Methods for the purification of tomato bushy stunt and tobacco mosaic viruses

A - Papers appearing in refereed journals

Bawden, F. C. and Pirie, N. W. 1943. Methods for the purification of tomato bushy stunt and tobacco mosaic viruses. Biochemical Journal. 37 (1), pp. 66-70. https://doi.org/10.1042/bj0370066

AuthorsBawden, F. C. and Pirie, N. W.
Abstract

Since we first described methods for making liquid crystalline preparations of tobacco mosaic virus [Bawden & Pirie, 1937] and crystalline preparations of tomato bushy stunt virus [Bawden & Pirie, 1938], we have made preparations of both viruses by many other methods. As a result, we can now describe simpler and better procedures. Other workers have used differential ultracentrifugation; because of the infrequency with which stable substances of high molecular weight seem to occur in normal leaves, this method of preparation is undoubtedly of great value. The handling of large volumes of sap in the ultracentrifuge, however, is laborious, and many laboratories where plant viruses are studied do not have this equipment. Methods of preparation that are trustworthy and involve only ordinary centrifuges may therefore be of more general use.

Year of Publication1943
JournalBiochemical Journal
Journal citation37 (1), pp. 66-70
Digital Object Identifier (DOI)https://doi.org/10.1042/bj0370066
PubMed ID16747602
PubMed Central IDPMC1257845
Web address (URL)https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1257845/
Open accessPublished as bronze (free) open access
Publisher's version
Output statusPublished
Publication dates
Print01 Apr 1943
Copyright licensePublisher copyright
PublisherPortland Press Ltd
ISSN0264-6021

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