Factors affecting the amount of tobacco mosaic virus nucleic acid in phenol treated extracts from tobacco leaves

A - Papers appearing in refereed journals

Bawden, F. C. and Pirie, N. W. 1972. Factors affecting the amount of tobacco mosaic virus nucleic acid in phenol treated extracts from tobacco leaves. Proceedings of the Royal Society B: Biological Sciences. 182 (1068), pp. 297-318. https://doi.org/10.1098/rspb.1972.0081

AuthorsBawden, F. C. and Pirie, N. W.
Abstract

Extracts from the lower leaves of tobacco plants infected with tobacco mosaic virus and pulped in the presence of phenol are less infective than extracts from leaves pulped and then treated with phenol. Extracts from uninfected leaves mixed with purified virus behave similarly. The difference becomes progressively smaller as leaves are taken from further up the plant. The difference is smaller when the interval between pulping and adding phenol is short, and it is still smaller if air is rigidly excluded during the interval. Fixation of virus nucleic acid to the leaf fibre is the main factor responsible for this difference. Part of the normal nucleic acid of the leaf is fixed similarly. Fixation is partly prevented by including yeast nucleic acid in the extraction fluid, or by excluding Ca2+ from it by adding citrate. Part of the nucleic acid is held tenaciously by the fibre. The possible significance of this fixation in vivo in controlling the apparent susceptibility to infection of plants in different physiological states is discussed.

Year of Publication1972
JournalProceedings of the Royal Society B: Biological Sciences
Journal citation182 (1068), pp. 297-318
Digital Object Identifier (DOI)https://doi.org/10.1098/rspb.1972.0081
Open accessPublished as non-open access
Output statusPublished
Publication dates
Print01 Oct 1972
Online01 Jan 1997
Copyright licensePublisher copyright
ISSN0962-8452
PublisherRoyal Society Publishing

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