-907

Molecular analysis of a durum wheat 'stay green' mutant: expression pattern of photosynthesis-related genes

A - Papers appearing in refereed journals
Rampino, P., Spano, G., Pataleo, S., Mita, G., Napier, J. A., Di Fonzo, N., Shewry, P. R. and Perrotta, C. 2006. Molecular analysis of a durum wheat 'stay green' mutant: expression pattern of photosynthesis-related genes. Journal of Cereal Science. 43, pp. 160-168. https://doi.org/10.1016/j.jcs.2005.07.004

Molecular cloning of beta-kafirin, a methionine-rich protein of sorghum grain

A - Papers appearing in refereed journals
Chamba, E. B., Halford, N. G., Forsyth, J., Wilkinson, M. D. and Shewry, P. R. 2005. Molecular cloning of beta-kafirin, a methionine-rich protein of sorghum grain. Journal of Cereal Science. 41 (3), pp. 381-383. https://doi.org/10.1016/j.jcs.2004.09.004

Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes

A - Papers appearing in refereed journals
Chen, M., Wilkinson, M. D., Tosi, P., He, G. and Shewry, P. R. 2005. Novel puroindoline and grain softness protein alleles in Aegilops species with the C, D, S, M and U genomes. Theoretical and Applied Genetics. 111, pp. 1159-1166.

Osborne fractions

G - Articles in popular magazines and other technical publications
Shewry, P. R. 2006. Osborne fractions.

Physiological characterization of 'stay green' mutants in durum wheat

A - Papers appearing in refereed journals
Spano, G., Di Fonzo, N., Perrotta, C., Platani, C., Ronga, G., Lawlor, D. W., Napier, J. A. and Shewry, P. R. 2003. Physiological characterization of 'stay green' mutants in durum wheat. Journal of Experimental Botany. 54, pp. 1415-1420. https://doi.org/10.1093/jxb/erg150

Plant biotechnology: transgenic crops

B - Book chapters etc edited externally
Shewry, P. R., Jones, H. D. and Halford, N. G. 2008. Plant biotechnology: transgenic crops. in: Stahl, U., Donalies, U. E. B. and Nevoigt, E. (ed.) Food Biotechnology Springer International Publishing Ag.

Plant food allergens

E - Books edited/written by staff member, alone/with others
Mills, E. N. C. and Shewry, P. R. (ed.) 2004. Plant food allergens. Blackwell Scientific Publications, Oxford.

Plant protein families and their relationships to food allergy

A - Papers appearing in refereed journals
Shewry, P. R., Beaudoin, F., Jenkins, J., Griffiths-Jones, S. and Mills, E. N. C. 2002. Plant protein families and their relationships to food allergy. Biochemical Society Transactions. 30 (6), pp. 906-910. https://doi.org/10.1042/bst0300906

Plant protein families as a basis for predicting the allergenicity of food proteins

B - Book chapters etc edited externally
Shewry, P. R., Jenkins, J., Beaudoin, F. and Mills, E. N. C. 2006. Plant protein families as a basis for predicting the allergenicity of food proteins. in: Wichers, H. J., Bogers, R. J., Savelkoul, H. F. J. and Gilissen, L. J. W. J. (ed.) Allergy matters: new approaches to allergy prevention and management Springer, Dordrecht. pp. 41-46

Polymorphism and inheritance of methione-rich 2S albumins in sunflower

A - Papers appearing in refereed journals
Anisimova, I. N., Konarev, Al. V., Gavrilova, V. A., Rozhkova, V. T., Fido, R. F., Tatham, A. S. and Shewry, P. R. 2003. Polymorphism and inheritance of methione-rich 2S albumins in sunflower. Euphytica. 129, pp. 99-107.

Preface to sorghum and millets reviews

G - Articles in popular magazines and other technical publications
Taylor, J. and Shewry, P. R. 2006. Preface to sorghum and millets reviews. Journal of Cereal Science. 44 (3), p. 223. https://doi.org/10.1016/j.jcs.2006.10.001

Promoter analysis and immunolocalisation show that puroindoline genes are exclusively expressed in starchy endosperm cells of wheat grain

A - Papers appearing in refereed journals
Wiley, P. R., Tosi, P., Evrard, A., Lovegrove, A., Jones, H. D. and Shewry, P. R. 2007. Promoter analysis and immunolocalisation show that puroindoline genes are exclusively expressed in starchy endosperm cells of wheat grain. Plant Molecular Biology. 64, pp. 125-135. https://doi.org/10.1007/s11103-007-9139-x

Protein chemistry of dicotyledonous grains

G - Articles in popular magazines and other technical publications
Shewry, P. R. 2004. Protein chemistry of dicotyledonous grains.

Protein superfamilies

G - Articles in popular magazines and other technical publications
Shewry, P. R. 2006. Protein superfamilies.

Protein synthesis and deposition

G - Articles in popular magazines and other technical publications
Shewry, P. R. 2004. Protein synthesis and deposition.