Transcriptomic and Metabolomic Analyses Reveal the Importance of Lipid Metabolism and Photosynthesis Regulation in High Salinity Tolerance in Barley (Hordeum vulgare L.) Leaves Derived from Mutagenesis Combined with Microspore Culture

A - Papers appearing in refereed journals

Xu, H., Halford, N. G., Guo, G., Chen, Z., Li, Y., Zhou, L, Liu, C. and Xu, R. 2023. Transcriptomic and Metabolomic Analyses Reveal the Importance of Lipid Metabolism and Photosynthesis Regulation in High Salinity Tolerance in Barley (Hordeum vulgare L.) Leaves Derived from Mutagenesis Combined with Microspore Culture. International Journal Of Molecular Sciences. 24 (23), p. 16757. https://doi.org/10.3390/ijms242316757

AuthorsXu, H., Halford, N. G., Guo, G., Chen, Z., Li, Y., Zhou, L, Liu, C. and Xu, R.
Abstract

Barley is the most salt-tolerant cereal crop. However, little attention has been paid to the salt-tolerant doubled haploids of barley derived from mutagenesis combined with isolated microspore culture. In the present study, barley doubled haploid (DH) line 20, which was produced by mutagenesis combined with isolated microspore culture, showed stably and heritably better salt tolerance than the wild type H30 in terms of fresh shoot weight, dry shoot weight, K+/Na+ ratio and photosynthetic characteristics. Transcriptome and metabolome analyses were performed to compare the changes in gene expression and metabolites between DH20 and H30. A total of 462 differentially expressed genes (DEGs) and 152 differentially accumulated metabolites (DAMs) were identified in DH20 compared to H30 under salt stress. Among the DAMs, fatty acids were the most accumulated in DH20 under salt stress. The integration of transcriptome and metabolome analyses revealed that nine key biomarkers, including two metabolites and seven genes, could distinguish DH20 and H30 when exposed to high salt. The pathways of linoleic acid metabolism, alpha-linolenic acid metabolism, glycerolipid metabolism, photosynthesis, and alanine, aspartate and glutamate metabolism were significantly enriched in DH20 with DEGs and DAMs in response to salt stress. These results suggest that DH20 may enhance resilience by promoting lipid metabolism, maintaining energy metabolism and decreasing amino acids metabolism. The study provided novel insights for the rapid generation of homozygous mutant plants by mutagenesis combined with microspore culture technology and also identified candidate genes and metabolites that may enable the mutant plants to cope with salt stress.

KeywordsBarley; Mutagenesis; Salt tolerance; Microspore; Transcriptome; Metabolome
Year of Publication2023
JournalInternational Journal Of Molecular Sciences
Journal citation24 (23), p. 16757
Digital Object Identifier (DOI)https://doi.org/10.3390/ijms242316757
Open accessPublished as ‘gold’ (paid) open access
FunderBiotechnology and Biological Sciences Research Council
Funder project or codeDesigning Future Wheat - WP1 - Increased efficiency and sustainability
Publisher's version
Output statusPublished
Publication dates
Online25 Nov 2023
Publication process dates
Accepted23 Nov 2023
PublisherMDPI
ISSN1422-0067

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