Wheat gluten proteins: their characterisation and role in determining the functional properties and end use quality of wheat

Project (past)

Project dates01 Apr 1999 to end of 31 Mar 2008
Project number4226
DepartmentCrop Performance and Improvement (CPI) Division
Plant Sciences (PSC)
Research fish codeBBS/E/C/00004226
Grant IDBBS/E/C/00004226

Outputs

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A metabolomic study of substantial equivalence of field-grown genetically modified wheat

A - Papers appearing in refereed journals
Baker, J. M., Hawkins, N. D., Ward, J. L., Lovegrove, A., Napier, J. A., Shewry, P. R. and Beale, M. H. 2006. A metabolomic study of substantial equivalence of field-grown genetically modified wheat. Plant Biotechnology Journal. 4 (4), pp. 381-392. https://doi.org/10.1111/j.1467-7652.2006.00197.x

An optimal pooling strategy applied to high-throughput screening for rare marker-free transformants

A - Papers appearing in refereed journals
Zhang, J., Xiao, Q., Li, K., Chen, M., Chang, J., Luo, L., Li, Y., Liu, Y., Shewry, P. R. and He, G. 2006. An optimal pooling strategy applied to high-throughput screening for rare marker-free transformants. Biotechnology Letters. 28, pp. 1537-1544. https://doi.org/10.1007/s10529-006-9129-0

Analysis of high-molecular-weight glutenin subunit compositions from Hubei commercial hexaploid wheat (Triticum aestivum L.) cultivars

A - Papers appearing in refereed journals
Liu, Y., He, G-Y., Li, T-E., Chen, M-J., He, Y-G. and Shewry, P. R. 2004. Analysis of high-molecular-weight glutenin subunit compositions from Hubei commercial hexaploid wheat (Triticum aestivum L.) cultivars. Journal of Wuhan Botanical Research. 22, pp. 105-110.

Are GM and conventionally bred cereals really different?

A - Papers appearing in refereed journals
Shewry, P. R., Baudo, M., Lovegrove, A., Powers, S. J., Napier, J. A., Ward, J. L., Baker, J. M. and Beale, M. H. 2007. Are GM and conventionally bred cereals really different? Trends in Food Science and Technology. 18, pp. 201-209. https://doi.org/10.1016/j.tifs.2006.12.010

Changes in protein secondary structure during gluten deformation studied by dynamic Fourier transform infrared spectroscopy

A - Papers appearing in refereed journals
Wellner, N., Mills, E. N. C., Brownsey, G., Wilson, R. H., Brown, N. A., Freeman, J., Halford, N. G., Shewry, P. R. and Belton, P. S. 2005. Changes in protein secondary structure during gluten deformation studied by dynamic Fourier transform infrared spectroscopy. Biomacromolecules. 6 (1), pp. 255-261. https://doi.org/10.1021/bm049584d

Chemical imaging: the distribution of ions and molecules in developing and mature wheat grain

A - Papers appearing in refereed journals
Mills, E. N. C., Parker, M. L., Wellner, N., Toole, G., Feeney, K. and Shewry, P. R. 2005. Chemical imaging: the distribution of ions and molecules in developing and mature wheat grain. Journal of Cereal Science. 41, pp. 193-201.

Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes

A - Papers appearing in refereed journals
Shewry, P. R., Powers, S. J., Field, J. M., Fido, R. J., Jones, H. D., Arnold, G. M., West, J., Lazzeri, P. A., Barcelo, P., Barro, F., Tatham, A. S., Bekes, F., Butow, B. and Darlington, H. 2006. Comparative field performance over 3 years and two sites of transgenic wheat lines expressing HMW subunit transgenes. Theoretical and Applied Genetics. 113, pp. 128-136. https://doi.org/10.1007/s00122-006-0279-1

Comparative structures and properties of elastic proteins

B - Book chapters etc edited externally
Tatham, A. S. and Shewry, P. R. 2003. Comparative structures and properties of elastic proteins. in: Shewry, P. R., Bailey, A. J. and Tatham, A. S. (ed.) Elastomeric proteins: structures, biomechanical properties and biological roles Cambridge University Press (CUP) Cambridge. pp. 338-351

Creating a balance - the incorporation of a HMW glutenin subunit into transgenic wheat lines

A - Papers appearing in refereed journals
Butow, B. J., Tatham, A. S., Savage, A. W. J., Gilbert, S. M., Shewry, P. R., Solomon, R. G. and Bekes, F. 2003. Creating a balance - the incorporation of a HMW glutenin subunit into transgenic wheat lines. Journal of Cereal Science. 38, pp. 181-187.

