Physiological evidence for a high-affinity cadmium transporter highly expressed in a Thlaspi caerulescens ecotype

A - Papers appearing in refereed journals

Lombi, E., Zhao, F-J., McGrath, S. P., Young, S. D. and Sacchi, G. A. 2001. Physiological evidence for a high-affinity cadmium transporter highly expressed in a Thlaspi caerulescens ecotype. New Phytologist. 149 (1), pp. 53-60. https://doi.org/10.1046/j.1469-8137.2001.00003.x

AuthorsLombi, E., Zhao, F-J., McGrath, S. P., Young, S. D. and Sacchi, G. A.
Abstract

Uptake kinetics and translocation characteristics of cadmium and zinc are presented for two contrasting ecotypes of the Cd/Zn hyperaccumulator Thlaspi caerulescens, Ganges (southern France) and Prayon (Belgium). Experiments using radioactive isotopes were designed to investigate the physiology of Cd and Zn uptake, and a pressure-chamber system was employed to collect xylem sap. In contrast to similar Zn uptake and translocation, measurements of concentration-dependent influx of Cd revealed marked differences between ecotypes. Ganges alone showed a clear saturable component in the low Cd concentration range; maximum influx V(max) for Cd was fivefold higher in Ganges; and there was a fivefold difference in the Cd concentration in xylem sap. Addition of Zn to the uptake solution at equimolar concentration to Cd did not decrease Cd uptake by Ganges, but caused a 35% decrease in Prayon. There is strong physiological evidence for a high-affinity, highly expressed Cd transporter in the root cell plasma membranes of the Ganges ecotype of T. caerulescens. This raises evolutionary questions about specific transporters for non-essential metals. The results also show the considerable scope for selecting hyperaccumulator ecotypes to achieve higher phytoextraction efficiencies.

KeywordsPlant Sciences
Year of Publication2001
JournalNew Phytologist
Journal citation149 (1), pp. 53-60
Digital Object Identifier (DOI)https://doi.org/10.1046/j.1469-8137.2001.00003.x
Open accessPublished as non-open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or code443
512
Heavy metal uptake by plants
Remidiation of metal contaminated soils by plants (PHYTOREM)
Soil protection and remediation by chemical and biological approaches
Project: 4323
Output statusPublished
PublisherWiley
ISSN0028-646X

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