Nigel Halford

NameNigel Halford
Job titlePrincipal Research Scientist - Crop Science
Email addressnigel.halford@rothamsted.ac.uk
DepartmentSustainable Soils and Crops
Research clusterPS: Resilience and Development
ORCIDhttps://orcid.org/0000-0001-6488-2530
Preferred citationHalford, N. G.
OfficeHarpenden

Research outputs

Low asparagine wheat - Europe’s first field trial of genome edited wheat amid rapidly changing regulations on acrylamide in food and genome editing of crops

Kaur, N., Brock, T., Raffan, S. and Halford, N. G. 2023. Low asparagine wheat - Europe’s first field trial of genome edited wheat amid rapidly changing regulations on acrylamide in food and genome editing of crops. Breeding Science. https://doi.org/10.1270/jsbbs.23058

Transcriptomic and Metabolomic Analyses Reveal the Importance of Lipid Metabolism and Photosynthesis Regulation in High Salinity Tolerance in Barley (Hordeum vulgare L.) Leaves Derived from Mutagenesis Combined with Microspore Culture

Xu, H., Halford, N. G., Guo, G., Chen, Z., Li, Y., Zhou, L, Liu, C. and Xu, R. 2023. Transcriptomic and Metabolomic Analyses Reveal the Importance of Lipid Metabolism and Photosynthesis Regulation in High Salinity Tolerance in Barley (Hordeum vulgare L.) Leaves Derived from Mutagenesis Combined with Microspore Culture. International Journal Of Molecular Sciences. 24 (23), p. 16757. https://doi.org/10.3390/ijms242316757

Reducing the risk of acrylamide and other processing contaminant formation in wheat products

Kaur, N. and Halford, N. G. 2023. Reducing the risk of acrylamide and other processing contaminant formation in wheat products . Foods. 12 (17), p. 3264. https://doi.org/10.3390/foods12173264

Real-Time Quantitative PCR - Primer Design, Reference Gene Selection, Calculations and Statistics

Chen, Z., Halford, N. G. and Liu, C. 2023. Real-Time Quantitative PCR - Primer Design, Reference Gene Selection, Calculations and Statistics. Metabolites. 13 (7), p. 806. https://doi.org/10.3390/metabo13070806

Uncovering plant epigenetics - new insights into cytosine methylation in rye genomes

Kaur, N., Nayakoti, S., Brock, T. and Halford, N. G. 2023. Uncovering plant epigenetics - new insights into cytosine methylation in rye genomes . Journal of Experimental Botany. 74 (12), pp. 3395-3398. https://doi.org/10.1093/jxb/erad144

Genetic control of grain amino acid composition in a UK soft wheat mapping population

Oddy, J., Chhetry, M., Awal, R., Addy, J., Wilkinson, M. D., Smith, D., King, R., Hall, C., Testa, R., Murray, E., Raffan, S., Curtis, T. Y., Wingen, L., Griffiths, S., Berry, S., Elmore, S. J., Cryer, N., Moreira de Almeida, I. and Halford, N. G. 2023. Genetic control of grain amino acid composition in a UK soft wheat mapping population. The Plant Genome. 16 (4), p. e20335. https://doi.org/10.1002/tpg2.20335

Accounting for environmental variation in the free asparagine content of wheat grain

Oddy, J., Elmore, S. and Halford, N. G. 2023. Accounting for environmental variation in the free asparagine content of wheat grain. Journal of Food Composition and Analysis - JFCA. 120, p. 105333. https://doi.org/10.1016/j.jfca.2023.105333

Field assessment of genome edited, low asparagine wheat: Europe's first CRISPR wheat field trial.

Raffan, S., Oddy, J., Mead, A., Barker, G., Curtis, T. Y., Usher, S. L., Burt, C. and Halford, N. G. 2023. Field assessment of genome edited, low asparagine wheat: Europe's first CRISPR wheat field trial. Plant Biotechnology Journal. 21 (6), pp. 1097-1099. https://doi.org/10.1111/pbi.14026

Reducing dietary acrylamide exposure from wheat products through crop management and imaging

Oddy, J., Addy, J., Mead, A., Hall, C., Mackay, C., Ashfield, T., Mcdiarmid, F., Curtis, T. Y., Raffan, S., Wilkinson, M. D., Elmore, J. S., Cryer, N., Moreira de Almeida, I. and Halford, N. G. 2023. Reducing dietary acrylamide exposure from wheat products through crop management and imaging. Journal of Agricultural and Food Chemistry - JAFC. 71 (7), pp. 3403-3413. https://doi.org/10.1021/acs.jafc.2c07208

Genetic diversity and population structure analysis of barley landraces from Shanghai region using genotyping-by-sequencing

Li, L., Halford, N. G., Wang, H., Zong, Y., Guo, Z., Lu, R., Liu, C. and Chen, Z. 2023. Genetic diversity and population structure analysis of barley landraces from Shanghai region using genotyping-by-sequencing. Phyton. 92 (4), pp. 1275-1287. https://doi.org/10.32604/phyton.2023.026946

Epigenetic switch reveals CRISPR/Cas9 response to cytosine methylation in plants: Comment

Raffan, S., Kaur, N. and Halford, N. G. 2022. Epigenetic switch reveals CRISPR/Cas9 response to cytosine methylation in plants: Comment. New Phytologist. 235 (6), pp. 2146-2148. https://doi.org/10.1111/nph.18405

Advances in barley breeding for improving nitrogen use efficiency

Chen, Z., Li, L., Halford, N. G., Xu, H., Huang, L., Gao, R., Lu, R. and Liu, C 2022. Advances in barley breeding for improving nitrogen use efficiency. Agronomy. 12 (7), p. 1682. https://doi.org/10.3390/agronomy12071682

Global wheat production could benefit from closing the genetic yield gap

Senapati, N., Semenov, M. A., Halford, N. G., Hawkesford, M. J., Asseng, S., Cooper, M., Ewert, F., Ittersum, M. K. V., Martre, P., Olesen, J. E., Reynolds, M., Rotter, R. P. and Webber, H. 2022. Global wheat production could benefit from closing the genetic yield gap. Nature Food. 3 (July), pp. 532-541. https://doi.org/10.1038/s43016-022-00540-9

Progress towards the production of potatoes and cereals with low acrylamide-forming potential

Halford, N. G., Raffan, S. and Oddy, J. 2022. Progress towards the production of potatoes and cereals with low acrylamide-forming potential. Current Opinion in Food Science. 47, p. 100887. https://doi.org/10.1016/j.cofs.2022.100887

Understanding the Relationships between Free Asparagine in Grain and Other Traits to Breed Low-Asparagine Wheat

Oddy, J., Raffan, S., Wilkinson, M. D., Elmore, J. S. and Halford, N. G. 2022. Understanding the Relationships between Free Asparagine in Grain and Other Traits to Breed Low-Asparagine Wheat. Plants - Basel. 11 (5), p. 669. https://doi.org/10.3390/plants11050669

Phosphorylation of the alpha-subunit of plant eukaryotic initiation factor 2 prevents its association with polysomes but does not considerably suppress protein synthesis

Zhigailov, A. V., Stanbekova, G. E., Nizkorodova, A. S., Galiakparova, N. N., Gritsenko, D. A., Polimbetova, N. S., Halford, N. G. and Iskakov, B. K. 2022. Phosphorylation of the alpha-subunit of plant eukaryotic initiation factor 2 prevents its association with polysomes but does not considerably suppress protein synthesis. Plant Science. 317, p. 111190. https://doi.org/10.1016/j.plantsci.2022.111190

My lab unlocked: Professor Nigel Halford FRSB

Halford, N. G. 2021. My lab unlocked: Professor Nigel Halford FRSB. The Biologist.

Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency than Parental Lines by F1 Microspore Embryogenesis

Xu, H., Li, Y., Gao, R., Xu, R., Guo, G., Lu, R., Halford, N. G., Chen, Z. and Liu, C. 2021. Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency than Parental Lines by F1 Microspore Embryogenesis. Plants - Basel. 10 (8), p. 1588. https://doi.org/10.3390/plants10081588

Reduced free asparagine in wheat grain resulting from a natural deletion of TaASNB2: investigating and exploiting diversity in the asparagine synthetase gene family to improve wheat quality

Oddy, J., Alarcon-Reverte, R., Wilkinson, M. D., Ravet, K., Raffan, S., Minter, A., Mead, A., Elmore, J. S., Moreira de Almeida, I., Cryer, N., Halford, N. G. and Pearce, S. 2021. Reduced free asparagine in wheat grain resulting from a natural deletion of TaASNB2: investigating and exploiting diversity in the asparagine synthetase gene family to improve wheat quality. BMC Plant Biology. 21 (1), p. 302. https://doi.org/10.1186/s12870-021-03058-7

Genome-wide identification of sucrose nonfermenting-1-related protein kinase (SnRK) genes in barley and RNA-seq analyses of their expression in response to ABA treatment

Chen, Z., Zhou, L., Jiang, P., Lu, R., Halford, N. G. and Liu, C. 2021. Genome-wide identification of sucrose nonfermenting-1-related protein kinase (SnRK) genes in barley and RNA-seq analyses of their expression in response to ABA treatment . BMC Genomics. 22 (article), p. 300. https://doi.org/10.1186/s12864-021-07601-6

Progress on reducing acrylamide levels in potato crisps in Europe, 2002 to 2019

Powers, S. J., Mottram, D. S., Curtis, A. and Halford, N. G. 2021. Progress on reducing acrylamide levels in potato crisps in Europe, 2002 to 2019. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment. 38 (5), pp. 782-806. https://doi.org/10.1080/19440049.2020.1871083

Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2

Raffan, S., Sparks, C. A., Huttly, A. K., Hyde, L., Martignago, D., Mead, A., Hanley, S. J., Wilkinson, P., Barker, G., Edwards, K. J., Curtis, T. Y., Usher, S. L., Kosik, O. and Halford, N. G. 2021. Wheat with greatly reduced accumulation of free asparagine in the grain, produced by CRISPR/Cas9 editing of asparagine synthetase gene TaASN2 . Plant Biotechnology Journal. 19 (8), pp. 1602-1613. https://doi.org/10.1111/pbi.13573

Vulnerability of European wheat to extreme heat and drought around flowering under future climate

Senapati, N., Halford, N. G. and Semenov, M. A. 2021. Vulnerability of European wheat to extreme heat and drought around flowering under future climate. Environmental Research Letters. 16 (2), p. 024052. https://doi.org/10.1088/1748-9326/abdcf3

Cereal asparagine synthetase genes

Raffan, S. and Halford, N. G. 2020. Cereal asparagine synthetase genes. Annals of Applied Biology - AAB. https://doi.org/10.1111/aab.12632

Stress, nutrients and genotype - understanding and managing asparagine accumulation in wheat grain.

Oddy, J., Raffan, S., Wilkinson, M. D., Elmore, J. S. and Halford, N. G. 2020. Stress, nutrients and genotype - understanding and managing asparagine accumulation in wheat grain. CABI Agriculture and Bioscience. 1, p. 10. https://doi.org/10.1186/s43170-020-00010-x

Evidence that phosphorylation of the alpha-subunit of eIF2 does not essentially inhibit mRNA translation in wheat germ cell-free system.

Zhigailov, A. V., Alexandrova, A. M., Nizkorodova, A. S., Stanbekova, G. E., Kryldakov, R. V., Karpova, O. V., Polimbetova, N. S., Halford, N. G. and Iskakov, B. K. 2020. Evidence that phosphorylation of the alpha-subunit of eIF2 does not essentially inhibit mRNA translation in wheat germ cell-free system. Frontiers in Plant Science. 11, p. 936. https://doi.org/10.3389/fpls.2020.00936

The sulphur response in wheat and its implications for acrylamide formation and food safety

Raffan, S., Oddy, J. and Halford, N. G. 2020. The sulphur response in wheat and its implications for acrylamide formation and food safety. International Journal Of Molecular Sciences. 21 (11), p. 3876. https://doi.org/10.3390/ijms21113876

Novel low-nitrogen stress-responsive long non-coding RNAs (lncRNA) in barley landrace B968 (Liuzhutouzidamai) at seedling stage

Chen, Z., Jiang, Q., Jiang, P., Zhang, W, Huang, J., Liu, C, Halford, N. G. and Lu, R. 2020. Novel low-nitrogen stress-responsive long non-coding RNAs (lncRNA) in barley landrace B968 (Liuzhutouzidamai) at seedling stage. BMC Plant Biology. 20, p. 142. https://doi.org/10.1186/s12870-020-02350-2

Top of the Crops. Has the EU got it wrong on genetically modified and gene edited crops?