Developing allergen-free foods by genetic manipulation

B - Book chapters etc edited externally
Shewry, P. R. and Jones, H. D. 2007. Developing allergen-free foods by genetic manipulation. in: Mills, C., Hoffmann-Sommergruber, K. and Wichers, H. (ed.) Managing allergens in food Woodhead Publishing, Cambridge. pp. 147-158

Effects of restricted water availability and increased temperature on the grain filling, drying and quality of winter wheat

A - Papers appearing in refereed journals
Gooding, M. J., Ellis, R. H., Shewry, P. R. and Schofield, J. D. 2003. Effects of restricted water availability and increased temperature on the grain filling, drying and quality of winter wheat. Journal of Cereal Science. 37, pp. 295-309.

Elastomeric proteins: structures, biomechanical properties and biological roles

E - Books edited/written by staff member, alone/with others
Shewry, P. R. and Tatham, A. S. Bailey, A. J. (ed.) 2003. Elastomeric proteins: structures, biomechanical properties and biological roles. Cambridge University Press (CUP) Cambridge.

Electrophoresis of wheat gluten proteins

B - Book chapters etc edited externally
Khan, K., Nygard, G., Pogna, N. E., Redaelli, R., Ng, P. K. W. and Fido, R. J. 2003. Electrophoresis of wheat gluten proteins. in: Shewry, P. R. and Lookhart, G. L. (ed.) Wheat gluten protein analysis American Association of Cereal Chemists (AACC), St Paul, MN. pp. 31-59

Essential amino acids

G - Articles in popular magazines and other technical publications
Shewry, P. R. 2006. Essential amino acids.

Expression of an extended HMW subunit in transgenic wheat and the effect on dough mixing properties

A - Papers appearing in refereed journals
He, G. Y., Jones, H. D., D'ovidio, R., Masci, S., Chen, M., West, J., Butow, B., Anderson, O. D., Lazzeri, P. A., Fido, R. and Shewry, P. R. 2005. Expression of an extended HMW subunit in transgenic wheat and the effect on dough mixing properties. Journal of Cereal Science. 42, pp. 225-231.

Expression of epitope-tagged LMW glutenin subunits in the starchy endosperm of transgenic wheat and their incorporation into glutenin polymers

A - Papers appearing in refereed journals
Tosi, P., D'ovidio, R., Napier, J. A., Bekes, F. and Shewry, P. R. 2004. Expression of epitope-tagged LMW glutenin subunits in the starchy endosperm of transgenic wheat and their incorporation into glutenin polymers. Theoretical and Applied Genetics. 108, pp. 468-476.

From Research Station to Institute, 1975-2003

B - Book chapters etc edited externally
Brent, K. J. 2003. From Research Station to Institute, 1975-2003. in: Shewry, P. R., Lenton, J. R. and Anderson, H. M. (ed.) Long Ashton Research Station: one hundred years of science in support of agriculture Institute for Arable Crops Research (IACR) , Long Ashton Research Station (LARS), Bristol. pp. 65-76

Functional properties and agronomic performance of transgenic tritordeum expressing high molecular weight glutenin subunit genes 1Ax1 and 1Dx5

A - Papers appearing in refereed journals
Barro, F., Barcelo, P., Lazzeri, P. A., Shewry, P. R., Martin, A. and Ballesteros, J. 2003. Functional properties and agronomic performance of transgenic tritordeum expressing high molecular weight glutenin subunit genes 1Ax1 and 1Dx5. Journal of Cereal Science. 37, pp. 65-70.

