A - Papers appearing in refereed journals
Arnold, T., Kirk, G. J. D., Wissuwa, M., Frei, M., Zhao, F-J., Mason, T. F. D. and Weiss, D. J. 2010. Evidence for the mechanisms of zinc uptake by rice using isotope fractionation. Plant, Cell and Environment. 33 (3), pp. 370-381.
|Authors||Arnold, T., Kirk, G. J. D., Wissuwa, M., Frei, M., Zhao, F-J., Mason, T. F. D. and Weiss, D. J.|
In an earlier study, we found that rice (Oryza sativa) grown in nutrient solution well-supplied with Zn preferentially took up light 64Zn over 66Zn, probably as a result of kinetic fractionation in membrane transport processes. Here, we measure isotope fractionation by rice in a submerged Zn-deficient soil with and without Zn fertilizer. We grew the same genotype as in the nutrient solution study plus low-Zn tolerant and intolerant lines from a recombinant inbred population. In contrast to the nutrient solution, in soil with Zn fertilizer we found little or heavy isotopic enrichment in the plants relative to plant-available Zn in the soil, and in soil without Zn fertilizer we found consistently heavy enrichment, particularly in the low-Zn tolerant line. These observations are only explicable by complexation of Zn by a complexing agent released from the roots and uptake of the complexed Zn by specific root transporters. We show with a mathematical model that, for realistic rates of secretion of the phytosiderophore deoxymugineic acid (DMA) by rice, and realistic parameters for the Zn-solubilizing effect of DMA in soil, solubilization and uptake by this mechanism is necessary and sufficient to account for the measured Zn uptake and the differences between genotypes.
|Year of Publication||2010|
|Journal||Plant, Cell and Environment|
|Journal citation||33 (3), pp. 370-381|
|Digital Object Identifier (DOI)||doi:10.1111/j.1365-3040.2009.02085.x|
|Open access||Published as non-open access|
|Funder||Biotechnology and Biological Sciences Research Council|
|Funder project or code||SEF|
Permalink - https://repository.rothamsted.ac.uk/item/8q4z1/evidence-for-the-mechanisms-of-zinc-uptake-by-rice-using-isotope-fractionation