Halford, N. G. 2020. Top of the Crops. Has the EU got it wrong on genetically modified and gene edited crops? The Actuary. January/February 2020, pp. 20-21.

Research on the genetic control of flowering in potato set to blossom - The control of flowering in potato

Halford, N. G. 2019. Research on the genetic control of flowering in potato set to blossom - The control of flowering in potato. Journal of Experimental Botany. 71 (3), pp. 747-748. https://doi.org/10.1093/jxb/erz544

The wheat SnRK1α family and its contribution to Fusarium toxin tolerance.

Perochon, A., Vary, Z., Malla, K. B., Halford, N. G., Paul, M. J. and Doohan, F. M. 2019. The wheat SnRK1α family and its contribution to Fusarium toxin tolerance. Plant Science. 288, p. 110217. https://doi.org/10.1016/j.plantsci.2019.110217

Acrylamide in food: progress in and prospects for genetic and agronomic solutions

Raffan, S. and Halford, N. G. 2019. Acrylamide in food: progress in and prospects for genetic and agronomic solutions. Annals of Applied Biology - AAB. 175 (3), pp. 259-281. https://doi.org/10.1111/aab.12536

Contrasting gene expression patterns in grain of high and low asparagine wheat genotypes in response to sulphur supply

Curtis, T., Raffan, S., Wan, Y., King, R., Gonzalez-Uriarte, A. and Halford, N. G. 2019. Contrasting gene expression patterns in grain of high and low asparagine wheat genotypes in response to sulphur supply. BMC Genomics. 20, p. 628. https://doi.org/10.1186/s12864-019-5991-8

Acrylamide in Food

Halford, N. G. and Curtis, T. 2019. Acrylamide in Food. World Sci Publ Co Inc.

Acrylamide: new European risk management measures and prospects for reducing the acrylamide-forming potential of wheat

Halford, N. G. and Raffan, S. 2019. Acrylamide: new European risk management measures and prospects for reducing the acrylamide-forming potential of wheat. in: Food-Borne Toxicants: Formation, Analysis, and Toxicology Washington DC American Chemical Society (ACS). pp. Chapter 3, pp 27–43

Reducing the acrylamide-forming potential of crop plants

Raffan, S. and Halford, N. G. 2019. Reducing the acrylamide-forming potential of crop plants. in: Jaiwal, P. K., Chhillar, A. K., Chaudhary, D. and Jaiwal, R. (ed.) Nutritional quality improvement in plants Basel, Switzerland Springer Nature.

Crop Biotechnology: Genetic Modification and Genome Editing

Halford, N. G. 2018. Crop Biotechnology: Genetic Modification and Genome Editing. London World Scientific Publ Co Pte Ltd.

Legislation governing genetically modified (GM) and genome edited crops in Europe: The need for change

Halford, N. G. 2019. Legislation governing genetically modified (GM) and genome edited crops in Europe: The need for change. Journal of the Science of Food and Agriculture. 99 (1), pp. 8-12. https://doi.org/10.1002/jsfa.9227

Reference gene selection for quantitative RT-PCR normalisation in barley under low nitrogen

Chen, Z., Li, Y., Liu, C., Wang, Y., He, T., Guo, G., Fang, C., Gao, R., Xu, H., Zhou, L., Huang, J., Halford, N. G. and Lu, R. 2018. Reference gene selection for quantitative RT-PCR normalisation in barley under low nitrogen. Journal of Cereal Science. 82 (July), pp. 213-215. https://doi.org/10.1016/j.jcs.2018.06.014

Managing Acrylamide at the Agricultural Stage: Variety Selection, Crop Management, and the Prospects for Solving the Acrylamide Problem Through Plant Breeding and Biotechnology

Halford, N. G. 2018. Managing Acrylamide at the Agricultural Stage: Variety Selection, Crop Management, and the Prospects for Solving the Acrylamide Problem Through Plant Breeding and Biotechnology. in: Encyclopedia of Food Chemistry, Volume 1 Elsevier. pp. 559–568

Construction of a network describing asparagine metabolism in plants and its application to the identification of genes affecting asparagine metabolism in wheat under drought and nutritional stress

Curtis, T. Y., Bo, V., Tucker A. and Halford, N. G. 2018. Construction of a network describing asparagine metabolism in plants and its application to the identification of genes affecting asparagine metabolism in wheat under drought and nutritional stress. Food and Energy Security. 7 (1), p. e00126. https://doi.org/10.1002/fes3.126

Genomic, biochemical and modelling analyses of asparagine synthetases from wheat

Xu, H., Curtis, T. Y., Powers, S. J., Raffan, S., Gao, R., Huang, J., Heiner, M., Gilbert, D. R. and Halford, N. G. 2018. Genomic, biochemical and modelling analyses of asparagine synthetases from wheat. Frontiers in Plant Science. 8, p. 2237. https://doi.org/10.3389/fpls.2017.02237

Europe tightens the screw on acrylamide in food

Halford, N. G. 2017. Europe tightens the screw on acrylamide in food. Food & Beverage News.

Triticum aestivum cultivar Spark asparagine synthetase-1 (ASN1) mRNA, complete cds

Xu, H., Curtis, T. Y., Powers, S. J., Raffan, S., Gao, R., Huang, J., Heiner, M., Gilbert, D. and Halford, N. G. 2017. Triticum aestivum cultivar Spark asparagine synthetase-1 (ASN1) mRNA, complete cds.

Secale cereale glutamine synthetase-1 (GS1-1) mRNA, partial cds

Curtis, T., Halford, N. G. and Raffan, S. 2017. Secale cereale glutamine synthetase-1 (GS1-1) mRNA, partial cds.

Triticum aestivum cultivar Spark asparagine synthetase-2 (ASN2) mRNA, complete cds

Halford, N. G. 2017. Triticum aestivum cultivar Spark asparagine synthetase-2 (ASN2) mRNA, complete cds.

Triticum aestivum cultivar Spark asparagine synthetase-3 (ASN3) mRNA, complete cds

Xu, H., Curtis, T. Y., Powers, S. J., Raffan, S., Gao, R., Huang, J., Heiner, M., Gilbert, D. and Halford, N. G. 2017. Triticum aestivum cultivar Spark asparagine synthetase-3 (ASN3) mRNA, complete cds.

Acrylamide levels in potato crisps in Europe from 2002 to 2016

Powers, S. J., Curtis, A, Mottram, D. S. and Halford, N. G. 2017. Acrylamide levels in potato crisps in Europe from 2002 to 2016. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment. 34 (12), pp. 2085-2100. https://doi.org/10.1080/19440049.2017.1379101

Effects of variety, year of cultivation and sulphur supply on the accumulation of free asparagine in the grain of commercial wheat varieties

Curtis, T., Powers, S. J., Wang, R. and Halford, N. G. 2018. Effects of variety, year of cultivation and sulphur supply on the accumulation of free asparagine in the grain of commercial wheat varieties. Food Chemistry. 239 (15 January), pp. 304-313. https://doi.org/10.1016/j.foodchem.2017.06.113

Effects of fungicide treatment on free amino acid concentration and acrylamide-forming potential in wheat

Curtis, T. Y., Powers, S. J. and Halford, N. G. 2016. Effects of fungicide treatment on free amino acid concentration and acrylamide-forming potential in wheat. Journal of Agricultural and Food Chemistry - JAFC. 64 (51), pp. 9689-9696. https://doi.org/10.1021/acs.jafc.6b04520

Relationship between Alkylpyrazine and Acrylamide Formation in Potato Chips

Elmore, J. S., Snowden, S., Briddon, A., Halford, N. G. and Mottram, D. S. 2016. Relationship between Alkylpyrazine and Acrylamide Formation in Potato Chips. in: Granvogl, S. (ed.) Browned Flavors: Analysis, Formation, & Physiology Washington DC American Chemical Society (ACS). pp. 133-144

Reducing the Acrylamide-Forming Potential of Wheat, Rye and Potato: A Review [Chapter 4]

Halford, N. G. and Curtis, T. Y. 2016. Reducing the Acrylamide-Forming Potential of Wheat, Rye and Potato: A Review [Chapter 4]. in: Granvogl, M., Peterson, D. and Schieberle, P. (ed.) Browned Flavors: Analysis, Formation, & Physiology Washington DC American Chemical Society (ACS). pp. 35-53

Genomic advancement: aiming to affirm and improve human life

Barash, C. I. and Halford, N. G. 2016. Genomic advancement: aiming to affirm and improve human life. Applied and Translational Genomics. 11 (December), pp. 1-2. https://doi.org/10.1016/j.atg.2016.11.002

Acrylamide-forming potential of potatoes grown at different locations, and the ratio of free asparagine to reducing sugars at which free asparagine becomes a limiting factor for acrylamide formation

Muttucumaru, N., Powers, S. J., Elmore, J. S., Dodson, A., Briddon, A., Mottram, D. S. and Halford, N. G. 2017. Acrylamide-forming potential of potatoes grown at different locations, and the ratio of free asparagine to reducing sugars at which free asparagine becomes a limiting factor for acrylamide formation. Food Chemistry. 220 (1 April), pp. 76-86. https://doi.org/10.1016/j.foodchem.2016.09.199

Changes in free amino acid concentration in rye grain in response to nitrogen and sulphur availability, and expression analysis of genes involved in asparagine metabolism

Postles, J., Curtis, T. Y., Powers, S. J., Elmore, J. S., Mottram, D. S. and Halford, N. G. 2016. Changes in free amino acid concentration in rye grain in response to nitrogen and sulphur availability, and expression analysis of genes involved in asparagine metabolism. Frontiers in Plant Science. 7, p. 917. https://doi.org/10.3389/fpls.2016.00917

Reducing the acrylamide-forming potential of wheat

Curtis, T. Y. and Halford, N. G. 2016. Reducing the acrylamide-forming potential of wheat. Food and Energy Security. 5 (3), pp. 153-164. https://doi.org/10.1002/fes3.85

Food safety: structure and expression of the asparagine synthetase gene family of wheat

Gao, R., Curtis, T. Y., Powers, S. J., Xu, H., Huang, J. and Halford, N. G. 2016. Food safety: structure and expression of the asparagine synthetase gene family of wheat. Journal of Cereal Science. 68, pp. 122-131. https://doi.org/10.1016/j.jcs.2016.01.010

Acrylamide in cereals: the problem, and potential genetic and agronomic solutions

Halford, N. G. and Curtis, T. Y. 2016. Acrylamide in cereals: the problem, and potential genetic and agronomic solutions. in: Jones, H. D. (ed.) Biotechnology of major cereals Wallingford CAB International (CABI). pp. 165-178

Producing a road map that enables plants to cope with future climate change

Halford, N. G. and Foyer, C. H. 2015. Producing a road map that enables plants to cope with future climate change. Journal of Experimental Botany. 66 (12), pp. 3433-3434. https://doi.org/10.1093/jxb/erv277

An introduction to bioenergy

Halford, N. G. 2015. An introduction to bioenergy. Imperial College Press, London.

Acrylamide in potato crisps prepared from 20 UK-grown varieties: effects of variey and tuber storage time

Elmore, J. S., Briddon, A., Dodson, A. T., Muttucumaru, N., Halford, N. G. and Mottram, D. S. 2015. Acrylamide in potato crisps prepared from 20 UK-grown varieties: effects of variey and tuber storage time. Food Chemistry. 182, pp. 1-8. https://doi.org/10.1016/j.foodchem.2015.02.103

Effects of water availability on free amino acids, sugars and acrylamide-forming potential in potato

Muttucumaru, N., Powers, S. J., Elmore, J. S., Mottram, D. S. and Halford, N. G. 2015. Effects of water availability on free amino acids, sugars and acrylamide-forming potential in potato. Journal of Agricultural and Food Chemistry - JAFC. 63, pp. 2566-2575. https://doi.org/10.1021/jf506031w

Effects of abiotic stress and crop management on cereal grain composition: implications for food quality and safety

Halford, N. G., Curtis, T. Y., Chen, Z. and Huang, J. 2015. Effects of abiotic stress and crop management on cereal grain composition: implications for food quality and safety. Journal of Experimental Botany. 66, pp. 1145-1156. https://doi.org/10.1093/jxb/eru473

Genetically modified plants

Halford, N. G. 2014. Genetically modified plants. in: eLS Chichester, West Sussex John Wiley & Sons Ltd.