Functional properties of flours from field grown transgenic wheat lines expressing the HMW glutenin subunit 1Ax1 and 1Dx5 genes

A - Papers appearing in refereed journals
Barro, F., Barcelo, P., Lazzeri, P. A., Shewry, P. R., Ballesteros, J. and Martin, A. 2003. Functional properties of flours from field grown transgenic wheat lines expressing the HMW glutenin subunit 1Ax1 and 1Dx5 genes. Molecular Breeding. 12, pp. 223-229.

Functional properties of wheat gliadins. I. Effects on mixing characteristics and bread making quality

A - Papers appearing in refereed journals
Khatkar, B. S., Fido, R. J., Tatham, A. S. and Schofield, J. D. 2002. Functional properties of wheat gliadins. I. Effects on mixing characteristics and bread making quality. Journal of Cereal Science. 35 (3), pp. 299-306. https://doi.org/10.1006/jcrs.2001.0429

Functional properties of wheat gliadins. II. Effects on dynamic rheological properties of wheat gluten

A - Papers appearing in refereed journals
Khatkar, B. S., Fido, R. J., Tatham, A. S. and Schofield, J. D. 2002. Functional properties of wheat gliadins. II. Effects on dynamic rheological properties of wheat gluten. Journal of Cereal Science. 35 (3), pp. 307-313. https://doi.org/10.1006/jcrs.2001.0430

Genetic manipulation of durum wheat: application to grain composition and quality

B - Book chapters etc edited externally
Shewry, P. R., Bell, P., Di Fonzo, N., Lamacchia, C., Tosi, P., Lazzeri, P. A. and Barcelo, P. 2005. Genetic manipulation of durum wheat: application to grain composition and quality. in: Araus, J. L., Slafer, G. A., Pfeiffer, W. H., Nachit, M. M., DiFonzo, N. and Royo, C. (ed.) Durum wheat breeding: current approaches and future strategies. Volume 1 Food Products Press, New York. pp. 225-253

Genetic manipulation of gluten structure and function

B - Book chapters etc edited externally
Shewry, P. R., Lafiandra, D. and Tamas, L. 2006. Genetic manipulation of gluten structure and function. in: Bekes, F., Bushuk, W. and Wrigley, C. (ed.) Gliadin and glutenin: the unique balance of wheat quality American Association of Cereal Chemists (AACC), St Paul, MN. pp. 363-385

Genetics of wheat gluten proteins

A - Papers appearing in refereed journals
Shewry, P. R., Halford, N. G. and Lafiandra, D. 2003. Genetics of wheat gluten proteins. Advances in Genetics. 49, pp. 111-184.

Gluten, the elastomeric protein of wheat seeds

B - Book chapters etc edited externally
Halford, N. G. and Belton, P. S. 2003. Gluten, the elastomeric protein of wheat seeds. in: Shewry, P. R., Bailey, A. J. and Tatham, A. S. (ed.) Elastomeric proteins: structures, biomechanical properties and biological roles Cambridge University Press (CUP) Cambridge. pp. 279-301

Heterologous expression and protein engineering of wheat gluten proteins

A - Papers appearing in refereed journals
Tamas, L. and Shewry, P. R. 2006. Heterologous expression and protein engineering of wheat gluten proteins. Journal of Cereal Science. 43, pp. 259-274. https://doi.org/10.1016/j.jcs.2006.02.001

Improving the protein content and composition of cereal grain

A - Papers appearing in refereed journals
Shewry, P. R. 2007. Improving the protein content and composition of cereal grain. Journal of Cereal Science. 46, pp. 239-250. https://doi.org/10.1016/j.jcs.2007.06.006

Improving wheat quality: the role of biotechnology

B - Book chapters etc edited externally
Shewry, P. R. 2003. Improving wheat quality: the role of biotechnology. in: Cauvain, S. P. (ed.) Bread making: improving quality Woodhead Publishing, Cambridge. pp. 168-186

Long Ashton Research Station: one hundred years of science in support of agriculture

E - Books edited/written by staff member, alone/with others
Anderson, H. M., Lenton, J. R. and Shewry, P. R. (ed.) 2003. Long Ashton Research Station: one hundred years of science in support of agriculture. Institute for Arable Crops Research (IACR) , Long Ashton Research Station (LARS), Bristol.

