Rapid reduction of arsenate in the medium mediated by plant roots

A - Papers appearing in refereed journals

Xu, X-Y., McGrath, S. P. and Zhao, F-J. 2007. Rapid reduction of arsenate in the medium mediated by plant roots. New Phytologist. 176 (3), pp. 590-599.

AuthorsXu, X-Y., McGrath, S. P. and Zhao, F-J.
Abstract

Microbes detoxify arsenate by reduction and efflux of arsenite. Plants have a high capacity to reduce arsenate, but arsenic efflux has not been reported. Tomato (Lycopersicon esculentum) and rice (Oryza sativa) were grown hydroponically and supplied with 10 mu M marsenate or arsenite, with or without phosphate, for 1-3 d. The chemical species of As in nutrient solutions, roots and xylem sap were monitored, roles of microbes and root exudates in As transformation were investigated and efflux of As species from tomato roots was determined. Arsenite remained stable in the nutrient solution, whereas arsenate was rapidly reduced to arsenite. Microbes and root exudates contributed little to the reduction of external arsenate. Arsenite was the predominant species in roots and xylem sap. Phosphate inhibited arsenate uptake and the appearance of arsenite in the nutrient solution, but the reduction was near complete in 24 h in both -P- and +P-treated tomato. Phosphate had a greater effect in rice than tomato. Efflux of both arsenite and arsenate was observed; the former was inhibited and the latter enhanced by the metabolic inhibitor carbonylcyanide m-chlorophenylhydrazone. Tomato and rice roots rapidly reduce arsenate to arsenite, some of which is actively effluxed to the medium. The study reveals a new aspect of As metabolism in plants.

KeywordsPlant Sciences
Year of Publication2007
JournalNew Phytologist
Journal citation176 (3), pp. 590-599
Digital Object Identifier (DOI)doi:10.1111/j.1469-8137.2007.02195.x
PubMed ID17692074
Open accessPublished as non-open access
Funder project or codeSEF
ISSN0028646X
PublisherWiley

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