Effect of sulphur fertilisation on the acrylamide-forming potential of wheat (HGCA Project Report No. 525)

Curtis, T., Halford, N. G., Powers, S. J., McGrath, S. P. and Zazzeroni, R. 2014. Effect of sulphur fertilisation on the acrylamide-forming potential of wheat (HGCA Project Report No. 525). Home Grown Cereals Authority (HGCA) Stoneleigh.

Safety assessment of genetically modified plants with deliberately altered composition

Halford, N. G., Hudson, E., Gimson, A., Weightman, R., Shewry, P. R. and Tompkins, S. 2014. Safety assessment of genetically modified plants with deliberately altered composition. Plant Biotechnology Journal. 12 (6), pp. 651-654. https://doi.org/10.1111/pbi.12194

Reducing the potential for processing contaminant formation in cereal products

Curtis, T. Y., Postles, J. and Halford, N. G. 2014. Reducing the potential for processing contaminant formation in cereal products. Journal of Cereal Science. 59, pp. 382-392. https://doi.org/10.1016/j.jcs.2013.11.002

Food security: the challenge of increasing wheat yield and the importance of not compromising food safety

Curtis, T. Y. and Halford, N. G. 2014. Food security: the challenge of increasing wheat yield and the importance of not compromising food safety. Annals of Applied Biology - AAB. 164 (3), pp. 354-372. https://doi.org/10.1111/aab.12108

Evidence for the complex relationship between free amino acid and sugar concentrations and acrylamide-forming potential in potato

Muttucumaru, N., Powers, S. J., Elmore, J. S., Briddon, A., Mottram, D. S. and Halford, N. G. 2014. Evidence for the complex relationship between free amino acid and sugar concentrations and acrylamide-forming potential in potato. Annals of Applied Biology - AAB. 164 (2), pp. 286-300. https://doi.org/10.1111/aab.12101

Photosynthetic assimilation of 14C into amino acids in potato (Solanum tuberosum) and asparagine in the tubers

Muttucumaru, N., Keys, A. J., Parry, M. A. J., Powers, S. J. and Halford, N. G. 2014. Photosynthetic assimilation of 14C into amino acids in potato (Solanum tuberosum) and asparagine in the tubers. Planta. 239, pp. 161-170. https://doi.org/10.1007/s00425-013-1967-0

Food chemistry : A Kazakhstan perspective on the Maillard reaction and acrylamide formation in common foods

Halford, N. G. 2013. Food chemistry : A Kazakhstan perspective on the Maillard reaction and acrylamide formation in common foods. Eurasian Chemico-Technological Journal.

Effects of variety and nutrient availability on the acrylamide forming potential of rye

Postles, J., Elmore, J. S., Powers, S. J., Mottram, D. S. and Halford, N. G. 2013. Effects of variety and nutrient availability on the acrylamide forming potential of rye. Acrylamide, furans and other food-borne contaminants, from plant science to food chemistry. Aspects of Applied Biology 116 . pp. 125-130

Activity of enzymes associated with asparagine synthesis and breakdown in wheat under biotic and abiotic stress conditions

Kudiyarova, Z. S., Muttucumaru, N., West, J. S. and Halford, N. G. 2013. Activity of enzymes associated with asparagine synthesis and breakdown in wheat under biotic and abiotic stress conditions. Acrylamide, furans and other food-borne contaminants, from plant science to food chemistry. Aspects of Applied Biology 116 . pp. 119-124

Producing low acrylamide risk potatoes: a three-year public/private sector collaborative project in the United Kingdom, focussed on genetics, agronomy and storage

Halford, N. G. and Muttucumaru, N. 2013. Producing low acrylamide risk potatoes: a three-year public/private sector collaborative project in the United Kingdom, focussed on genetics, agronomy and storage. Acrylamide, furans and other food-borne contaminants, from plant science to food chemistry. Aspects of Applied Biology 116 . pp. 93-96

Acrylamide, furans and other food-borne contaminants, from plant science to food chemistry

Halford, N. G. (ed.) 2013. Acrylamide, furans and other food-borne contaminants, from plant science to food chemistry. Association of Applied Biologists (AAB) Wellesbourne Warwick .

Acrylamide and flavour in potato crisps

Elmore, J. S., Halford, N. G., Briddon, A., Dodson, A. T., Muttucumaru, N. and Mottram, D. S. 2013. Acrylamide and flavour in potato crisps.

Advances in reduction of acrylamide-forming potential in wheat

Curtis, T. Y. and Halford, N. G. 2013. Advances in reduction of acrylamide-forming potential in wheat. Acrylamide, furans and other food-borne contaminants, from plant science to food chemistry. Aspects of Applied Biology 116 . pp. 131-136

Expression analysis of abscisic acid (ABA) and metabolic signalling factors in developing endosperm and embryo of barley

Chen, Z., Huang, J., Muttucumaru, N., Powers, S. J. and Halford, N. G. 2013. Expression analysis of abscisic acid (ABA) and metabolic signalling factors in developing endosperm and embryo of barley. Journal of Cereal Science. 58, pp. 255-262. https://doi.org/10.1016/j.jcs.2013.06.009

Review of the strategies for the comprehensive food and feed safety and nutritional assessment of GM plants per se. EFSA supporting publication 2013: EN-480

Tompkins, S., Hudson, E., Gimson, A., Halford, N. G., Weightman, R., Shewry, P. R., Wagstaff, C., Commane, D. and Lyne, A. 2013. Review of the strategies for the comprehensive food and feed safety and nutritional assessment of GM plants per se. EFSA supporting publication 2013: EN-480. European Food Safety Authority, Parma.

Acrylamide concentrations in potato crisps in Europe from 2002 to 2011

Powers, S. J., Mottram, D. S., Curtis, A. and Halford, N. G. 2013. Acrylamide concentrations in potato crisps in Europe from 2002 to 2011. Food Additives and Contaminants. 30 (9), pp. 1493-1500. https://doi.org/10.1080/19440049.2013.805439

Effects of nitrogen and sulfur fertilization on free amino acids, sugars and acrylamide-forming potential in potato

Muttucumaru, N., Powers, S. J., Elmore, J. S., Mottram, D. S. and Halford, N. G. 2013. Effects of nitrogen and sulfur fertilization on free amino acids, sugars and acrylamide-forming potential in potato. Journal of Agricultural and Food Chemistry - JAFC. 61, pp. 6734-6742. https://doi.org/10.1021/jf401570x

Effects of variety and nutrient availability on the acrylamide-forming potential of rye grain

Postles, J., Powers, S. J., Elmore, J. S., Mottram, D. S. and Halford, N. G. 2013. Effects of variety and nutrient availability on the acrylamide-forming potential of rye grain. Journal of Cereal Science. 57, pp. 463-470. https://doi.org/10.1016/j.jcs.2013.02.001

Concentrations of free amino acids and sugars in nine potato varieties: effects of storage and relationship with acrylamide formation

Halford, N. G., Muttucumaru, N., Powers, S. J., Gillatt, P. N., Hartley, L., Elmore, J. S. and Mottram, D. S. 2012. Concentrations of free amino acids and sugars in nine potato varieties: effects of storage and relationship with acrylamide formation. Journal of Agricultural and Food Chemistry - JAFC. 60, pp. 12044-12055. https://doi.org/10.1021/jf3037566

Toward two decades of plant biotechnology: successes, failures and prospects

Halford, N. G. 2012. Toward two decades of plant biotechnology: successes, failures and prospects. Food and Energy Security. 1, pp. 9-28. https://doi.org/10.1002/fes3.3

The acrylamide problem: a plant and agronomic science issue

Halford, N. G., Curtis, T. Y., Muttucumaru, N., Postles, J., Elmore, J. S. and Mottram, D. S. 2012. The acrylamide problem: a plant and agronomic science issue. Journal of Experimental Botany. 63, pp. 2841-2851. https://doi.org/10.1093/jxb/ers011

Genetically modified crops, 2nd ed

Halford, N. G. 2012. Genetically modified crops, 2nd ed. Imperial College Press, London.

Evidence that abscisic acid promotes degradation of SNF1-related protein kinase (SnRK) 1 in wheat and activation of a putative calcium-dependent SnRK2

Coello, P., Hirano, E., Hey, S. J., Muttucumaru, N., Martinez-Barajas, E., Parry, M. A. J. and Halford, N. G. 2012. Evidence that abscisic acid promotes degradation of SNF1-related protein kinase (SnRK) 1 in wheat and activation of a putative calcium-dependent SnRK2. Journal of Experimental Botany. 63, pp. 913-924. https://doi.org/10.1093/jxb/err320

Overexpression of GCN2-type protein kinase in wheat has profound effects on free amino acid concentration and gene expression

Byrne, E. H., Prosser, I. M., Muttucumaru, N., Curtis, T. Y., Wingler, A., Powers, S. J. and Halford, N. G. 2012. Overexpression of GCN2-type protein kinase in wheat has profound effects on free amino acid concentration and gene expression. Plant Biotechnology Journal. 10 (3), pp. 328-340. https://doi.org/10.1111/j.1467-7652.2011.00665.x

Challenges and opportunities for using wheat for biofuel production

Shewry, P. R., Freeman, J., Wilkinson, M. D., Pellny, T. K. and Mitchell, R. A. C. 2011. Challenges and opportunities for using wheat for biofuel production. in: Halford, N. G. and Karp, A. (ed.) Energy Crops London Royal Society of Chemistry (RSC). pp. 13-26 Chapter 2

Producing low acrylamide risk potatoes: a three-year public/private sector collaborative project in the United Kingdom, focussed on genetics, agronomy and storage

Halford, N. G. 2011. Producing low acrylamide risk potatoes: a three-year public/private sector collaborative project in the United Kingdom, focussed on genetics, agronomy and storage. Aspects of Applied Biology .

Producing low acrylamide risk potatoes: a three-year public/private sector collaborative project in the United Kingdom, focussed on genetics, agronomy and storage

Halford, N. G. 2011. Producing low acrylamide risk potatoes: a three-year public/private sector collaborative project in the United Kingdom, focussed on genetics, agronomy and storage. In Aspects of Applied Biology .

Systems approaches to crop improvement (Aspects of Applied Biology 107)

Halford, N. G. and Semenov, M. A. (ed.) 2011. Systems approaches to crop improvement (Aspects of Applied Biology 107). Association of Applied Biologists (AAB) Wellesbourne Warwick .

GM crops: from basic research to application

Halford, N. G. and Jones, H. D. (ed.) 2011. GM crops: from basic research to application. Association of Applied Biologists (AAB) Wellesbourne Warwick .

Biomass and energy crops IV (Aspects of Applied Biology 112)

Booth, E., Halford, N. G., Shield, I. F., Taylor, G. and Turley, D. Voigt, T. (ed.) 2011. Biomass and energy crops IV (Aspects of Applied Biology 112). Association of Applied Biologists (AAB) Wellesbourne Warwick .

