The trichothecene mycotoxin deoxynivalenol facilitates cell-to-cell invasion during wheat-tissue colonisation by Fusarium graminearum

A - Papers appearing in refereed journals

Armer, V., Urban, M., Ashfield, T., Deeks, M. J. and Hammond-Kosack, K. E. 2024. The trichothecene mycotoxin deoxynivalenol facilitates cell-to-cell invasion during wheat-tissue colonisation by Fusarium graminearum. Molecular Plant Pathology. 25 (6), p. e13485. https://doi.org/10.1111/mpp.13485

AuthorsArmer, V., Urban, M., Ashfield, T., Deeks, M. J. and Hammond-Kosack, K. E.
Abstract

Fusarium Head Blight (FHB) disease on small grain cereals is primarily caused by the ascomycete fungal pathogen Fusarium graminearum. Infection of floral spike tissues is characterised by the biosynthesis and secretion of potent trichothecene mycotoxins, of which deoxynivalenol (DON) is widely reported due to its negative impacts on grain quality and consumer safety. The TRI5 gene encodes an essential enzyme in the DON biosynthesis pathway and the single gene deletion mutant, ΔTri5, is widely reported to restrict disease progression to the inoculated spikelet. In this study, we present novel bioimaging evidence revealing that DON facilitates the traversal of the cell wall through plasmodesmata, a process essential for successful colonisation of host tissue. Chemical complementation of ΔTri5 did not restore macro- or microscopic phenotypes, indicating that DON secretion is tightly regulated both spatially and temporally. A comparative qualitative and quantitative morphological cellular analysis revealed infections had no impact on plant cell wall thickness. Immuno-labelling of callose at plasmodesmata during infection indicates that DON can increase deposits when applied exogenously, but is reduced when F. graminearum hyphae are present. This study highlights the complexity of the inter-connected roles of mycotoxin production, cell wall architecture and plasmodesmata in this highly specialised interaction.

KeywordsFusarium graminearum ; Deoxynivalenol (DON); Plasmodesmata; Wheat; Triticum aestivum; Head scab disease
Year of Publication2024
JournalMolecular Plant Pathology
Journal citation25 (6), p. e13485
Digital Object Identifier (DOI)https://doi.org/10.1111/mpp.13485
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeBBSRC SWBio DTP
Delivering Sustainable Wheat (WP2): Delivering Resilience to Biotic Stress
Designing Future Wheat (DFW) [ISPG]
UKRI/BBSRC-NSF/BIO Determining the Roles of Fusarium Effector Proteases in Plant Pathogenesis
Defining the signalling network linking pathogen infection and asparagine accumulation in wheat grain
Semiochemical-based alternative concepts for the management of wireworms
Publisher's version
Supplemental file
Output statusPublished
Publication dates
Online18 Apr 2024
Publication process dates
Accepted22 May 2024
PublisherPublic Library of Science (PLOS)
Cold Spring Harbor Lab Press, Publications Dept
Wiley
ISSN1464-6722

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