Manipulation of the composition and functional properties of wheat gluten by chromosome and genetic engineering

C1 - Edited contributions to conferences/learned societies
Shewry, P. R. and Lafiandra, D. 2003. Manipulation of the composition and functional properties of wheat gluten by chromosome and genetic engineering. Stanca, A. M., Mare, C. and Faccoili, P. (ed.) Proceedings Cereal Section Meeting of the European Association for Research on Plant Breeding: From Biodiversity to Genomics: Breeding Strategies for Small Grain Cereals in the Third Millennium, Salsomaggiore, 21-25 November 2002 . Experimental Institute for Cereal Research, Fiorenzuola d'Arda. pp. 313-317

Milling and baking properties of field grown wheat expressing HMW subunit transgenes

A - Papers appearing in refereed journals
Darlington, H., Fido, R. F., Tatham, A. S., Jones, H. D., Salmon, S. E. and Shewry, P. R. 2003. Milling and baking properties of field grown wheat expressing HMW subunit transgenes. Journal of Cereal Science. 38, pp. 301-306.

Modification of the low molecular weight (LMW) glutenin composition of transgenic durum wheat: effects on glutenin polymer size and gluten functionality

A - Papers appearing in refereed journals
Tosi, P., Masci, S., Giovangrossi, A., D'ovidio, R., Bekes, F., Larroque, O., Napier, J. A. and Shewry, P. R. 2005. Modification of the low molecular weight (LMW) glutenin composition of transgenic durum wheat: effects on glutenin polymer size and gluten functionality. Molecular Breeding. 16, pp. 113-126.

Molecular structures and interactions of repetitive peptides based on wheat glutenin subunits depend on chain length

A - Papers appearing in refereed journals
Feeney, K. A., Wellner, N., Gilbert, S. M., Halford, N. G., Tatham, A. S., Shewry, P. R. and Belton, P. S. 2003. Molecular structures and interactions of repetitive peptides based on wheat glutenin subunits depend on chain length. Biopolymers (Biospectroscopy). 72, pp. 123-131.

Nanomechanical force measurements of gliadin protein interactions

A - Papers appearing in refereed journals
Paananen, A., Tappura, K., Tatham, A. S., Fido, R., Shewry, P. R., Miles, M. and Mcmaster, T. J. 2006. Nanomechanical force measurements of gliadin protein interactions. Biopolymers. 83 (6), pp. 658-667. https://doi.org/10.1002/bip.20603

Osborne fractions

G - Articles in popular magazines and other technical publications
Shewry, P. R. 2006. Osborne fractions.

Plant biotechnology: transgenic crops

B - Book chapters etc edited externally
Shewry, P. R., Jones, H. D. and Halford, N. G. 2008. Plant biotechnology: transgenic crops. in: Stahl, U., Donalies, U. E. B. and Nevoigt, E. (ed.) Food Biotechnology Springer International Publishing Ag.

Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength

C2 - Non-edited contributions to conferences
Maruyama-Funatsuki, W., Tosi, P., Jones, H. D., Sparks, C. A., Riley, A., Yamauchi, H. and Shewry, P. R. 2007. Production of transgenic wheat transformed with low-molecular-weight glutenin genes aiming for the understanding of the dough strength.

Protein chemistry of dicotyledonous grains

G - Articles in popular magazines and other technical publications
Shewry, P. R. 2004. Protein chemistry of dicotyledonous grains.

Protein extraction from plant tissues

B - Book chapters etc edited externally
Fido, R. J., Mills, E. N. C., Rigby, N. M. and Shewry, P. R. 2004. Protein extraction from plant tissues. in: Cutler, P. (ed.) Protein purification protocols, 2nd ed. Humana Press, Totowa. pp. 21-27

Protein superfamilies

G - Articles in popular magazines and other technical publications
Shewry, P. R. 2006. Protein superfamilies.