Willow

Hanley, S. J. 2011. Willow. in: Karp, A. and Halford, N. G. (ed.) Energy crops Royal Society of Chemistry (RSC), Cambridge. pp. 259-274

The role of plant breeding and biotechnology in meeting the challenge of global warming

Halford, N. G. 2011. The role of plant breeding and biotechnology in meeting the challenge of global warming. in: Carayannis, E. G. (ed.) Planet earth 2011 - global warming challenges and opportunities for policy and practice InTechOpen Ltd. pp. 493-506

Sugar beet

May, M. J. 2011. Sugar beet. in: Karp, A. and Halford, N. G. (ed.) Energy crops. RSC Energy and Environment Series No. 3 Royal Society of Chemistry (RSC), Cambridge. pp. 104-115

Evolutionarily recent enzyme system for formation of aspartate and catabolism of glutamate in cereals

Gilmanov, M. K., Kudiyarova, Z. S., Rakhmetova, Z. K., Muttucumaru, N. and Halford, N. G. 2011. Evolutionarily recent enzyme system for formation of aspartate and catabolism of glutamate in cereals. Abstracts Stress Responses: Molecules, Organisms and Environments. Joint Meeting British Ecological Society, Biochemical Society and Society for Experimental Biology, London, 4-7 January 2011 . pp. 40

Sugars in crop plants

Halford, N. G., Curtis, T. Y., Muttucumaru, N., Postles, J. and Mottram, D. S. 2011. Sugars in crop plants. Annals of Applied Biology - AAB. 158 (1), pp. 1-25. https://doi.org/10.1111/j.1744-7348.2010.00443.x

The sucrose non-fermenting-1-related (SnRK) family of protein kinases: potential for manipulation to improve stress tolerance and increase yield

Coello, P., Hey, S. J. and Halford, N. G. 2011. The sucrose non-fermenting-1-related (SnRK) family of protein kinases: potential for manipulation to improve stress tolerance and increase yield. Journal of Experimental Botany. 62, pp. 883-893. https://doi.org/10.1093/jxb/erq331

Energy crops (RSC Energy and Environment Series No 3)

Halford, N. G. and Karp, A. 2011. Energy crops (RSC Energy and Environment Series No 3). Royal Society of Chemistry (RSC), Cambridge.

Challenges and opportunities for using wheat for biofuel production

Shewry, P. R., Freeman, J., Wilkinson, M. D., Pellny, T. K. and Mitchell, R. A. C. 2011. Challenges and opportunities for using wheat for biofuel production. in: Karp, A. and Halford, N. G. (ed.) Energy crops Royal Society of Chemistry (RSC), Cambridge. pp. 13-26

Energy crops: introduction

Karp, A. and Halford, N. G. 2011. Energy crops: introduction. in: Karp, A. and Halford, N. G. (ed.) Energy crops Royal Society of Chemistry (RSC), Cambridge. pp. 1-12

The Maillard reaction in food processing and cooking

Halford, N. G., Curtis, T. Y. and Muttucumaru, N. 2010. The Maillard reaction in food processing and cooking. The Biochemist. 32 (6), pp. 10-14. https://doi.org/10.1042/BIO03206010

The effects of storage on the formation of aroma and acrylamide in heated potato

Halford, N. G. 2010. The effects of storage on the formation of aroma and acrylamide in heated potato. in: In Controlling Maillard Pathways to Generate Flavors

From sugar and amino acid signalling to reducing the potential for acrylamide formation in foods

Halford, N. G., Coello, P., Curtis, T., Hey, S., Muttucumaru, N., Postles, J. and Prosser, I. 2010. From sugar and amino acid signalling to reducing the potential for acrylamide formation in foods. Abstracts GARNet 2010 Meeting, Durham, 6-7 September 2010 .

Acrylamide: influence of plant genetics, agronomy and food processing. Aspects of Applied Biology 97

Halford, N. G. Mottram, D. (ed.) 2010. Acrylamide: influence of plant genetics, agronomy and food processing. Aspects of Applied Biology 97. Association of Applied Biologists (AAB) Wellesbourne Warwick .

Genes and processes controlling amino acid and sugar metabolism in potato: implications for acrylamide forming potential

Muttucumaru, N., Elmore, S., Mottram, D., Parry, M. A. J. and Halford, N. G. 2010. Genes and processes controlling amino acid and sugar metabolism in potato: implications for acrylamide forming potential. Abstracts Society for Experimental Biology Annual Main Meeting, Prague, 30 June-3 July 2010 . pp. 314

Photosynthate partitioning

Halford, N. G. 2010. Photosynthate partitioning. in: Davey, M. R. and Pua, E-C. (ed.) Plant developmental biology - biotechnological perspectives, Vol. 2 Springer, Berlin. pp. 67-82

Effects of sulphur nutrition during potato cultivation on the formation of acrylamide and aroma compounds during cooking

Elmore, J. S., Dodson, A. T., Muttucumaru, N., Halford, N. G., Parry, M. A. J. and Mottram, D. S. 2010. Effects of sulphur nutrition during potato cultivation on the formation of acrylamide and aroma compounds during cooking. Food Chemistry. 122, pp. 753-760. https://doi.org/10.1016/j.foodchem.2010.03.049

Free amino acids and sugars in rye grain: implications for acrylamide formation

Curtis, T. Y., Powers, S. J., Balagiannis, D., Elmore, J. S., Mottram, D. S., Parry, M. A. J., Rakszegi, M., Bedo, Z., Shewry, P. R. and Halford, N. G. 2010. Free amino acids and sugars in rye grain: implications for acrylamide formation. Journal of Agricultural and Food Chemistry - JAFC. 58, pp. 1959-1969. https://doi.org/10.1021/jf903577b

The interface between metabolic and stress signalling

Hey, S. J., Byrne, E. and Halford, N. G. 2010. The interface between metabolic and stress signalling. Annals of Botany. 105, pp. 197-203. https://doi.org/10.1093/aob/mcp285

New insights on the effects of heat stress on crops

Halford, N. G. 2009. New insights on the effects of heat stress on crops. Journal of Experimental Botany. 60 (15), pp. 4215-4216. https://doi.org/10.1093/jxb/erp311

Preface: Special issue on crop science for a changing climate and plant biomass for food and energy

Halford, N. G. 2009. Preface: Special issue on crop science for a changing climate and plant biomass for food and energy. Journal of Experimental Botany. 60 (10), p. 2773. https://doi.org/10.1093/jxb/erp179

ABA response element binding proteins are convergence points for metabolic and stress signalling pathways involving SNF1-related protein kinases

Parry, M. A. J., Hirano, E., Hey, S., Zhang, Y., Andralojc, P. J., Primavesi, L. F. and Halford, N. G. 2009. ABA response element binding proteins are convergence points for metabolic and stress signalling pathways involving SNF1-related protein kinases. Abstracts Plant Biology 2009, Honolulu, 18-22 July 2009 .

Sulphur nutrition differentially affects the distribution of asparagine in wheat grain

Shewry, P. R., Zhao, F-J., Gowa, G. B., Hawkins, N. J., Ward, J. L., Beale, M. H., Halford, N. G., Parry, M. A. J. and Abecassis, J. 2009. Sulphur nutrition differentially affects the distribution of asparagine in wheat grain. Journal of Cereal Science. 50, pp. 407-409. https://doi.org/10.1016/j.jcs.2009.07.001

Identifying target traits and molecular mechanisms for wheat breeding under a changing climate

Semenov, M. A. and Halford, N. G. 2009. Identifying target traits and molecular mechanisms for wheat breeding under a changing climate. Journal of Experimental Botany. 60, pp. 2791-2804. https://doi.org/10.1093/jxb/erp164

Snf1-related protein kinases (SnRKs) act within an intricate network that links metabolic and stress signalling in plants

Halford, N. G. and Hey, S. J. 2009. Snf1-related protein kinases (SnRKs) act within an intricate network that links metabolic and stress signalling in plants. Biochemical Journal. 419, pp. 247-259. https://doi.org/10.1042/BJ20082408

Effects of genotype and environment on free amino acid levels in wheat grain: implications for acrylamide formation during processing

Curtis, T. Y., Muttucumaru, N., Shewry, P. R., Parry, M. A. J., Powers, S. J., Elmore, J. S., Mottram, D. S., Hook, S. and Halford, N. G. 2009. Effects of genotype and environment on free amino acid levels in wheat grain: implications for acrylamide formation during processing. Journal of Agricultural and Food Chemistry - JAFC. 57, pp. 1013-1021. https://doi.org/10.1021/jf8031292

Plant biotechnology: transgenic crops

Shewry, P. R., Jones, H. D. and Halford, N. G. 2008. Plant biotechnology: transgenic crops. in: Stahl, U., Donalies, U. E. B. and Nevoigt, E. (ed.) Food Biotechnology Springer International Publishing Ag.

Effects of climate change on plants: implications for agriculture. Aspects of Applied Biology 88

Halford, N. G., Jones, H. D. and Lawlor, D. W. 2008. Effects of climate change on plants: implications for agriculture. Aspects of Applied Biology 88. Association of Applied Biologists, Wellesbourne.

Arabidopsis sucrose non-fermenting-1-related protein kinase-1 and calcium-dependent protein kinase phosphorylate conserved target sites in ABA response element binding proteins

Zhang, Y., Andralojc, P. J., Hey, S. J., Primavesi, L. F., Specht, M., Koehler, J., Parry, M. A. J. and Halford, N. G. 2008. Arabidopsis sucrose non-fermenting-1-related protein kinase-1 and calcium-dependent protein kinase phosphorylate conserved target sites in ABA response element binding proteins. Annals of Applied Biology - AAB. 153 (3), pp. 401-409. https://doi.org/10.1111/j.1744-7348.2008.00302.x

GCN2-dependent phosphorylation of eukaryotic translation initiation factor 2-alpha in Arabidopsis

Zhang, Y., Wang, Y., Kanyuka, K., Parry, M. A. J., Powers, S. J. and Halford, N. G. 2008. GCN2-dependent phosphorylation of eukaryotic translation initiation factor 2-alpha in Arabidopsis. Journal of Experimental Botany. 59 (11), pp. 3131-3141. https://doi.org/10.1093/jxb/ern169

The wheat GCN2 signalling pathway: does this kinase play an important role in stress signalling?

Byrne, E., Wingler, A., Muttucumaru, N. and Halford, N. G. 2008. The wheat GCN2 signalling pathway: does this kinase play an important role in stress signalling? https://doi.org/10.1016/j.cbpa.2008.04.410

Reducing acrylamide precursors in raw materials derived from wheat and potato

Muttucumaru, N., Elmore, J. S., Curtis, T., Mottram, D. S., Parry, M. A. J. and Halford, N. G. 2008. Reducing acrylamide precursors in raw materials derived from wheat and potato. Journal of Agricultural and Food Chemistry - JAFC. 56, pp. 6167-6172. https://doi.org/10.1021/jf800279d

Effects of plant sulfur nutrition on acrylamide and aroma compounds in cooked wheat

Elmore, J. S., Parker, J. K., Halford, N. G., Muttucumaru, N. and Mottram, D. S. 2008. Effects of plant sulfur nutrition on acrylamide and aroma compounds in cooked wheat. Journal of Agricultural and Food Chemistry - JAFC. 56, pp. 6173-6179. https://doi.org/10.1021/jf0730441

Genetic and agronomic approaches to decreasing acrylamide precursors in crop plants

Halford, N. G., Muttucumaru, N., Curtis, T. Y. and Parry, M. A. J. 2007. Genetic and agronomic approaches to decreasing acrylamide precursors in crop plants. Food Additives and Contaminants. 24(S1), pp. 26-36. https://doi.org/10.1080/02652030701403093

The structure and expression of cereal storage protein genes

Halford, N. G. and Shewry, P. R. 2007. The structure and expression of cereal storage protein genes. in: Olsen, O-A. (ed.) Endosperm: developmental and molecular biology. (Plant Cell Monographs, Vol. 8) Springer, Berlin. pp. 195-218

Changes in free amino acids and sugars in potatoes due to sulfate fertilization and the effect on acrylamide formation

Elmore, J. S., Mottram, D. S., Muttucumaru, N., Dodson, A. T., Parry, M. A. J. and Halford, N. G. 2007. Changes in free amino acids and sugars in potatoes due to sulfate fertilization and the effect on acrylamide formation. Journal of Agricultural and Food Chemistry - JAFC. 55, pp. 5363-5366. https://doi.org/10.1021/jf070447s

DNA sequences from arabidopsis, which encode protein kinases and function as upstream regulators of Snf1 in yeast

Hey, S., Mayerhofer, H., Halford, N. G. and Dickinson, J. R. 2007. DNA sequences from arabidopsis, which encode protein kinases and function as upstream regulators of Snf1 in yeast. Journal of Biological Chemistry. 14, pp. 10472-10479. https://doi.org/10.1074/jbc.M611244200

Asparagine in plants

Lea, P. J., Sodek, L., Parry, M. A. J., Shewry, P. R. and Halford, N. G. 2007. Asparagine in plants. Annals of Applied Biology - AAB. 150 (1), pp. 1-26. https://doi.org/10.1111/j.1744-7348.2006.00104.x

From primitive selection to genetic modification, ten thousand years of plant breeding

Halford, N. G. 2006. From primitive selection to genetic modification, ten thousand years of plant breeding. in: Halford, N. G. (ed.) Plant biotechnology: current and future uses of genetically modified crops John Wiley & Sons Chichester, West Sussex. pp. 3-27

Formation of high levels of acrylamide during the processing of flour derived from sulfate-deprived wheat

Muttucumaru, N., Halford, N. G., Elmore, J. S., Dodson, A. T., Parry, M. A. J., Shewry, P. R. and Mottram, D. S. 2006. Formation of high levels of acrylamide during the processing of flour derived from sulfate-deprived wheat. Journal of Agricultural and Food Chemistry - JAFC. 54, pp. 8951-8955. https://doi.org/10.1021/jf0623081

Kafirin structure and functionality

Belton, P. S., Delgadillo, I., Halford, N. G. and Shewry, P. R. 2006. Kafirin structure and functionality. Journal of Cereal Science. 44, pp. 272-286. https://doi.org/10.1016/j.jcs.2006.05.004

AtGCN2 is involved in response to limitation of amino acid biosynthesis in arabidopsis plants

Zhang, Y., Paul, M. J., Parry, M. A. J. and Halford, N. G. 2006. AtGCN2 is involved in response to limitation of amino acid biosynthesis in arabidopsis plants. Abstracts 8th International Congress of Plant Molecular Biology, Adelaide, 20-25 August 2006 . pp. 217

The role of the protein kinase SnRK1 in regulating carbohydrate metabolism

Halford, N. G., Hey, S., Muttucumaru, N., Zhang, Y., Primavesi, L. F., Jhurreea, D., Andralojc, P. J. and Paul, M. J. 2006. The role of the protein kinase SnRK1 in regulating carbohydrate metabolism. Abstracts 8th International Congress of Plant Molecular Biology, Adelaide, 20-25 August 2006 . pp. 96

Plant food allergens

Mills, E. N. C., Jenkins, J. A. and Shewry, P. R. 2006. Plant food allergens. in: Halford, N. G. (ed.) Plant biotechnology: current and future applications of genetically modified crops John Wiley & Sons Chichester, West Sussex. pp. 243-264

Production of high-starch, low-glucose potatoes through over-expression of the metabolic regulator SnRK1

Mckibbin, R. S., Muttucumaru, N., Paul, M. J., Powers, S. J., Burrell, M. M., Coates, S., Purcell, P. C., Tiessen, A., Geigenberger, P. and Halford, N. G. 2006. Production of high-starch, low-glucose potatoes through over-expression of the metabolic regulator SnRK1. Plant Biotechnology Journal. 4 (4), pp. 409-418. https://doi.org/10.1111/j.1467-7652.2006.00190.x

Nutrient sensing in plant meristems

Francis, D. and Halford, N. G. 2006. Nutrient sensing in plant meristems. Plant Molecular Biology. 60, pp. 981-993. https://doi.org/10.1007/s11103-005-5749-3

The high-molecular-weight subunits of glutenin

Shewry, P. R., Halford, N. G. and Lafiandra, D. 2006. The high-molecular-weight subunits of glutenin. in: Bekes, F., Bushuk, W. and Wrigley, C. (ed.) Gliadin and glutenin: the unique balance of wheat quality American Association of Cereal Chemists (AACC), St Paul, MN. pp. 143-169

Comparison of repetitive sequences derived from high molecular weight subunits of wheat glutenin, an elastomeric plant protein

Wellner, N., Marsh, J. T., Savage, A. W. J., Halford, N. G., Shewry, P. R., Mills, E. N. C. and Belton, P. S. 2006. Comparison of repetitive sequences derived from high molecular weight subunits of wheat glutenin, an elastomeric plant protein. Biomacromolecules. 7, pp. 1096-1103. https://doi.org/10.1021/bm050893t

Regulation of carbon and amino acid metabolism: roles of sucrose nonfermenting-1-related protein kinase-1 and general control nondepressible-2-related protein kinase

Halford, N. G. 2006. Regulation of carbon and amino acid metabolism: roles of sucrose nonfermenting-1-related protein kinase-1 and general control nondepressible-2-related protein kinase. Advances in botanical research. 43, pp. 93-142. https://doi.org/10.1016/S0065-2296(05)43002-5

Is there a future for GM crops in the UK?

Halford, N. G. 2006. Is there a future for GM crops in the UK? Society for Experimental Biology Bulletin. March 2006, pp. 10-11.

The application of genetic engineering to the improvement of cereal grain quality

Shewry, P. R. 2006. The application of genetic engineering to the improvement of cereal grain quality. in: Halford, N. G. (ed.) Plant biotechnology: current and future uses of genetically modified crops John Wiley & Sons Chichester, West Sussex. pp. 133-150

Risk assessment, regulation and labeling

Halford, N. G. 2006. Risk assessment, regulation and labeling. in: Halford, N. G. (ed.) Plant biotechnology: current and future uses of genetically modified crops John Wiley & Sons Chichester, West Sussex. pp. 281-293

Development of biotech crops in China

Xue, Q., Zhang, X. and Zhang, Y. 2006. Development of biotech crops in China. in: Halford, N. G. (ed.) Plant biotechnology: current and future uses of genetically modified crops John Wiley & Sons Chichester, West Sussex. pp. 53-67

Advances in transformation technologies

Jones, H. D. 2006. Advances in transformation technologies. in: Halford, N. G. (ed.) Plant biotechnology: current and future uses of genetically modified crops John Wiley & Sons Chichester, West Sussex. pp. 71-90

Plant Biotechnology: Current and Future Applications of Genetically Modified Crops

Halford, N. G. (ed.) 2006. Plant Biotechnology: Current and Future Applications of Genetically Modified Crops. John Wiley & Sons Chichester, West Sussex.

Enhanced seed phytosterol accumulation through expression of a modified HMG-CoA reductase

Hey, S. J., Powers, S. J., Beale, M. H., Hawkins, N. D., Ward, J. L. and Halford, N. G. 2006. Enhanced seed phytosterol accumulation through expression of a modified HMG-CoA reductase. Plant Biotechnology Journal. 4 (2), pp. 219-229. https://doi.org/10.1111/j.1467-7652.2005.00174.x

Molecular cloning of beta-kafirin, a methionine-rich protein of sorghum grain

Chamba, E. B., Halford, N. G., Forsyth, J., Wilkinson, M. D. and Shewry, P. R. 2005. Molecular cloning of beta-kafirin, a methionine-rich protein of sorghum grain. Journal of Cereal Science. 41 (3), pp. 381-383. https://doi.org/10.1016/j.jcs.2004.09.004

Evidence of diversity within the SnRK1b gene family of Hordeum species

Clark, J. S. C., Dani, M., Halford, N. G. and Karp, A. 2005. Evidence of diversity within the SnRK1b gene family of Hordeum species. Genome. 48 (4), pp. 661-673. https://doi.org/10.1139/g05-024

Comparative analysis of the D genome-encoded high-molecular weight sububits of glutenin

Wan, Y., Yan, Z., Liu, K., Zheng, Y., D'ovidio, R., Shewry, P. R., Halford, N. G. and Wang, D. 2005. Comparative analysis of the D genome-encoded high-molecular weight sububits of glutenin. Theoretical and Applied Genetics. 111, pp. 1183-1190.

Changes in protein secondary structure during gluten deformation studied by dynamic Fourier transform infrared spectroscopy

Wellner, N., Mills, E. N. C., Brownsey, G., Wilson, R. H., Brown, N. A., Freeman, J., Halford, N. G., Shewry, P. R. and Belton, P. S. 2005. Changes in protein secondary structure during gluten deformation studied by dynamic Fourier transform infrared spectroscopy. Biomacromolecules. 6 (1), pp. 255-261. https://doi.org/10.1021/bm049584d

Design, expression and characterisation of lysine-rich forms of the barley seed protein CI-2

Forsyth, J. L., Beaudoin, F., Halford, N. G., Sessions, R. B., Clarke, A. R. and Shewry, P. R. 2005. Design, expression and characterisation of lysine-rich forms of the barley seed protein CI-2. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1747, pp. 221-227. https://doi.org/10.1016/j.bbapap.2004.11.018

Genetic modification of SnRK1 and impact on metabolism in Arabidopsis

Jhurreea, D., Zhang, Y., Andralojc, P. J., Bennett, M., Halford, N. G. and Paul, M. J. 2004. Genetic modification of SnRK1 and impact on metabolism in Arabidopsis.

Structure and biomechanical properties of the gluten proteins deposited in the developing wheat grain: relationship to functional properties at harvest

Shewry, P. R., Wellner, N., Mills, E. N. C., Brownsey, G., Wilson, R. H., Brown, N. A., Freeman, J., Halford, N. G. and Belton, P. S. 2004. Structure and biomechanical properties of the gluten proteins deposited in the developing wheat grain: relationship to functional properties at harvest. Black, C. K., Rebetzke, G. J. and Panozzo, J. F. (ed.) Cereals 2004: Proceedings 54th Australian Cereal Chemistry Conference and 11th Wheat Breeders' Assembly, Canberra, 21-24 September 2004 . Royal Australian Chemical Institute, Melbourne. pp. 377-379

Prospects for genetically modified crops

Halford, N. G. 2004. Prospects for genetically modified crops. Annals of Applied Biology - AAB. 145 (1), pp. 17-24. https://doi.org/10.1111/j.1744-7348.2004.tb00355.x

Use of recombinant peptides to explore the molecular mechanism of gluten protein viscoelasticity

Halford, N. G., Savage, A., Wellner, N., Mills, E. N. C., Belton, P. S. and Shewry, P. R. 2004. Use of recombinant peptides to explore the molecular mechanism of gluten protein viscoelasticity. D'ovidio, R., Lafiandra, D. and Masci, S. (ed.) Gluten Proteins. Proceedings of the 8th Gluten Workshop, Viterbo, 8-10 September 2003 . Royal Society of Chemistry (RSC), Cambridge. pp. 45-57

SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein

Slocombe, S. P., Beaudoin, F., Donaghy, P. G., Hardie, D. G., Dickinson, J. R. and Halford, N. G. 2004. SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein. Plant Physiology and Biochemistry. 42, pp. 111-116.

Highly conserved protein kinases involved in the regulation of carbon and amino acid metabolism

Halford, N. G., Hey, S., Jhurreea, D., Laurie, S., Mckibbin, R. S., Zhang, Y. and Paul, M. J. 2004. Highly conserved protein kinases involved in the regulation of carbon and amino acid metabolism. Journal of Experimental Botany. 55 (1), pp. 35-42. https://doi.org/10.1093/jxb/erh019

The Arabidopsis 14-3-3 protein, GF14omega, binds to the Schizosaccharomyces pombe Cdc25 phosphatase and rescues checkpoint defects in the rad24- mutant

Sorrell, D. A., Marchbank, A. M., Chrimes, D. A., Dickinson, J. R., Rogers, H. J., Francis, D., Grierson, C. S. and Halford, N. G. 2003. The Arabidopsis 14-3-3 protein, GF14omega, binds to the Schizosaccharomyces pombe Cdc25 phosphatase and rescues checkpoint defects in the rad24- mutant. Planta. 218 (1), pp. 50-57. https://doi.org/10.1007/s00425-003-1083-7

The Arabidopsis CDPK-SnRK superfamily of protein kinases

Hrabak, E. M., Chan, C. W. M., Gribskov, M., Harper, J. F., Choi, J. H., Halford, N. G., Kudla, J., Luan, S., Nimmo, H. G., Sussman, M. R., Thomas, M., Walker-Simmons, K., Zhu, J-K. and Harmon, A. C. 2003. The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiology. 132 (2), pp. 666-680. https://doi.org/10.1104/pp.102.011999

Expression of the gamma-zein protein of maize in seeds of transgenic barley: effects on grain composition and properties

Zhang, Y., Darlington, H., Jones, H. D., Halford, N. G., Napier, J. A., Davey, M. R., Lazzeri, P. A. and Shewry, P. R. 2003. Expression of the gamma-zein protein of maize in seeds of transgenic barley: effects on grain composition and properties. Theoretical and Applied Genetics. 106 (6), pp. 1139-1146. https://doi.org/10.1007/s00122-002-1162-3

Regulation of carbon metabolism: sensing, signalling and partitioning. Special issue of the Journal of Experimental Botany, Vol. 54(382)

Halford, N. G. Graham, I. A. (ed.) 2003. Regulation of carbon metabolism: sensing, signalling and partitioning. Special issue of the Journal of Experimental Botany, Vol. 54(382). Oxford University Press (OUP) Oxford.

Prospects for the use of GM crops in the UK

Halford, N. G. 2003. Prospects for the use of GM crops in the UK. Water and Environment Manager. 8(3), p. 3.

The high molecular weight subunits of wheat glutenin and their role in determining wheat processing qualities

Shewry, P. R., Halford, N. G., Tatham, A. S., Popineau, Y., Lafiandra, D. and Belton, P. S. 2003. The high molecular weight subunits of wheat glutenin and their role in determining wheat processing qualities. Advances in Food and Nutrition Research. 45, pp. 219-302.

The prolamin storage proteins of sorghum and millets

Shewry, P. R. and Halford, N. G. 2003. The prolamin storage proteins of sorghum and millets. Belton, P. S. and Taylor, J. R. N. (ed.) Proceedings AFRIPRO Workshop on the Proteins of Sorghum and Millets: Enhancing Nutritional and Functional Properties for Africa, Pretoria, 2-4 April 2003 .

The GM revolution

Holdsworth, M. J. and Halford, N. G. 2003. The GM revolution. in: Shewry, P. R., Lenton, J. R. and Anderson, H. M. (ed.) Long Ashton Research Station: one hundred years of science in support of agriculture Institute for Arable Crops Research (IACR) , Long Ashton Research Station (LARS), Bristol. pp. 312-321

Gluten, the elastomeric protein of wheat seeds

Halford, N. G. and Belton, P. S. 2003. Gluten, the elastomeric protein of wheat seeds. in: Shewry, P. R., Bailey, A. J. and Tatham, A. S. (ed.) Elastomeric proteins: structures, biomechanical properties and biological roles Cambridge University Press (CUP) Cambridge. pp. 279-301

Evidence that SNF1-related kinase and hexokinase are involved in separate sugar-signalling pathways modulating post-translational redox activation of ADP-glucose pyrophosphorylase in potato tubers

Tiessen, A., Prescha, K., Branscheid, A., Palacios, N., Mckibbin, R. S., Halford, N. G. and Geigenbeger, P. 2003. Evidence that SNF1-related kinase and hexokinase are involved in separate sugar-signalling pathways modulating post-translational redox activation of ADP-glucose pyrophosphorylase in potato tubers. The Plant Journal. 35, pp. 490-500.

Genetics of wheat gluten proteins

Shewry, P. R., Halford, N. G. and Lafiandra, D. 2003. Genetics of wheat gluten proteins. Advances in Genetics. 49, pp. 111-184.

Sequence and properties of HMW subunit 1Bx20 from pasta wheat (Triticum durum ) which is associated with poor end use properties

Shewry, P. R., Gilbert, S. M., Savage, A. W. J., Tatham, A. S., Wan, Y., Belton, P. S., Wellner, N., D'ovidio, R., Bekes, F. and Halford, N. G. 2003. Sequence and properties of HMW subunit 1Bx20 from pasta wheat (Triticum durum ) which is associated with poor end use properties. Theoretical and Applied Genetics. 106, pp. 744-750.

Dissection and manipulation of metabolic signalling pathways

Halford, N. G., Hey, S., Jhurreea, D., Laurie, S., Mckibbin, R. S., Zhang, Y. and Paul, M. J. 2003. Dissection and manipulation of metabolic signalling pathways. Annals of Applied Biology - AAB. 142 (1), pp. 25-31. https://doi.org/10.1111/j.1744-7348.2003.tb00225.x

Molecular structures and interactions of repetitive peptides based on wheat glutenin subunits depend on chain length

Feeney, K. A., Wellner, N., Gilbert, S. M., Halford, N. G., Tatham, A. S., Shewry, P. R. and Belton, P. S. 2003. Molecular structures and interactions of repetitive peptides based on wheat glutenin subunits depend on chain length. Biopolymers (Biospectroscopy). 72, pp. 123-131.

Metabolic signalling pathways involved in the regulation of carbon and nitrogen metabolism

Halford, N. G., Hey, S. J., Jhurreea, D., Zhang, Yuhua, Paul, M. J., Laurie, S. and Mckibbin, R. S. 2003. Metabolic signalling pathways involved in the regulation of carbon and nitrogen metabolism. Society for Experimental Biology Annual Main Meeting: 31st March–4th April 2002, Southampton, UK  . https://doi.org/10.1016/S1095-6433(03)00034-5

Metabolic signalling: Role of SnRK1 in regulating sterol biosynthesis

Hey, S. J., Beale, M. H., Mckibbin, R. S., Laurie, S. and Halford, N. G. 2003. Metabolic signalling: Role of SnRK1 in regulating sterol biosynthesis. Society for Experimental Biology Annual Main Meeting: 31st March–4th April 2002, Southampton, UK  . https://doi.org/10.1016/S1095-6433(03)00034-5

Conservation of control mechanisms for regulation of C and N metabolism

Halford, N. G. 2003. Conservation of control mechanisms for regulation of C and N metabolism.

Molecular cloning of an arabidopsis homologue of GCN2, a protein kinase involved in co-ordinated response to amino acid starvation

Zhang, Y., Dickinson, J. R., Paul, M. J. and Halford, N. G. 2003. Molecular cloning of an arabidopsis homologue of GCN2, a protein kinase involved in co-ordinated response to amino acid starvation. Planta. 217, pp. 668-675. https://doi.org/10.1007/s00425-003-1025-4

Carbon metabolite sensing and signalling

Halford, N. G. and Paul, M. J. 2003. Carbon metabolite sensing and signalling. Plant Biotechnology Journal. 1 (6), pp. 381-398. https://doi.org/10.1046/j.1467-7652.2003.00046.x

The case for GM crops

Halford, N. G. 2003. The case for GM crops. Cook School. 2(3).

Genetically modified crops

Halford, N. G. 2003. Genetically modified crops. Imperial College Press, London.

Metabolic signalling and carbon partitioning: role of Snf1-related (SnRK1) protein kinase

Halford, N. G., Hey, S., Jhurreea, D., Laurie, S., Mckibbin, R. S., Paul, M. J. and Zhang, Y. 2003. Metabolic signalling and carbon partitioning: role of Snf1-related (SnRK1) protein kinase. Journal of Experimental Botany. 54, pp. 467-475. https://doi.org/10.1093/jxb/erg038

Antisense SNF1-related (SnRK1) protein kinase gene represses transient activity of an alpha-amylase (alpha-Amy2) gene promoter in cultured wheat embryos

Laurie, S., Mckibbin, R. S. and Halford, N. G. 2003. Antisense SNF1-related (SnRK1) protein kinase gene represses transient activity of an alpha-amylase (alpha-Amy2) gene promoter in cultured wheat embryos. Journal of Experimental Botany. 54, pp. 739-747.

Cereal seed storage proteins: structures, properties and role in grain utilization

Shewry, P. R. and Halford, N. G. 2002. Cereal seed storage proteins: structures, properties and role in grain utilization. Journal of Experimental Botany. 53 (370), pp. 947-958. https://doi.org/10.1093/jexbot/53.370.947

Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica

Wan, Y., Wang, D., Shewry, P. R. and Halford, N. G. 2002. Isolation and characterization of five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Aegilops cylindrica. Theoretical and Applied Genetics. 104, pp. 828-839.

The structure and properties of gluten: an elastic protein from wheat grain

Shewry, P. R., Halford, N. G., Belton, P. S. and Tatham, A. S. 2002. The structure and properties of gluten: an elastic protein from wheat grain. Philosophical Transactions of the Royal Society B-Biological Sciences. 357, pp. 133-142.

Identification of SnIP1, a novel protein that interacts with SNF1-related protein kinase (SnRK1)

Slocombe, S. P., Laurie, S., Bertini, L., Beaudoin, F., Dickinson, J. R. and Halford, N. G. 2002. Identification of SnIP1, a novel protein that interacts with SNF1-related protein kinase (SnRK1). Plant Molecular Biology. 49, pp. 31-44.

The biochemistry and molecular biology of seed storage proteins

Autran, J-C., Halford, N. G. and Shewry, P. R. 2001. The biochemistry and molecular biology of seed storage proteins. in: Lea, P. J. and Morot-Gaudry, J-F. (ed.) Plant nitrogen Springer Verlag, Berlin. pp. 295-341

Sugar sensing and cell cycle control: evidence of cross-talk between two ancient signalling pathways

Halford, N. G. and Dickinson, J. R. 2001. Sugar sensing and cell cycle control: evidence of cross-talk between two ancient signalling pathways. in: Francis, D. (ed.) The plant cell cycle and its interfaces Sheffield Academic Press, Sheffield. pp. 87-107

Development of a novel cloning strategy to investigate the repetitive domain of HMW glutenin subunits

Feeney, K. A., Halford, N. G. and Gilbert, S. M. 2001. Development of a novel cloning strategy to investigate the repetitive domain of HMW glutenin subunits. Shewry, P. R. and Tatham, A. S. (ed.) Wheat Gluten. Proceedings 7th International Workshop Gluten 2000, Bristol, 2-6 April 2000 . Royal Society of Chemistry (RSC), Cambridge. pp. 179-182

Synthesis, expression and characterisation of peptides comprised of perfect repeat motifs based on wheat seed storage protein

Feeney, K. A., Tatham, A. S., Gilbert, S. M., Fido, R. J., Halford, N. G. and Shewry, P. R. 2001. Synthesis, expression and characterisation of peptides comprised of perfect repeat motifs based on wheat seed storage protein. Biochimica et Biophysica Acta (BBA) - Protein structure and molecular enzymology. 1546, pp. 346-355. https://doi.org/10.1016/S0167-4838(01)00155-8

Identification and molecular characterisation of hordoindolines from barley grain

Darlington, H. F., Rouster, J., Hoffmann, L., Halford, N. G., Shewry, P. R. and Simpson, D. J. 2001. Identification and molecular characterisation of hordoindolines from barley grain. Plant Molecular Biology. 47, pp. 785-794.

Cloning of DNA encoding a catalytic subunit of SNF1-related protein kinase-1 (SnRK1-alpha1), and immunological analysis of multiple forms of the kinase, in spinach leaf

Crawford, R. M. M., Halford, N. G. and Hardie, D. G. 2001. Cloning of DNA encoding a catalytic subunit of SNF1-related protein kinase-1 (SnRK1-alpha1), and immunological analysis of multiple forms of the kinase, in spinach leaf. Plant Molecular Biology. 45, pp. 731-741.

Expression of antisense SnRK1 protein kinase sequence causes abnormal pollen development and male sterility in transgenic barley

Zhang, Y., Shewry, P. R., Jones, H. D., Barcelo, P., Lazzeri, P. A. and Halford, N. G. 2001. Expression of antisense SnRK1 protein kinase sequence causes abnormal pollen development and male sterility in transgenic barley. The Plant Journal. 28 (4), pp. 431-441. https://doi.org/10.1046/j.1365-313X.2001.01167.x

Nutritional control of storage protein synthesis in developing grain of wheat and barley

Shewry, P. R., Tatham, A. S. and Halford, N. G. 2001. Nutritional control of storage protein synthesis in developing grain of wheat and barley. Plant Growth Regulation. 34, pp. 105-111.

The role of protein kinases in the regulation of plant growth and development

Laurie, S. and Halford, N. G. 2001. The role of protein kinases in the regulation of plant growth and development. Plant Growth Regulation. 34, pp. 253-265.

Genetic modification and plant food allergens: risks and benefits

Shewry, P. R., Tatham, A. S. and Halford, N. G. 2001. Genetic modification and plant food allergens: risks and benefits. Journal Of Chromatography B. 756 (1/2), pp. 327-335. https://doi.org/10.1016/S0378-4347(01)00093-7

Agricultural biotechnology

Halford, N. G. 2001. Agricultural biotechnology. Chemistry & Industry. 16, pp. 505-507.

Endosperm-specific activity of a storage protein gene promoter in transgenic wheat seed

Lamacchia, C., Shewry, P. R., Di Fonzo, N., Forsyth, J. L., Harris, N., Lazzeri, P. A., Napier, J. A., Halford, N. G. and Barcelo, P. 2001. Endosperm-specific activity of a storage protein gene promoter in transgenic wheat seed. Journal of Experimental Botany. 52, pp. 243-250.

Genetically modified crops: methodology, benefits, regulation and public concerns

Halford, N. G. and Shewry, P. R. 2000. Genetically modified crops: methodology, benefits, regulation and public concerns. British Medical Bulletin. 56 (1), pp. 62-73.

Antisense expression of a SnRK1 protein kinase sequence in transgenic barley (Hordeum vulgare L.): evidence of an important role for SnRK1 protein kinase in carbohydrate metabolism during pollen development

Zhang, Y., Barcelo, P., Shewry, P. R., Jones, H. D., Davey, M. R., Lazzeri, P. A. and Halford, N. G. 2000. Antisense expression of a SnRK1 protein kinase sequence in transgenic barley (Hordeum vulgare L.): evidence of an important role for SnRK1 protein kinase in carbohydrate metabolism during pollen development. Barley Genetics VIII: Proceedings 8th International Barley Genetics Symposium, Adelaide, October 2000 . pp. 134-136

Improvement of durum wheat quality by genetic engineering

Shewry, P. R., Tosi, P., He, G. Y., Lamacchia, C., Di Fonzo, N., D'ovidio, R., Jones, H. D., Bell, P., Barcelo, P., Napier, J. A., Halford, N. G. and Lazzeri, P. A. 2000. Improvement of durum wheat quality by genetic engineering. Proceedings International Workshop on Durum Wheat, Semolina and Pasta Quality: Recent Achievements and New Trends, Montpellier, 27 November 2000 . pp. 9-15

Expression of gamma-zein, a maize storage protein gene, in barley seed

Zhang, Y., Darlington, H., Napier, J. A., Jones, H. D., Davey, M. R., Halford, N. G., Lazzeri, P. A. and Shewry, P. R. 2000. Expression of gamma-zein, a maize storage protein gene, in barley seed. Barley Genetics VIII,Proceedings 8th International Barley Genetics Symposium, Adelaide, October 2000 Vol. 3. pp. 131-133

Silencing of HMW glutenins in transgenic wheat expressing extra HMW subunits

Alvarez, M. L., Guelman, S., Halford, N. G., Lustig, S., Reggiardo, M. I., Ryabushkina, N., Shewry, P. R., Stein, J. and Vallejos, R. H. 2000. Silencing of HMW glutenins in transgenic wheat expressing extra HMW subunits. Theoretical and Applied Genetics. 100, pp. 319-327.

Method of delaying or inhibiting sprouting in plants. (Patent WO0053746)

Halford, N. G. and Purcell, P. C. 2000. Method of delaying or inhibiting sprouting in plants. (Patent WO0053746).

Hordeum vulgare mRNA for SnRK1-interacting protein 1

Slocombe, S. P., Bertini, L., Beaudoin, F., Dickinson, J. R. and Halford, N. G. 2000. Hordeum vulgare mRNA for SnRK1-interacting protein 1.

Hordeum vulgare snipla gene, partial

Slocombe, S. P., Bertini, L., Beaudoin, F., Dickinson, J. R. and Halford, N. G. 2000. Hordeum vulgare snipla gene, partial.

Reply...The sugar sensing story

Halford, N. G., Purcell, P. C. and Hardie, D. G. 1999. Reply...The sugar sensing story. Trends in Plant Science. 4 (7), p. 251. https://doi.org/10.1016/S1360-1385(99)01432-6

Is hexokinase really a sugar sensor in plants?

Halford, N. G., Purcell, P. C. and Hardie, D. G. 1999. Is hexokinase really a sugar sensor in plants? Trends in Plant Science. 4 (3), pp. 117-120. https://doi.org/10.1016/S1360-1385(99)01377-1

Two SNF1-Related Protein Kinases from Spinach Leaf Phosphorylate and Inactivate 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase, Nitrate Reductase, and Sucrose Phosphate Synthase in Vitro

Sugden, C., Donaghy, P. G., Halford, N. G. and Hardie, D. G. 1999. Two SNF1-Related Protein Kinases from Spinach Leaf Phosphorylate and Inactivate 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase, Nitrate Reductase, and Sucrose Phosphate Synthase in Vitro. Plant Physiology. 120 (1), pp. 257-274. https://doi.org/10.1104/pp.120.1.257

Regulation of spinach SNF1‐related (SnRK1) kinases by protein kinases and phosphatases is associated with phosphorylation of the T loop and is regulated by 5′‐AMP

Sugden, C., Crawford, R. M., Halford, N. G. and Hardie, D. G. 1999. Regulation of spinach SNF1‐related (SnRK1) kinases by protein kinases and phosphatases is associated with phosphorylation of the T loop and is regulated by 5′‐AMP. The Plant Journal. 19 (4), pp. 433-439. https://doi.org/10.1046/j.1365-313X.1999.00532.x

Genetic manipulation of seed composition in barley

Zhang, Y., Darlington, H., Napier, J. A., Jones, H. D., Davey, M. R., Shewry, P. R., Halford, N. G. and Lazzeri, P. A. 1999. Genetic manipulation of seed composition in barley. Abstracts 16th Long Ashton International Symposium on Biotechnology of Cereals: Tools, Targets and Triumphs, Long Ashton, 13-15 September 1999 .

Hordeum vulgare mRNA for SnRK1-type protein kinase, partial

Slocombe, S. P., Bertini, L., Beaudoin, F., Dickinson, J. R. and Halford, N. G. 1998. Hordeum vulgare mRNA for SnRK1-type protein kinase, partial.

Beta vulgaris mRNA for SNF1-related protein kinase

Halford, N. G. 1997. Beta vulgaris mRNA for SNF1-related protein kinase.

S. tuberosum mRNA for SNF1-related protein kinase (1175bp)

Halford, N. G. 1996. S. tuberosum mRNA for SNF1-related protein kinase (1175bp).

S. tuberosum PKIN1 gene (353bp)

Halford, N. G. 1996. S. tuberosum PKIN1 gene (353bp).

S. tuberosum PKIN1 gene (1194bp)

Halford, N. G. 1996. S. tuberosum PKIN1 gene (1194bp).

S. tuberosum PKIN1 gene (802bp)

Halford, N. G. 1996. S. tuberosum PKIN1 gene (802bp).

S. tuberosum PKIN1 gene (668bp)

Halford, N. G. 1996. S. tuberosum PKIN1 gene (668bp).

S. tuberosum PKIN1 gene (258bp)

Halford, N. G. 1996. S. tuberosum PKIN1 gene (258bp).

Serine/threonine-specific protein kinase (EC 2.7.1.-) BKIN2 - barley (fragment)

Hannappel, U., Vicente-Carbajosa, J., Barker, J. H. A., Shewry, P. R. and Halford, N. G. 1996. Serine/threonine-specific protein kinase (EC 2.7.1.-) BKIN2 - barley (fragment).

Analysis of the structures and functional properties of the HMW subunits of glutenin using protein engineering and expression in transgenic plants

Shewry, P. R., Buonocore, F., Bertini, L., Greenfield, J. J. A., Halford, N. G., Tatham, A. S., Rooke, L., Barro, F., Barcelo, P. and Lazzeri, P. A. 1996. Analysis of the structures and functional properties of the HMW subunits of glutenin using protein engineering and expression in transgenic plants. Wrigley, C. W. (ed.) Gluten '96. Proceedings 6th International Wheat Gluten Workshop, Sydney, 2-4 September 1996 . Royal Australian Chemical Institute, North Melbourne. pp. 180-183

Analysis of the structures and functional properties of the HMW subunits of glutenin using protein engineering and expression in transgenic plants

Shewry, P. R., Buonocore, F., Bertini, L., Greenfield, J., Halford, N. G., Tatham, A. S., Rooke, L., Barro, F., Barcelo, P. and Lazzeri, P. A. 1996. Analysis of the structures and functional properties of the HMW subunits of glutenin using protein engineering and expression in transgenic plants. Wrigley, C. W. (ed.) Proceedings 6th International Wheat Gluten Workshop and 46th Australian Cereal Chemistry Conference, Sydney, 2-4 September 1996 . Royal Australian Chemical Institute, North Melbourne. pp. 180-183

Carbon flux in transgenic sugar beet

Monger, W. A., Harrington, P. J., Acaster, M. A., Halford, N. G., Ainsworth, C. C. and Thomas, T. H. 1996. Carbon flux in transgenic sugar beet. in: Fenwick, G. R., Khokhar, S., Richards, R. L. and Hedley, C. (ed.) Agrifood quality - an interdisciplinary approach Royal Society of Chemistry (RSC), Cambridge. pp. 12-14

Regulation of leaf sucrose-phosphate synthase and nitrate reductase by reversible protein phosphorylation

Huber, S. C., Mcmichael, R. W., Bachmann, M., Huber, J. L., Shannon, J. C., Kang, K-K. and Paul, M. J. 1996. Regulation of leaf sucrose-phosphate synthase and nitrate reductase by reversible protein phosphorylation. in: Shewry, P. R., Hooley, R. and Halford, N. G. (ed.) Protein phosphorylation in plants Oxford University Press (OUP) Oxford. pp. 19-34

Structure, assembly and targeting of wheat storage proteins

Shewry, P. R., Sayanova, O., Tatham, A. S., Tamas, L., Turner, M., Richard, G., Hickman, D., Fido, R., Halford, N. G., Greenfield, J., Grimwade, B., Thomson, N., Miles, M., Freedman, R. and Napier, J. 1995. Structure, assembly and targeting of wheat storage proteins. Journal of Plant Physiology. 145 (5-6), pp. 620-625. https://doi.org/10.1016/s0176-1617(11)81273-3

Differential expression of two barley SNF1-related protein-kinase genes

Hannappel, U., Vicentecarbajosa, J., Barker, J. H. A., Shewry, P. R. and Halford, N. G. 1995. Differential expression of two barley SNF1-related protein-kinase genes. Plant Molecular Biology. 27 (6), pp. 1235-1240. https://doi.org/10.1007/bf00020898

The plant-cell cycle

Francis, D. and Halford, N. G. 1995. The plant-cell cycle. Physiologia Plantarum. 93 (2), pp. 365-374. https://doi.org/10.1111/j.1399-3054.1995.tb02241.x

Manipulation of wheat protein quality

Shewry, P. R., Tatham, A. S., Greenfield, J., Halford, N. G., Barro, F., Barcelo, P. and Lazzeri, P. A. 1995. Manipulation of wheat protein quality. Proceedings Royal Society of Chemistry Meeting on Agri-Food Quality '95, Norwich, 25-29 May 1995 .

Opportunities for the improvement of cereals by genetic engineering

Shewry, P. R., Tatham, A. S., Halford, N. G., Barro, F., Barcelo, P. and Lazzeri, P. A. 1995. Opportunities for the improvement of cereals by genetic engineering. Smith, J. M., R, Dixon, K., P, Davies, W., G, Hewitt, H., Hollomon, W. D. and Tyson, D. (ed.) A vital role for fungicides in cereal production . BIOS Scientific Publishers Limited. pp. 29-34

Transformation of cereals: the state of the technology and the expression of gluten proteins genes in transgenic tritordeum and wheat

Lazzeri, P. A., Barro, F., Cannell, M., Salgueiro, S., Shewry, P. R., Tatham, A. S., Halford, N. G. and Barcelo, P. 1995. Transformation of cereals: the state of the technology and the expression of gluten proteins genes in transgenic tritordeum and wheat. Abstracts SEB Symposium on Molecular Physiology of Crop Plants, Versailles, July 1995 .

A transgenic approach towards investigating carbon metabolism in sugar beet

Monger, W., Harrington, P. J., Acaster, M. A., Halford, N. G., Ainsworth, C. C. and Thomas, T. H. 1995. A transgenic approach towards investigating carbon metabolism in sugar beet. Proceedings 58th Congress of the Institut International de Recherches Betteravieres, Beaune, June 1995 . pp. 71-82

Improvement of barley and wheat quality by genetic-engineering

Shewry, P. R., Tatham, A. S., Halford, N. G., Davies, J., Harris, N. and Kreis, M. 1994. Improvement of barley and wheat quality by genetic-engineering. in: Henry, R. J. and Ronalds, J. A. (ed.) Improvement of Cereal Quality by Genetic Engineering Plenum Press Div Plenum Publishing Corp. pp. 79-86

A beta-turn rich barley seed protein is correctly folded in escherichia-coli

Tamas, L., Greenfield, J., Halford, N. G., Tatham, A. S. and Shewry, P. R. 1994. A beta-turn rich barley seed protein is correctly folded in escherichia-coli. Protein Expression And Purification. 5 (4), pp. 357-363. https://doi.org/10.1006/prep.1994.1052

Opportunities for manipulating the seed protein-composition of wheat and barley in order to improve quality

Shewry, P. R., Tatham, A. S., Halford, N. G., Barker, J. H. A., Hannappel, U., Gallois, P., Thomas, M. and Kreis, M. 1994. Opportunities for manipulating the seed protein-composition of wheat and barley in order to improve quality. Transgenic Research. 3 (1), pp. 3-12. https://doi.org/10.1007/bf01976021

Investigating the role of plant SNF1-related protein-kinases

Halford, N. G., Man, A. L., Barker, J. H. A., Monger, W., Shewry, P. R., Smith, A. and Purcell, P. C. 1994. Investigating the role of plant SNF1-related protein-kinases. Biochemical Society Transactions. 22 (4), pp. 953-957. https://doi.org/10.1042/bst0220953

SNF1-related protein-kinase genes in cereals

Halford, N. G. 1993. SNF1-related protein-kinase genes in cereals. in: Shewry, P. R. and Stobart, K. (ed.) Seed Storage Compounds: Biosynthesis, Interactions and Manipulations Oxford University Press.

Tobacco plants transformed with cdc25, a mitotic inducer gene from fission yeast

Bell, M. H., Halford, N. G., Ormrod, J. C. and Francis, D. 1993. Tobacco plants transformed with cdc25, a mitotic inducer gene from fission yeast. Plant Molecular Biology. 23 (3), pp. 445-451. https://doi.org/10.1007/bf00019293

Development of expression and refolding systems for cereal seed storage proteins.

Shewry, P. R., Laszlo, T., Tatham, A. S., Halford, N. G., Pratt, K., Madgwick, P. J., Thompson, S. and Bishop, D. H. L. 1993. Development of expression and refolding systems for cereal seed storage proteins. Goodenough, P. (ed.) Protein Engineering II.Proceedings of the 2nd AFRC Protein Engineering Conference, Cambridge, September 1992 . CPL Scientific, Newbury. pp. 40-51

Molecular cloning of a novel barley seed protein gene that is repressed by abscisic acid

Liu, R., Olsen, O. A., Kreis, M. and Halford, N. G. 1992. Molecular cloning of a novel barley seed protein gene that is repressed by abscisic acid. Plant Molecular Biology. 18 (6), pp. 1195-1198. https://doi.org/10.1007/bf00047726

Molecular analyses of a barley multigene family homologous to the yeast protein kinase gene SNF1

Halford, N. G., Vicentecarbajosa, J., Sabelli, P. A., Shewry, P. R., Hannappel, U. and Kreis, M. 1992. Molecular analyses of a barley multigene family homologous to the yeast protein kinase gene SNF1. The Plant Journal. 2 (5), pp. 791-797. https://doi.org/10.1111/j.1365-313X.1992.tb00148.x

Identification of a novel beta-turn-rich repeat motif in the D hordeins of barley

Halford, N. G., Tatham, A. S., Sui, E., Daroda, L., Dreyer, T. and Shewry, P. R. 1992. Identification of a novel beta-turn-rich repeat motif in the D hordeins of barley. Biochimica et Biophysica Acta. 1122 (2), pp. 118-122. https://doi.org/10.1016/0167-4838(92)90313-3

High-molecular-weight subunits of wheat glutenin

Shewry, P. R., Halford, N. G. and Tatham, A. S. 1992. High-molecular-weight subunits of wheat glutenin. Journal of Cereal Science. 15 (2), pp. 105-120. https://doi.org/10.1016/s0733-5210(09)80062-3

Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality

Halford, N. G., Field, J. M., Blair, H., Urwin, P., Moore, K., Robert, L., Thompson, R., Flavell, R. B., Tatham, A. S. and Shewry, P. R. 1992. Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theoretical and Applied Genetics. 83 (3), pp. 373-378. https://doi.org/10.1007/BF00224285

Isolation and characterization of genes encoding rye prolamins containing a highly repetitive sequence motif

Hull, G. A., Halford, N. G., Kreis, M. and Shewry, P. R. 1991. Isolation and characterization of genes encoding rye prolamins containing a highly repetitive sequence motif. Plant Molecular Biology. 17 (5), pp. 1111-1115. https://doi.org/10.1007/bf00037153

Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein-kinase cDNA

Alderson, A., Sabelli, P. A., Dickinson, J. R., Cole, D., Richardson, M., Kreis, M., Shewry, P. R. and Halford, N. G. 1991. Complementation of snf1, a mutation affecting global regulation of carbon metabolism in yeast, by a plant protein-kinase cDNA. Proceedings of the National Academy of Sciences. 88 (19), pp. 8602-8605. https://doi.org/10.1073/pnas.88.19.8602

The isolation and characterisation of protein kinase-encoding cDNA and genomic clones from rye and barley

Halford, N. G., Shewry, P. R., Sabelli, P., Alderson, A., Richardson, M. and Kreis, M. 1990. The isolation and characterisation of protein kinase-encoding cDNA and genomic clones from rye and barley. Annual Meeting of the Society of Experimental Biology, Warwick, 1990. Abstract. pp. C1-6

Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne

Anderson, O. D., Greene, F. C., Yip, R. E., Halford, N. G., Shewry, P. R. and Malpica-Romero, J. M. 1989. Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne. Nucleic Acids Research. 17 (1), pp. 461-462. https://doi.org/10.1093/nar/17.1.461

The structure and functionality of wheat gluten proteins.

Shewry, P. R., Halford, N. G., Field, J. M. and Tatham, A. S. 1989. The structure and functionality of wheat gluten proteins. Proceedings of the 38th Australian Cereal Chemistry Division, Sydney. . pp. 113-116

Functional analysis of the upstream regions of a silent and an expressed member of a family of wheat seed protein genes in transgenic tobacco.

Halford, N. G., Forde, J., Shewry, P. R. and Kreis, M. 1989. Functional analysis of the upstream regions of a silent and an expressed member of a family of wheat seed protein genes in transgenic tobacco. Plant Science. *^62\*, pp. 207-216.

The structure and functionality of wheat gluten proteins.

Shewry, P. R., Halford, N. G., Field, J. M. and Tatham, A. S. 1989. The structure and functionality of wheat gluten proteins.  Proceedings of the 38th Australian Cereal Chemistry Conference, Sydney, 1988. Royal Australian Chemical Institute, Cereal Chemistry Division, Sydney..

Structural and molecular analyses of wheat proteins that determine breadmaking quality.

Shewry, P. R., Tatham, A. S., Halford, N. G., Field, J. M., Belton, P. S., Anderson, O. and Greene, F. C. 1988. Structural and molecular analyses of wheat proteins that determine breadmaking quality.

Analysis of possible quality-related sequence variations in the 1D glutenin high molecular weight subunit genes of wheat.

Greene, F. C., Anderson, O. D., Yip, R. E., Halford, N. G., Malpica Romero, J. M. and Shewry, P. R. 1988. Analysis of possible quality-related sequence variations in the 1D glutenin high molecular weight subunit genes of wheat. Proceedings of the 7th International Wheat Genetics Symposium, Cambridge, 1988. Vol. 1. T.E. Miller & R.M.D. Koebner eds. pp. 735-740

Structure and analysis of the high molecular weight glutenin genes from Triticum aestivum L. cv Cheyenne.

Anderson, O. D., Halford, N. G., Forde, J., Yip, R. E., Shewry, P. R. and Greene, F. C. 1988. Structure and analysis of the high molecular weight glutenin genes from Triticum aestivum L. cv Cheyenne. Proceedings of the 7th International Wheat Genetics Symposium, Cambridge, 1988. Vol. 1. T.E. Miller & R.M.D. Koebner eds. pp. 699-704

The structure and expression of genes encoding the high molecular weight (HMW) subunits of wheat glutenin.

Halford, N. G., Forde, J., Anderson, O. D., Greene, F. C. and Shewry, P. R. 1988. The structure and expression of genes encoding the high molecular weight (HMW) subunits of wheat glutenin. Proceedings of the 7th International Wheat Genetics Symposium, Cambridge, 1988. Vol. 1. T.E. Miller & R.M.D. Koebner eds. pp. 745-750

Molecular cloning of the barley seed protein CMd: a variant member of the alpha-amylase/trypsin inhibitor family of cereals.

Halford, N. G., Morris, N. A., Urwin, P., Williamson, M. S., Kasarda, D. D., Lew, E. J-L., Kreis, M. and Shewry, P. R. 1988. Molecular cloning of the barley seed protein CMd: a variant member of the alpha-amylase/trypsin inhibitor family of cereals. Biochimica et Biophysica Acta (BBA) - Gene structure and expression. 950 (3), pp. 435-440. https://doi.org/10.1016/0167-4781(88)90141-8

The molecular genetics of the high molecular weight subunits of wheat glutenin.

Halford, N. G., Forde, J. and Shewry, P. R. 1988. The molecular genetics of the high molecular weight subunits of wheat glutenin. Proceedings 3rd International Workshop on Gluten Proteins, Budapest, 1987 . Eds R. Lasztity & F. Bekes. Singapore : World Scientific Publishing Co.. pp. 3-12

The structures of barley and wheat prolamins and their genes.

Shewry, P. R., Tatham, A. S., Field, J. M., Forde, B. G., Clark, J., Gallois, P., Marris, C., Halford, N. G., Forde, J. and Kreis, M. 1988. The structures of barley and wheat prolamins and their genes. Biochemie und Physiologie der Pflanzen. 183, pp. 117-127.

The structures of barley and wheat prolamins and their genes.

Shewry, P. R., Tatham, A. S., Field, J. M., Forde, B. G., Clark, J., Gallois, P., Marris, C., Halford, N. G., Forde, J. and Kreis, M. 1988. The structures of barley and wheat prolamins and their genes.

The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L. ), and comparison with those of genes from chromosome 1A and 1D.

Halford, N. G., Forde, J., Anderson, O. D., Greene, F. C. and Shewry, P. R. 1987. The nucleotide and deduced amino acid sequences of an HMW glutenin subunit gene from chromosome 1B of bread wheat (Triticum aestivum L. ), and comparison with those of genes from chromosome 1A and 1D. Theoretical and Applied Genetics. 75, pp. 117-126.

The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.)

Forde, J., Malpica, J-M., Halford, N. G., Shewry, P. R., Anderson, O. D., Greene, F. C. and Miflin, B. J. 1985. The nucleotide sequence of a HMW glutenin subunit gene located on chromosome 1A of wheat (Triticum aestivum L.). Nucleic Acids Research. 13 (19), pp. 6817-7339. https://doi.org/10.1093/nar/13.19.6817

Projects

21ENGBIO: Re-engineering amino acid metabolism in wheat grain

BBSRC LINK: Genetic improvement of wheat to reduce the potential for acrylamide formation during processing

BBSRC LINK: Producing low acrylamide risk potatoes

Collaborative Training Partnership Studentship with Mondelez International

Crop management strategies for low asparagine grains to limit acrylamide-forming potential

Defining the signalling network linking pathogen infection and asparagine accumulation in wheat grain

Field assessment of ultra-low asparagine, low acrylamide, gene edited wheat

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